Objective: to analyze the organ donation culture in a hospital population. Methods: descriptive cross-sectional study conducted at the Zone General Hospital with Family Medicine No. 1 of the Mexican Institute of Social Security in Pachuca, Hidalgo, Mexico; from December 2023 to May 2024. A non-probabilistic sample composed by 384 people. The validated questionnaire “International Donor Collaborative Project on Organ Donation and Transplantation” was applied. Univariate analysis was performed using the spss v. 23 package to obtain simple frequencies and percentages. Results: it was identified that 75.3% of the participants would be willing to donate their organs, while 89.6%, and 84.9% would donate a kidney and part of the liver to a relative, respectively. In addition, 40.1% chose reciprocity as the reason why they would donate their organs, while 61.2% considered the possibility of donating the organs of a family member. Regarding the perceptions of their relatives on the subject, 66.7% said they did not know their father’s opinion, while 62.5%, and 66.4% did not know their mother’s and partner’s opinion, respectively. Conclusion: the culture of organ donation was favorable in three quarters of the respondents; this percentage increases when it comes to donating for a family member. The main reason why respondents would be willing to donate their organs was reciprocity.