Mouse AMACO, a kidney and skin basement membrane associated molecule that mediates RGD-dependent cell attachment

Gebauer, Jan; Keene, Douglas R.; Olsen, Bjørn R.; Sorokin, Lydia; Paulsson, Mats; Wagener, Raimund

doi:10.1016/j.matbio.2009.07.006

Cited by 14 publications

(18 citation statements)

References 23 publications

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“…Previous ultrastructural examinations demonstrated that Fras1, Frem1, Frem2 and AMACO are all localized at the BM zone (Gebauer et al , 2009; Dalezios et al , 2007; Petrou et al , 2007a; Petrou et al , 2007b). To determine co-localization of Fras1 and AMACO, double immunoelectron microscopy labeling was performed on P0 mouse skin using secondary antibodies coupled to different sized gold particles.…”

Section: Resultsmentioning

confidence: 97%

“…Immunofluorescence analysis was performed using standard protocols. Primary antibodies used were: rabbit anti-zebrafish AMACO (Gebauer et al, 2010), rabbit anti-zebrafish Fras1 (Carney et al , 2010), rabbit anti-mouse AMACO (Gebauer et al , 2009), and rabbit anti-laminin (Sigma Aldrich, L9393). Images were captured on a Zeiss Axiophot, Zeiss Apotome, Zeiss Confocal (LSM710 META) or Leica M165 FC compound microscope.…”

Section: Methodsmentioning

confidence: 99%

“…All proteins were from mouse. Expression and purification of AMACO fragments has been described earlier (Gebauer et al, 2009) and purity of the proteins is documented in (Supplementary Figure S3). Fras1 CSPG (2200 RUs), AMACO full-length (2300 RUs), AMACO P1 (2500 RUs), AMACO P2 (2400 RUs), and AMACO P3 (2500 RUs) were covalently coupled to carboxymethyldextran hydrogel 500M sensor chips (XanTec, Düsseldorf, Germany) using the amine coupling kit (GE Healthcare).…”

Section: Methodsmentioning

confidence: 99%

“…Tissues were immersed in a combination of affinity purified rabbit AMACO-P3 antibody (Gebauer et al, 2009) or collagen VII antibody (Lunstrum et al, 1986) with guinea pig Fras1 CSPG antibody (Supplementary Figure S2) in Dulbecco’s Modified Eagle Media (DMEM) at a ratio of 1:1:4 overnight at 4°C. Tissues were washed in DMEM for 4 hours at 4°C and then immersed in a combination of goat anti-rabbit 10-nm and goat anti-guinea pig 6-nm colloidal gold conjugates in DMEM at a ratio of 1:1:3 overnight at 4°C.…”

Section: Methodsmentioning

confidence: 99%

“…At the C-terminus another EGF-like domain and a unique domain are present (Sengle et al , 2003; Gebauer et al ., 2008). Analysis of mice and zebrafish revealed specific AMACO localization at the BM of multiple tissues during development (Gebauer et al , 2010; Gebauer et al , 2009; Sengle et al , 2003). We demonstrate that AMACO is lost from both zebrafish and mouse mutants that lack Fras1 and that Fras1 and AMACO interact directly.…”

Section: Introductionmentioning

confidence: 99%

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AMACO Is a Component of the Basement Membrane–Associated Fraser Complex

Richardson

Gebauer

Zhang

et al. 2014

Journal of Investigative Dermatology

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Fraser syndrome (FS) is a phenotypically variable, autosomal recessive disorder characterized by cryptophthalmus, cutaneous syndactyly and other malformations resulting from mutations in FRAS1, FREM2 and GRIP1. Transient embryonic epidermal blistering causes the characteristic defects of the disorder. Fras1, Frem1 and Frem2 form the extracellular Fraser complex, which is believed to stabilize the basement membrane (BM). However, several cases of FS could not be attributed to mutations in FRAS1, FREM2 or GRIP1, while Fraser syndrome displays high clinical variability, suggesting there is an additional genetic, possibly modifying contribution to this disorder. AMACO, encoded by the VWA2 gene, has a very similar tissue distribution to the Fraser complex proteins in both mouse and zebrafish. Here, we show that AMACO deposition is lost in Fras1 deficient zebrafish and mice and that Fras1 and AMACO interact directly via their CSPG and P2 domains. Knockdown of vwa2, which alone causes no phenotype, enhances the phenotype of hypomorphic Fras1 mutant zebrafish. Together, our data suggest that AMACO represents a novel member of the Fraser complex.

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Section: Resultsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

AMACO Is a Component of the Basement Membrane–Associated Fraser Complex

Richardson

Gebauer

Zhang

et al. 2014

Journal of Investigative Dermatology

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Structural and biophysical characterization of the type VII collagen vWFA2 subdomain leads to identification of two binding sites

et al. 2020

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Type VII collagen is an extracellular matrix protein, which is important for skin stability; however, detailed information at the molecular level is scarce. The second vWFA (von Willebrand factor type A) domain of type VII collagen mediates important interactions, and immunization of mice induces skin blistering in certain strains. To understand vWFA2 function and the pathophysiological mechanisms leading to skin blistering, we structurally characterized this domain by X‐ray crystallography and NMR spectroscopy. Cell adhesion assays identified two new interactions: one with β1 integrin via its RGD motif and one with laminin‐332. The latter interaction was confirmed by surface plasmon resonance with a KD of about 1 mm. These data show that vWFA2 has additional functions in the extracellular matrix besides interacting with type I collagen.

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Transcriptome analysis of the regenerating tail vs. the scarring limb in lizard reveals pathways leading to successful vs. unsuccessful organ regeneration in amniotes

Vitulo

Valle

Skobo

et al. 2017

Developmental Dynamics

179

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The AEP and the spinal cord in the tail maintains Wnt and fibroblast growth signaling that stimulate blastema cell proliferation and growth while these signals are absent in the limb as a consequence of the intense inflammation. Regeneration of amniote appendages requires a control of cell proliferation and inflammatory-immune reactions to form an apical epidermal cap. Genes that control cell proliferation and inflammation, addressing regeneration and not tumor formation in the tail and scarring in the limb are discussed for future studies. Developmental Dynamics 246:116-134, 2017. © 2016 Wiley Periodicals, Inc.

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Mouse AMACO, a kidney and skin basement membrane associated molecule that mediates RGD-dependent cell attachment

Cited by 14 publications

References 23 publications

AMACO Is a Component of the Basement Membrane–Associated Fraser Complex

AMACO Is a Component of the Basement Membrane–Associated Fraser Complex

Structural and biophysical characterization of the type VII collagen vWFA2 subdomain leads to identification of two binding sites

Transcriptome analysis of the regenerating tail vs. the scarring limb in lizard reveals pathways leading to successful vs. unsuccessful organ regeneration in amniotes

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