Mouse blastocyst outgrowth and implantation rates following exposure to ethanol or A23187 during culture in vitro

Stachecki, James J.; Yelian, Frank D.; Leach, Richard E.; Armant, D. Randall

doi:10.1530/jrf.0.1010611

Cited by 54 publications

(40 citation statements)

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“…Although this temporal shift occurred, there were no visual, physical abnormalities observed in the developing embryos. Studies in mice have shown that increased levels of calcium can accelerate early blastocyst morphogenesis processes (Stachecki et al, 1994), demonstrating that temporal shifts in development are possible as a consequence of changes in calcium levels. Our data show specifically that decreased environmental calcium concentration might be affecting processes in early development (i.e.…”

Section: Influence Of Environmental Calcium On Embryogenesis and Embrmentioning

confidence: 99%

Consequences of calcium decline on the embryogenesis and life history of Daphnia magna

Giardini

Yan

Heyland

2015

Journal of Experimental Biology

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Ambient calcium is declining in thousands of soft-water lake habitats in temperate regions as a consequence of unsustainable forestry practices, decreased atmospheric calcium deposition and acidic deposition. As their exoskeleton is heavily reinforced with calcium, freshwater crustaceans have a high specific calcium requirement relative to other aquatic organisms. Daphnia, in particular, is an ideal crustacean for investigating the consequences of calcium decline because it is an abundant and important member of freshwater zooplankton communities. Although it has been established that adult and juvenile Daphnia have different tolerances to low ambient calcium as a result of their different life stage-specific calcium requirements, the consequences of declining calcium on embryonic development have never been investigated. Here, we describe the distribution of calcium in embryonic stages of D. magna and introduce a novel and easy to use staging scheme. We tested whether calcium can be traced from mothers to their offspring. Finally, we assessed the fitness consequences of maternal provisioning in limiting calcium environments. We found that while embryos require calcium for their development and moulting, they do not equilibrate with environmental calcium levels. Instead, we were able to trace calcium from mothers to their offspring. Furthermore, our data strongly suggest that females are faced with an allocation trade-off between providing calcium to their offspring and using it for growth and moulting. Together, these data provide novel insights into the consequences of calcium decline for freshwater zooplankton.

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Section: Influence Of Environmental Calcium On Embryogenesis and Embrmentioning

confidence: 99%

Consequences of calcium decline on the embryogenesis and life history of Daphnia magna

Giardini

Yan

Heyland

2015

Journal of Experimental Biology

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“…An endogenous increase in Ca 2ϩ -releasing activity leading to Ca 2ϩ transients was observed during the first mitotic division (21). Moreover, the rate of cavitation and cell division was either accelerated or delayed by experimentally induced elevation or reduction of [Ca 2ϩ ] i , respectively (22)(23)(24). Stacheki and Armant (24) suggested that in mouse embryos, inositol 1,4,5-trisphosphate-sensitive and ryanodinesensitive Ca 2ϩ stores exist at the morula stage and that calmodulin is involved in mediating the Ca 2ϩ signaling effects to its distal targets.…”

mentioning

confidence: 99%

Increase of intracellular Ca ²⁺ and relocation of E-cadherin during experimental decompaction of mouse embryos

Pey

Vial

Schatten

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

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To determine the role of intracellular Ca 2؉ in compaction, the first morphogenetic event in embryogenesis, we analyzed preimplantation mouse embryos under several decompacting conditions, including depletion of extracellular Ca 2؉ , blocking of Ca 2؉ channels, and inhibition of microfilaments, calmodulin, and intracellular Ca 2؉ release. Those treatments induced decompaction of mouse morulae and simultaneously induced changes in cytosolic free Ca 2؉ concentration and deregionalization of E-cadherin and fodrin. When morulae were allowed to recompact, the location of both proteins recovered. In contrast, actin did not change its cortical location with compaction nor with decompaction-recompaction. Calmodulin localized in areas opposite to cell-cell contacts in eight-cell stage embryos before and after compaction. Inhibition of calmodulin with trifluoperazine induced its delocalization while morulae decompacted. A nonspecific rise of intracellular free Ca 2؉ provoked by ionomycin did not affect the compacted shape. Moreover, the same decompacting treatments when applied to uncompacted embryos did not produce any change in intracellular Ca 2؉ . Our results demonstrate that in preimplantation mouse embryos experimentally induced stage-specific changes of cell shape are accompanied by changes of intracellular free Ca 2؉ and redistribution of the cytoskeleton-related proteins E-cadherin, fodrin, and calmodulin. We conclude that intracellular Ca 2؉ specifically is involved in compaction and probably regulates the function and localization of cytoskeleton elements.Extracellular calcium is crucial during the preimplantation development of mammalian embryos because of its role in cell-cell adhesion (1-5). Intracellular free calcium ([Ca 2ϩ ] i ) plays a pivotal role in triggering early events related to fertilization, namely, oocyte maturation, cortical granule exocytosis, and egg activation (6-19). However, little is known about the role of [Ca 2ϩ ] i during the following steps of mammalian development. Indirect evidence for a regulatory role was provided in experiments where development of mouse embryos was delayed by treatments with calcium channel blockers or with an inhibitor of calmodulin (20). It was only recently that [Ca 2ϩ ] i changes were studied directly during early mouse development. An endogenous increase in Ca 2ϩ -releasing activity leading to Ca 2ϩ transients was observed during the first mitotic division (21). Moreover, the rate of cavitation and cell division was either accelerated or delayed by experimentally induced elevation or reduction of [Ca 2ϩ ] i , respectively (22-24). Stacheki and Armant (24) suggested that in mouse embryos, inositol 1,4,5-trisphosphate-sensitive and ryanodinesensitive Ca 2ϩ stores exist at the morula stage and that calmodulin is involved in mediating the Ca 2ϩ signaling effects to its distal targets.Despite these temporal effects, no other developmental events have been examined so far under conditions where [Ca 2ϩ ] i has been manipulated and therefore t...

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“…Su et al confirmed that TRPV5 was highly expressed in the mouse blastocyst by microarray analysis [13], and we also verified its strong expression in blastocysts, whereas the level was undetectable in ES cells (data not shown). Ca 2+ is an essential signal for many cellular processes in embryogenesis, e.g., fertilization [14], cavitation [15,16], blastocoel formation [15] and blastocyst outgrowth [17][18][19]. Taken together, BSPRY-1 may be involved in cell differentiation and division in the early embryo via regulation of Ca 2+ influx with TPRV5, and it may be one reason why the activity level of the two isoforms of BSPRY is different in early embryo and ES cells.…”

Section: Discussionmentioning

confidence: 99%

B-box and SPRY Domain Containing Protein (BSPRY) is Associated with the Maintenance of Mouse Embryonic Stem Cell Pluripotency and Early Embryonic Development

Ikeda

Inoue

Ohkoshi

et al. 2012

Journal of Reproduction and Development

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Abstract. Mouse embryonic stem (ES) cells consist of heterogeneous populations with differing abilities to proliferate and differentiate. We previously demonstrated that the expression level of platelet endothelial cell adhesion molecule 1 (PECAM1)/CD31 was positively correlated with the undifferentiated state of mouse ES cells. In order to screen for a novel gene(s) involved in ES cell pluripotency, we performed an oligo microarray analysis and identified that B-box and SPRY domain containing protein (BSPRY) was expressed at high levels in PECAM1-positive cells. Two splice isoforms of BSPRY, BSPRY-1 and BSPRY-2, were expressed in undifferentiated ES cells and in blastocysts. Knockdown of BSPRY-1/2 in ES cells significantly reduced the number of undifferentiated colonies and caused increased expression of primitive ectoderm marker gene Fgf5. The overexpression of BSPRY-2 reciprocally increased the number of undifferentiated ES cells in the presence of LIF. Similarly, injection of BSPRY-1/2 siRNAs into 2-cell embryos caused developmental retardation and degeneration of embryos, and a significant decrease in the number of cells, especially in the inner cell mass (ICM), was observed at the blastocyst stage. Furthermore, microinjection of a BSPRY-1 expression vector into pronuclear stage embryos resulted in an increase in the hatching blastocysts rate after 120 h of culture. These results suggest that BSPRY-1 and BSPRY-2 are associated with both ES cell pluripotency and early embryonic development. E mbryonic stem (ES) cells are derived from the inner cell mass (ICM) of blastocysts and maintain the pluripotency to differentiate into three germ layers. Recent studies have demonstrated that mouse ES cells consist of heterogeneous populations with differing abilities to proliferate and differentiate [1][2][3]. We previously reported that there was wide variation in the expression of platelet endothelial cell adhesion molecule 1 (PECAM1) [1]. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis revealed that marker genes for undifferentiated ES cells were highly expressed in PECAM1-positive cells. PECAM1-positive ES cells injected into 8-cell host embryos could contribute to the epiblast in a chimeric embryo, but PECAM1-negative ES cells failed to differentiate into epiblast cells. These results suggested that the PECAM1 expression level was positively correlated with the undifferentiated state of ES cells.In order to search for a novel gene(s) involved in ES cell pluripotency, we analyzed gene expression profiles in PECAM1-positive and PECAM1-negative populations using an oligo microarray and isolated several genes that were expressed at high levels in PECAM1-positive cells. We focused upon one of these genes, which encodes mouse B-box and SPRY domain containing protein (BSPRY) protein, because in situ hybridization analysis revealed that Bspry was also expressed at the ICM of blastocysts [4]. These results suggest that BSPRY may be involved in not only ES cell pluripotency but also early emb...

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Mouse blastocyst outgrowth and implantation rates following exposure to ethanol or A23187 during culture in vitro

Cited by 54 publications

References 0 publications

Consequences of calcium decline on the embryogenesis and life history of Daphnia magna

Consequences of calcium decline on the embryogenesis and life history of Daphnia magna

Increase of intracellular Ca ²⁺ and relocation of E-cadherin during experimental decompaction of mouse embryos

B-box and SPRY Domain Containing Protein (BSPRY) is Associated with the Maintenance of Mouse Embryonic Stem Cell Pluripotency and Early Embryonic Development

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Mouse blastocyst outgrowth and implantation rates following exposure to ethanol or A23187 during culture in vitro

Cited by 54 publications

References 0 publications

Consequences of calcium decline on the embryogenesis and life history of Daphnia magna

Consequences of calcium decline on the embryogenesis and life history of Daphnia magna

Increase of intracellular Ca 2+ and relocation of E-cadherin during experimental decompaction of mouse embryos

B-box and SPRY Domain Containing Protein (BSPRY) is Associated with the Maintenance of Mouse Embryonic Stem Cell Pluripotency and Early Embryonic Development

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Increase of intracellular Ca ²⁺ and relocation of E-cadherin during experimental decompaction of mouse embryos