“…technologies have brought significant contributions to the field of microfluidics for precision manipulation techniques, [1][2][3][4] notably due to their relatively low power requirement and superior biocompatibility. 5,6 Ultrasonic actuators have demonstrated the ability to accomplish various microfluidic functions such as separation, 3,[7][8][9][10][11] patterning, [12][13][14][15][16][17] trapping, [18][19][20][21] focusing, 22,23 concentrating, [24][25][26] and guiding [27][28][29] of particles and analytes, which are crucial functions for biomedical applications. To achieve such results, it is often required to enhance and confine the acoustic field near the analytes, which, at this scale, is a challenging endeavor.…”