Mouse Glutathione S-Transferase Ya Subunit: Gene Structure and Sequence

Daniel, Violet; Sharon, Ronit; Tichauer, Yehuda; Sarid, S

doi:10.1089/dna.1987.6.317

Cited by 54 publications

(42 citation statements)

References 33 publications

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“…The subunits of mammalian GSTs fall into three classes, Alpha, Mu and Pi, according to their primary structures (Mannervik et al, 1985;Pickett, 1987), and their gene structure is conserved between rat (Telakowski-Hopkins et al, 1986) and mouse (Daniel et al, 1987) for Alpha class genes and between rat (Okuda et al, 1987) and human (Cowell et al, 1988;Morrow et al, 1989) for the Pi class genes. For Mu class genes, three rat sequences have been compared and gene conversion (i.e.…”

Section: Introductionmentioning

confidence: 99%

Structure of human glutathione S-transferase class Mu genes

Taylor¹,

Oliver²,

Sherrington³

et al. 1991

Biochemical Journal

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Nucleotide sequencing of a human cosmid clone shows that the exon-intron structures of a glutathione S-transferase multigene family are conserved between man and rat, that the human gene family is clustered and that gene conversion events have occurred within the cluster. In addition, between man and rat, there is a high degree of nucleotide sequence identity not only in exons but also in some introns. These conserved sequences are coincident with homologous sequences subject to gene conversion in both species, and hence the utilization of gene conversion by this gene family has itself been conserved. By using transient-expression assay the conserved/converted regions are shown to be capable of modulating transcriptional activity. The data suggest that DNA repair by gene conversion may be a chemical immunity mechanism. which could result in acquired resistance to toxins and, in particular, drug resistance due to glutathione S-transferase in tumours.

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Section: Introductionmentioning

confidence: 99%

Structure of human glutathione S-transferase class Mu genes

Taylor¹,

Oliver²,

Sherrington³

et al. 1991

Biochemical Journal

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“…4). It is interesting that the 5' region of the human GST gene presented here shows no striking similarity in the analysed 5' region to the corresponding regions of the rat [11] and mouse [12,27] Alpha GST genes, although the NFl and the half ATTAAC motifs are also located at the 5' region of the mouse gene [27]. The SPI site was also found in the human GST Pi gene [14].…”

Section: Pgth2mentioning

confidence: 61%

“…1 and 2). Like its homologue in rat [11] and mouse [12] the human GST Alpha gene contains seven exons (El-E7) interrupted by six introns. Furthermore, clone cosGTH2 contains additional regions corresponding to exons 3 and 4 (E3+ and E4+) ( Figs.…”

Section: Pgth2mentioning

confidence: 99%

“…These two genes are represented by two different cDNAs, namely GSTal2k [5], pGTH1 [8] or AGST2-3 [9] on the one hand and pGTH2 [10,12] on the other. The existence of two genes correlates with the fact that two signals of 0.8 kb and 1.0 kb are detected on Northernblot analysis [5].…”

Section: Pgth2mentioning

confidence: 99%

“…So far, one Alpha GST gene of rat [1 1] and of mouse [12] have been described, and their gene structure has been shown to be highly conserved [8,10]. Regions of human GST loci have been isolated for the Mu [13] and Pi genes [14] but so far not for the Alpha GST genes.…”

Section: Introductionmentioning

confidence: 99%

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Cloning, sequencing and characterization of the human Alpha glutathione S-transferase gene corresponding to the cDNA clone pGTH2

Klöne

Hussnätter

Sies

1992

Biochemical Journal

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The human Alpha glutathione S-transferase gene corresponding to the human liver cDNA clone pGTH2 was isolated from a cosmid genome library. The gene, represented by the clone cosGTH2, spans nearly 12 kb and contains seven exons. The intron/exon borders conform to the standard rules, and an open reading frame is present, starting at position 67 in exon 2, the double-stop codon being at position 733 in exon 7. Exons 1, 2 and 7 differ in length from the known rat gene coding for the Ya enzyme. A 209 bp 5'-upstream region contains TATA and CAT boxes and, in addition, motifs for Spl-, NFl-and HNFI-binding factors. Clone cosGTH2 represents the less basic subunit, ay, of two Alpha glutathione S-transferase subunits (ax and ac) expressed in liver, which is identical with the kidney subunit a2

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Patterns of gene expression along the crypt-villus axis in mouse jejunal epithelium

Cheng

Bjerknes

1996

Anat. Rec.

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Background There is considerable interest in gene expression along the crypt‐villus axis of the small intestinal epithelium, particularly in the identification of genes expressed in intestinal crypts. Methods In an attempt to identify crypt‐expressed genes, single‐stranded cDNA made from normal mouse jejunal epithelium was used in subtractive hybridization against single‐stranded cDNA from epithelium from which crypt cells were depleted by 2,000 rads of gamma irradiation. Partial DNA sequence and in situ hybridization of 72 resulting clones were determined. Results The sequence of 45 clones matched previously published genes. Gene expression patterns fell into three categories: expression throughout the crypt‐villus axis, expression restricted to the villus, and expression restricted to the crypt. Clones in the first two categories could be further divided into three subgroups: those with uniform expression, those with an increasing gradient of expression, and those with a decreasing gradient of expression along the crypt‐villus axis. Twenty two clones showed a stronger expression in crypt and lower villus cells, four of these were differentially localized to the crypt. Two of the crypt localized clones were uniformly expressed throughout the crypt, expression of one was stronger in the lower crypt, and expression of the remaining clone was enhanced Paneth cells. We report the full‐length cDNA sequence of the Paneth‐cell‐enhanced clone. Conclusions The screen isolated crypt‐expressed genes that may prove useful tools in the study of crypt biology. In a companion report, we characterize one of the crypt clones. © 1996 Wiley‐Liss, Inc.

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Mouse Glutathione S-Transferase Ya Subunit: Gene Structure and Sequence

Cited by 54 publications

References 33 publications

Structure of human glutathione S-transferase class Mu genes

Structure of human glutathione S-transferase class Mu genes

Cloning, sequencing and characterization of the human Alpha glutathione S-transferase gene corresponding to the cDNA clone pGTH2

Patterns of gene expression along the crypt-villus axis in mouse jejunal epithelium

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