Mouse egg ribosomes: evidence for storage in lattices

Bachvarova, Rosemary F.; Leon, Victor De; Spiegelman, Irwin

doi:10.1242/dev.62.1.153

Cited by 12 publications

(1 citation statement)

References 31 publications

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“…A hallmark of preimplantation development is the degradation of maternal or maternal-effect proteins soon after fertilization . Compared to degradation, synthesis and accumulation of new embryonic proteins may be more difficult to appreciate because the subunits of the oocyte ribosomes are stored in lattice structures, which may influence the ribosomes’ translational ability . Indeed, oocytes that lacked one of the candidate structural components of the lattices, PADI6, underwent ∼50% of the total protein synthesis that is normally measured at the 2 cell stage .…”

Section: Resultssupporting

confidence: 77%

Proteomic Analysis of Mouse Oocytes Identifies PRMT7 as a Reprogramming Factor that Replaces SOX2 in the Induction of Pluripotent Stem Cells

et al. 2016

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The reprogramming process that leads to induced pluripotent stem cells (iPSCs) may benefit from adding oocyte factors to Yamanaka's reprogramming cocktail (OCT4, SOX2, KLF4, with or without MYC; OSK(M)). We previously searched for such facilitators of reprogramming (the reprogrammome) by applying label-free LC-MS/MS analysis to mouse oocytes, producing a catalog of 28 candidates that are (i) able to robustly access the cell nucleus and (ii) shared between mature mouse oocytes and pluripotent embryonic stem cells. In the present study, we hypothesized that our 28 reprogrammome candidates would also be (iii) abundant in mature oocytes, (iv) depleted after the oocyte-to-embryo transition, and (v) able to potentiate or replace the OSKM factors. Using LC-MS/MS and isotopic labeling methods, we found that the abundance profiles of the 28 proteins were below those of known oocyte-specific and housekeeping proteins. Of the 28 proteins, only arginine methyltransferase 7 (PRMT7) changed substantially during mouse embryogenesis and promoted the conversion of mouse fibroblasts into iPSCs. Specifically, PRMT7 replaced SOX2 in a factor-substitution assay, yielding iPSCs. These findings exemplify how proteomics can be used to prioritize the functional analysis of reprogrammome candidates. The LC-MS/MS data are available via ProteomeXchange with identifier PXD003093.

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Section: Resultssupporting

confidence: 77%

Proteomic Analysis of Mouse Oocytes Identifies PRMT7 as a Reprogramming Factor that Replaces SOX2 in the Induction of Pluripotent Stem Cells

et al. 2016

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Capacity of fertilized ova from mature and middle-aged rats to undergo preimplantation development in vitro

1983

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The ability of ova collected from mated middle-aged rats (9-10 months of age) to undergo preimplantation development in vitro was compared to that of younger controls (3-4 months of age). The majority of ova from young rats underwent at least one cleavage division for every 24 h of the 96 h culture period. Seventy-two percent reached the morula/blastocyst stage at the termination of the culture. Aged ova had a reduced capacity to undergo preimplantation development in vitro with the number of fragmented and/or degenerated ova increasing after each day in culture. At the end of the culture period, 81.6% of the ova had degenerated. It is concluded that middle-aged rats ovulate defective ova and that these defective ova account, in part, for the preimplantation losses that characterize middle-aged rats.

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Ultrastructure and morphometric analysis of preimplantation mouse embryos

Čech

Sedláčková

1983

Cell Tissue Res.

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During cleavage, both qualitative and quantitative morphologic characters of mouse ova change. Up to the 8-cell stage, the volume density of mitochondria remains nearly the same although it increases during early and late blastocyst stages. While a rise of the volume fraction of granular endoplasmic reticulum is noticed during cleavage, the volume density of agranular endoplasmic reticulum diminishes gradually from the 1-cell stage onwards. An increase in the volume fraction of autophagic vacuoles is found, the maximum being reached in early blastocysts. The volume fraction of crystalloid inclusions slightly increases after the 4-cell stage, but this increase is statistically insignificant. The volume density of filamentous material (plaques) conspicuously decreases in all studied embryos from the beginning of cleavage. Starting with the 2-cell stage, the volume fraction of lipid droplets remains practically unchanged. No differences in volume densities of the Golgi apparatus, multivesicular and residual bodies, and large cytoplasmic vesicles with medium electron-dense content are found between the respective cleavage stages.

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Mouse egg ribosomes: evidence for storage in lattices

Cited by 12 publications

References 31 publications

Proteomic Analysis of Mouse Oocytes Identifies PRMT7 as a Reprogramming Factor that Replaces SOX2 in the Induction of Pluripotent Stem Cells

Proteomic Analysis of Mouse Oocytes Identifies PRMT7 as a Reprogramming Factor that Replaces SOX2 in the Induction of Pluripotent Stem Cells

Capacity of fertilized ova from mature and middle-aged rats to undergo preimplantation development in vitro

Ultrastructure and morphometric analysis of preimplantation mouse embryos

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