Mouse fetal kidneys in serum-free organ culture: Effects of epidermal growth factor and hydrocortisone

Brière, Normand; Bertrand, L; Ferrari, J.

doi:10.1016/0300-9629(91)90426-d

Cited by 11 publications

(5 citation statements)

References 36 publications

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“…These findings suggest that newly expressed EGF in regenerated PT might contribute to PT maturation. This is also supported by in vitro study, in which EGF is proved to have regulatory effect on maturation of mouse fetal tubular brush border [35].…”

Section: Discussionsupporting

confidence: 56%

Relation of Distal Nephron Changes to Proximal Tubular Damage in Uranyl Acetate-Induced Acute Renal Failure in Rats

et al. 2002

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Aims: To elucidate the pathophysiological roles of the changes of distal nephron in uranyl acetate (UA)-induced acute renal failure (ARF), we investigated the relation of changes of constituent molecules in distal nephron to proximal tubular damage and repair in UA-treated rats. Methods: ARF was induced in rats by intravenous injection of UA, and all rats received bromodeoxyuridine (BrdU) intraperitoneally 1 h before sacrifice. Results: Proximal tubular damage with necrosis appeared as early as day 2, mainly in the outer stripe of outer medulla and reached a peak level at day 5. Slight cellular damage was evident in the distal nephron as early as day 3, reaching a peak level around day 9. Immunoreactive BrdU- or vimentin-positive regenerating proximal tubules (PT) appeared at day 2 and regenerating PT relining was almost completed by day 7. Immunostaining for EGF, which was constitutively expressed in the thick ascending limb (TAL) and distal convoluted tubule (DCT), diminished significantly as early as day 2, when PT regeneration became evident, and remained below normal levels until day 21. In contrast, slight immunoreactivity for EGF was observed in regenerated PT accompanying brush-border formation mainly after day 9, suggesting newly expressed EGF might contribute to PT maturation. Lectin staining or immunostaining for representative constituent molecules of the thin descending limb, TAL, DCT and collecting duct demonstrated marked and transient reduction after day 5. Conclusions: EGF was not associated with regenerating PT, but may be involved in the maturation of PT. Transient reduction in expression of constituent molecules of the distal nephron following the reduction in EGF could reflect dedifferentiation or phenotypic simplification during regenerative repair of PT in UA-induced ARF in rats.

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Section: Discussionsupporting

confidence: 56%

Relation of Distal Nephron Changes to Proximal Tubular Damage in Uranyl Acetate-Induced Acute Renal Failure in Rats

et al. 2002

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“…Furthermore, the murine cells were grown in medium with a low (0,021 mg/ ml) concentration of Ca-^, as compared with the 0.155 mg/ml in the present medium, Glucocorticoids, e.g, HC. influence the morphology and physiology of many organs and tissues [31] and induce a wide range ofmembranal and genomic effects on cells including Ihc cells ofthe immune system [32]. HChas been reported to have no effect on the proliferation of human or murine epithelial cells [9.…”

Section: Discussionmentioning

confidence: 99%

Effects of Growth Factors on Cytokine Production in Serum‐Free Cultures of Human Thymic Epithelial Cells

et al. 1993

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Human thymic epithelial cells (TEC) of medullary phenotype were cultured for 14 days in a growth factor-defined serum-free medium. The effects of added growth factors on cell numbers and the production of cytokines were investigated by separate exclusion of the various growth factors from the medium. We found that hydrocortisone stimulated cell proliferation but inhibited the differentiation of TEC and significantly reduced the production of interleukin-1 alpha, interleukin-6 and granulocyte-macrophage colony stimulating factor. Insulin was found to enhance the differentiation of TEC and the production of the three cytokines. Transferrin and choleratoxin were found to inhibit cell proliferation, but they did not affect production of the cytokines. Exclusion of epidermal growth factor, however, leads to cell death. We conclude that it is essential to exclude hydrocortisone from the medium to optimize production of cytokines, and that transferrin and choleratoxin seem to be unnecessary constituents in serum-free cultures of human TEC.

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“…In organ culture, addition of EGF to the medium accelerates DNA synthesis in the kidney of fetal mice [22]. An in vitro study showed that the pattern of DNA synthesis in the kidney parallels that of the specific binding of EGF to the renal cell membrane and that EGF stimulates hyperplastic growth in developing rat kidneys [10].…”

Section: Discussionmentioning

confidence: 99%

Perinatal Development of the Rat Kidney: Proliferative Activity and Epidermal Growth Factor

Okada

Iwamoto²,

Kusakabe³

et al. 2001

Neonatology

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The immunolocalization of proliferating cell nuclear antigen (PCNA), epidermal growth factor (EGF) and its receptor (EGFR) was examined in the perinatal rat kidney. As the index of proliferative activity, PCNA-positive cell ratios in glomeruli and proximal tubules were determined. The PCNA-positive ratios in both glomeruli and proximal tubules decreased significantly during the perinatal period and tended to decrease as the glomerular developmental stage proceeded as well. PCNA-positive cells were seen predominantly in the nephrogenic zone of the kidney throughout the period examined. They were noted in the collecting ducts of the nephrogenic zone and were rarely seen in those of the central zone of the kidney. On the other hand, PCNA-positive cells were noted in the straight portion of the proximal tubules and were rarely seen in the convoluted one. EGF-positive cells were seen in the proximal tubules, distal tubules and collecting ducts, though EGF-positive cells in the proximal tubules decreased after birth. EGFR-positive cells were seen along the entire length of the proximal tubules and collecting ducts as well as in immature glomeruli, not in mature ones. These results indicate that marked cell proliferation occurs in the collecting ducts in the peripheral area and in the proximal tubules in the central area of the kidney, that the proliferative activity decreases with age during the perinatal days and that EGF plays an important role in the proliferation of glomerular cells, and in both proliferation and maturation of the cells in the proximal tubules and collecting ducts.

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Mouse fetal kidneys in serum-free organ culture: Effects of epidermal growth factor and hydrocortisone

Cited by 11 publications

References 36 publications

Relation of Distal Nephron Changes to Proximal Tubular Damage in Uranyl Acetate-Induced Acute Renal Failure in Rats

Relation of Distal Nephron Changes to Proximal Tubular Damage in Uranyl Acetate-Induced Acute Renal Failure in Rats

Effects of Growth Factors on Cytokine Production in Serum‐Free Cultures of Human Thymic Epithelial Cells

Perinatal Development of the Rat Kidney: Proliferative Activity and Epidermal Growth Factor

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