The purpose of this study is to understand the differences in the academic motivation of students majoring in physical education (PE), fine arts, music, and other disciplines, and the effect of academic motivation on major satisfaction and regret. A total of 898 valid questionnaires were collected from students at five universities in Taiwan, including 203 PE, 207 fine arts, 201 music, and 287 other majors (including Social science and Science, Technology, and Engineering). All participants completed questionnaires measuring motivation, academic major satisfaction, and decision regret. The results revealed that the intrinsic motivation of students in PE, fine arts, and music majors was significantly higher than those in other majors. Furthermore, major satisfaction was positively correlated with intrinsic motivation and identified regulation and negatively correlated with external regulation and amotivation. The opposite was true for the relationship between regret and the various motivation types. The research findings can serve as a reference for career counseling in related departments.