Motility of sturgeon spermatozoa can sustain successive activations episodes

Dzyuba, Borys; Cosson, Jacky; Boryshpolets, Sergii; Dzyuba, Viktoriya; Rodina, Marek; Bondarenko, Olga; Shaliutina, Anna; Linhart, Otomar

doi:10.1016/j.anireprosci.2013.02.011

Cited by 12 publications

(4 citation statements)

References 28 publications

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“…On the other hand, in species with external fertilization, like fish, alternatives to subpopulation structures have been proposed (Fitzpatrick et al 2007;Dzyuba et al 2013;Linhartova et al 2013). This opens a very interesting scientific and intellectual approach in the evolutive perspective, but it is out of the interest of the present work more centred on farm conditions.…”

Section: Discussionmentioning

confidence: 99%

Kinematics and Subpopulations’ Structure Definition of Blue Fox (Alopex lagopus) Sperm Motility using the ISAS^®V1 CASA System

Soler

Garcia

Contell

et al. 2014

Reprod Domestic Animals

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Over recent years, technological advances have brought innovation in assisted reproduction to the agriculture. Fox species are of great economical interest in some countries, but their semen characteristics have not been studied enough. To advance the knowledge of function of fox spermatozoa, five samples were obtained by masturbation, in the breeding season. Kinetic analysis was performed using ISAS® v1 system. Usual kinematic parameters (VCL, VSL, VAP, LIN, STR, WOB, ALH and BCF) were considered. To establish the standardization for the analysis of samples, the minimum number of cells to analyse and the minimum number of fields to capture were defined. In the second step, the presence of subpopulations in blue fox semen was analysed. The minimum number of cells to test was 30, because kinematic parameters remained constant along the groups of analysis. Also, the effectiveness of ISAS® D4C20 counting chamber was studied, showing that the first five squares presented equivalent results, while in the squares six and seven, the kinematic parameters showed a reduction in all of them, but not in the concentration or motility percentage. Kinematic variables were grouped into two principal components (PC). A linear movement characterized PC1, while PC2 showed an oscillatory movement. Three subpopulations were found, varying in structure among different animals.

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Section: Discussionmentioning

confidence: 99%

Kinematics and Subpopulations’ Structure Definition of Blue Fox (Alopex lagopus) Sperm Motility using the ISAS^®V1 CASA System

Soler

Garcia

Contell

et al. 2014

Reprod Domestic Animals

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show abstract

“…The value for spermatozoon VCL at 10 s post activation without reagents was in accordance with Dadras et al . (2019), but low compared to other published data on sturgeon (Dzyuba et al ., 2013,b; Xin et al ., 2018). This difference may be attributed to factors previously reported to influence the motility of fish spermatozoa: composition of AM, dilution rate, ions, pH and osmolality (Alavi et al ., 2004a, 2004b; Alavi & Cosson, 2006).…”

Section: Discussionmentioning

confidence: 99%

Energy pathways associated with sustained spermatozoon motility in the endangered Siberian sturgeon Acipenser baerii

Rahi¹,

Dzyuba²,

Xin³

et al. 2020

Journal of Fish Biology

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Sturgeon spermatozoa are unique for their sustained motility. We investigated the relative importance of bioenergetic pathways in the energy supply of Siberian sturgeon Acipenser baerii spermatozoa during motile and immotile states. Spermatozoon motility and oxygen consumption rate (OCR) were analysed following exposure to inhibitors of oxidative phosphorylation (sodium azide, NaN 3), glycolysis (2-deoxy-Dglucose, DOG) and β-oxidation of fatty acids (sodium fluoride, NaF), and to an uncoupler of oxidative phosphorylation (carbonyl cyanide m-chlorophenyl hydrazine, CCCP). No significant difference in curvilinear velocity was observed after addition of these reagents to activation medium (AM) or nonactivation medium (NAM) for incubation. Incubation of spermatozoa in NAM containing CCCP or NaN 3 resulted in significantly decreased motility duration compared to controls. The OCR of sturgeon spermatozoa in AM (11.9 ± 1.4 nmol O 2 min −1 (10 9 spz) −1) was significantly higher than in NAM (8.2 ± 1.5 nmol O 2 min −1 (10 9 spz) −1). The OCR significantly declined with addition of NaN 3 to AM and NAM. No significant difference in motility parameters or OCR was observed with NaF or DOG. These results suggest active oxidative phosphorylation in both immotile and motile spermatozoa. Nevertheless, mitochondrial respiration occurring during motility is not sufficient to meet the high energy demands, and the energy required for sustained motility of Siberian sturgeon spermatozoa is derived from adenosine triphosphate accumulated during the quiescent state.

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“…Seminal plasma osmolarity has previously been shown to affect sperm motility and activity in invertebrates and vertebrates [12]. The motility of spermatozoa is primarily influenced by changes in the surrounding ionic contents, osmolality, and pH [13,14]. In sturgeon fish, a negative correlation was found between the osmolality of semen and the percentage of sperm motility [15].…”

Section: Introductionmentioning

confidence: 99%

Relationships of Osmolality and Oxidative Stress with Semen Quality and Their Effects on Male Fertility

Ameen¹

2021

IHJPAS

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The objective of this study was to investigate and find the relationships between human semen osmolality and oxidative stress with semen quality. Semen analysis was determined to evaluate semen quality and fertility. The concentration of semen malondialdehyde (MDA) was measured to assess oxidative stress. For this purpose, one hundred seventy healthy adult males were used in this study. The study was conducted from September 2018 to November 2019 in the Infertility care and in vitro fertilization center (IVF) and the Biology department of the College of Science at Salahaddin University in Erbil city. A significant positive correlation was observed between the total sperm motility, grade activity, and sperm motility index with semen osmolality less than 300 mOsm kg-1 (r= 0.62, r = 0.64, and r= 0.75 pâ‰¤0.01 respectively) and osmolality 300-350 (r= 0.53, pâ‰¤0.05 r= 0.52 pâ‰¤0.05, and r= 0.56 pâ‰¤0.01 respectively). Total sperm motility, grade activity, and sperm motility index are negatively correlated with osmolality 351-400 mOsm kg-1 (r= -0.65 pâ‰¤0.05, r= -0.56 pâ‰¤0.05, and r= -0.67 pâ‰¤0.01) and more than 400 mOsm kg-1 (r= -0.86, r = -0.74 and r= -0.88 pâ‰¤0.01). Regarding the relation between oxidative stress and sperm motility kinetics, total sperm motility, grade activity, and sperm motility index is negatively correlated with MDA more than 2 Âµmol/L (r= -0.56 pâ‰¤0.05, r= -0.52 pâ‰¤0.05, and r= -0.67 pâ‰¤0.01 respectively). No significant correlation was found between semen osmolality and MDA concentration with sperm concentration, total sperm count, sperm viability, and normal sperm morphology.

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Motility of sturgeon spermatozoa can sustain successive activations episodes

Cited by 12 publications

References 28 publications

Kinematics and Subpopulations’ Structure Definition of Blue Fox (Alopex lagopus) Sperm Motility using the ISAS^®V1 CASA System

Kinematics and Subpopulations’ Structure Definition of Blue Fox (Alopex lagopus) Sperm Motility using the ISAS^®V1 CASA System

Energy pathways associated with sustained spermatozoon motility in the endangered Siberian sturgeon Acipenser baerii

Relationships of Osmolality and Oxidative Stress with Semen Quality and Their Effects on Male Fertility

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Motility of sturgeon spermatozoa can sustain successive activations episodes

Cited by 12 publications

References 28 publications

Kinematics and Subpopulations’ Structure Definition of Blue Fox (Alopex lagopus) Sperm Motility using the ISAS®V1 CASA System

Kinematics and Subpopulations’ Structure Definition of Blue Fox (Alopex lagopus) Sperm Motility using the ISAS®V1 CASA System

Energy pathways associated with sustained spermatozoon motility in the endangered Siberian sturgeon Acipenser baerii

Relationships of Osmolality and Oxidative Stress with Semen Quality and Their Effects on Male Fertility

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Product

Resources

About

Kinematics and Subpopulations’ Structure Definition of Blue Fox (Alopex lagopus) Sperm Motility using the ISAS^®V1 CASA System

Kinematics and Subpopulations’ Structure Definition of Blue Fox (Alopex lagopus) Sperm Motility using the ISAS^®V1 CASA System