Motility Subpopulations with Distinct Motility Characteristics Using Swim-Up-Selected Sperm Cells from Norwegian Red Bulls: Effects of Freezing–Thawing and Between-Bull Variation

Alm-Kristiansen, Anne Hege

doi:10.3390/biology12081086

Cited by 2 publications

(1 citation statement)

References 42 publications

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“…Despite literature findings, our work suggests a possible positive effect of the used concentration – far below the toxic limit – of CYP on sperm motility. Indeed, such a used concentration enhances the effect of incubation time and temperature alone (CTRL), generally associated with an increase in sperm velocities ( 46 , 65 ). According to the velocity outcome, the time-window response of CYP is from

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, suggesting that the action of the drug occurs before the end of the entire observation time, since an evident reduction of cell velocity occurs at

60

.…”

Section: Discussionmentioning

confidence: 99%

Exploring altered bovine sperm trajectories by sperm tracking in unconfined conditions

Canonico,

De Clemente,

Fardilha

et al. 2024

Front. Vet. Sci.

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Mammalian sperm motility is getting more relevant due to rising infertility rates worldwide, generating the need to improve conventional analysis and diagnostic approaches. Nowadays, computer assisted sperm analysis (CASA) technologies represent a popular alternative to manual examination which is generally performed by observing sperm motility in very confined geometries. However, under physiological conditions, sperm describe three-dimensional motility patterns which are not well reconstructed by the limited depth of standard acquisition chambers. Therefore, affordable and more versatile alternatives are needed. Here, a motility analysis in unconfined conditions is proposed. In details, the analysis is characterized by a significant longer duration -with respect to conventional systems- with the aim to observe eventually altered motility patterns. Brightfield acquisition in rectangular glass capillaries captured frozen–thawed bovine spermatozoa which were analyzed by means of a self-written tracking routine and classified in sub-populations, based on their curvilinear velocity. To test the versatility of our approach, cypermethrin -a commonly used pesticides- known to be responsible for changes in sperm motility was employed, assessing its effect at three different time-steps. Experimental results showed that such drug induces an increase in sperm velocity and progressiveness as well as circular pattern formation, likely independent of wall interactions. Moreover, this resulted in a redistribution of sperm with the rapid class declining in number with time, but still showing an overall velocity increase. The flexibility of the approach permits parameter modifications with the experimental needs, allowing us to conduct a comprehensive examination of sperm motility. This adaptability facilitated data acquisition which can be computed at different frame rates, extended time periods, and within deeper observation chambers. The suggested approach for sperm analysis exhibits potential as a valuable augmentation to current diagnostic instruments.

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10

30

, suggesting that the action of the drug occurs before the end of the entire observation time, since an evident reduction of cell velocity occurs at

60

.…”

Section: Discussionmentioning

confidence: 99%

Exploring altered bovine sperm trajectories by sperm tracking in unconfined conditions

Canonico,

De Clemente,

Fardilha

et al. 2024

Front. Vet. Sci.

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Predictive Indicators of Cryotolerance and Fertility in Bovine Sperm: Evaluating Fresh Semen Quality to Improve AI Outcomes With Frozen–Thawed Sperm

Tamargo,

Garriga,

Yeste

et al. 2024

Reprod Domestic Animals

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The success of artificial insemination (AI) with frozen–thawed semen in cattle is influenced by both female factors and sperm quality. In terms of sperm quality, prior studies indicate that the ability of frozen–thawed bovine sperm to fertilise an oocyte is dependent on their quality and resilience to cryopreservation. Cryopreservation induces oxidative stress, leading to ultrastructural damage in the sperm. This study aimed to determine whether the quality of fresh semen can identify bulls with good and poor sperm freezability. This difference between fresh and frozen semen from the same bull allows us to predict fertility. Motility and kinetic parameters were assessed using computer‐assisted sperm analysis (CASA), while six functional variables were evaluated through flow cytometry, both before and after the freeze–thaw process on the sperm from 13 bulls. In vivo fertility was measured using 90‐day non‐return rates. The principal component analysis (PCA) of eight sperm variables post‐thaw identified one principal component explaining 81.19% of the total variance and classified the bulls into two groups: Poor freezability bulls (progressive motility: 48.12% ± 8.41%; viability: 77.51% ± 7.61%) and good freezability bulls (progressive motility: 58.64% ± 6.64%; viability: 88.12% ± 2.52%). Bulls with higher freezability showed better sperm viability and motility, as well as lower levels of ROS, superoxides and intracellular calcium before cryopreservation that were significantly correlated with higher non‐return rates (NRR). The results underscore the importance of assessing the quality and functionality of fresh semen to predict the fertility potential of cryopreserved sperm. This approach can aid in selecting ejaculates with the best potential for successful artificial insemination, ultimately improving reproductive performance in dairy cattle.

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Motility Subpopulations with Distinct Motility Characteristics Using Swim-Up-Selected Sperm Cells from Norwegian Red Bulls: Effects of Freezing–Thawing and Between-Bull Variation

Cited by 2 publications

References 42 publications

Exploring altered bovine sperm trajectories by sperm tracking in unconfined conditions

Exploring altered bovine sperm trajectories by sperm tracking in unconfined conditions

Predictive Indicators of Cryotolerance and Fertility in Bovine Sperm: Evaluating Fresh Semen Quality to Improve AI Outcomes With Frozen–Thawed Sperm

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