Mouse models of Ebola virus tolerance and lethality: characterization of CD-1 mice infected with wild-type, guinea pig-adapted, or mouse-adapted virus

Spengler, Jessica R.; Welch, Stephen R.; Ritter, Jana M.; Harmon, Jessica R.; Coleman-McCray, JoAnn D.; Genzer, Sarah C.; Seixas, Josilene Nascimento; Scholte, Florine E. M.; Davies, Katherine A; Bradfute, Steven B.; Spiropoulou, Christina F.

doi:10.1016/j.antiviral.2022.105496

Cited by 3 publications

(1 citation statement)

References 44 publications

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“…This strain is preferred as an animal model in the study of many diseases such as cancer (Roby et al., 2000), diabetes (Breyer et al., 2005), Alzheimer (Hofrichter et al., 2016), asthma (Maud et al., 2009), insulin resistance (Fraulob et al., 2010) and atherosclerosis (Fazio & Linton, 2001). Balb‐c mouse, another inbred strain; asthma (Maud et al., 2009), vaccine studies, (Zhou et al., 2009; Jiang et al., 2017; Gu et al., 2020), immunological studies, (Dormans et al., 2004; Kong et al., 2005; Schöll et al., 2004), toxicology studies (Pirela et al., 2013; Rahman et al., 2014) and CD 1 group of mouse are used as models especially in viral infection studies (Horie et al., 1999; Spengler et al., 2023), tumour studies (Morscher et al., 2015) and behavioural studies (Yang et al., 2004).…”

Section: Introductionmentioning

confidence: 99%

Shape variation in cranium, mandible and teeth in selected mouse strains

Ağaç,

Oktay,

Onuk

et al. 2024

Anat Histol Embryol

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There are different strains of laboratory mouse used in many different fields. These strains differ anatomically. In order to determine these anatomical differences, shape analysis was conducted according to species. CD‐1, C57bl/6 and Balb‐c strains were preferred to study these differences. Forty‐eight adult mouse strains belonging to these strains were utilized. The bones were photographed and geometric morphometry was applied to these photographs. Principal Component Analysis was applied to determine shape variations. In Principal component 1 for cranium, CD‐1 and C57bl/6 strain groups showed different shape variations, while Balb‐c strain group showed similar shape variations to the other strain groups. Principal Component 1 for the mandible separated the CD‐1 and C57bl/6 strain groups in terms of shape variation. Principal Component 2 explained most of the variation between the C57bl/6 and CD‐1 lineage groups. In PC1 for molars, the CD‐1 group showed a different shape variation from the other groups. Mahalanobis distances and Procrustes distances were measured using Canonical variance analysis to explain the differences between the lineage groups. These measurements were statistically significant. For cranium, in canonical variate 1, CD‐1 group of mouse and Balb‐c group of mouse were separated from each other. In canonical variate 2, C57bl/6 group of mouse were separated from the other groups. For mandible, Balb‐c group of mouse in canonical variate 1 and CD‐1 group of mouse in canonical variate 2 were separated from the other groups. For molars, CD‐1 group of mouse in canonical variate 1 and Balb‐c group of mouse in canonical variate 2 were separated from the other groups. It was thought that these anatomical differences could be caused by genotypic factors as well as dietary differences and many different habits that would affect the way their muscles work.

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Section: Introductionmentioning

confidence: 99%

Shape variation in cranium, mandible and teeth in selected mouse strains

Ağaç,

Oktay,

Onuk

et al. 2024

Anat Histol Embryol

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Fluorescent and Bioluminescent Reporter Mouse-Adapted Ebola Viruses Maintain Pathogenicity and Can Be Visualized in Vivo

Davies

Welch

Jain

et al. 2023

The Journal of Infectious Diseases

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Ebola virus (EBOV) causes lethal disease in humans but not in mice. Here, we generated recombinant mouse-adapted (MA)-EBOVs, including one based on the previously reported serially adapted strain (rMA-EBOV), along with single-reporter rMA-EBOVs expressing either fluorescent (ZsGreen1 [ZsG]) or bioluminescent (nano-luciferase [nLuc]) reporters, and dual-reporter rMA-EBOVs expressing both ZsG and nLuc. No detriment to viral growth in vitro was seen with inclusion of MA-associated mutations or reporter proteins. In CD-1 mice, infection with MA-EBOV, rMA-EBOV, and single-reporter rMA-EBOVs conferred 100% lethality; infection with dual-reporter rMA-EBOV resulted in 80% lethality. Bioluminescent signal from rMA-EBOV expressing nLuc was detected in vivo and ex vivo using the IVIS Spectrum CT. Fluorescent signal from rMA-EBOV expressing ZsG was detected in situ using hand-held blue-light transillumination and ex vivo through epi-illumination with the IVIS Spectrum CT. These data support the use of reporter MA-EBOV for studies of Ebola virus in animal disease models.

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Filoviruses: Scientific Gaps and Prototype Pathogen Recommendation

Dupuy,

Spiropoulou,

Towner

et al. 2023

The Journal of Infectious Diseases

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Viruses in the family Filoviridae, including the commonly known Ebola (EBOV) and Marburg (MARV) viruses, can cause severe hemorrhagic fever in humans and nonhuman primates. Sporadic outbreaks of filovirus disease occur in sub-Saharan Africa with reported case fatality rates ranging from 25% to 90%. The high mortality and increasing frequency and magnitude of recent outbreaks along with the increased potential for spread from rural to urban areas highlight the importance of pandemic preparedness for these viruses. Despite their designation as high-priority pathogens, numerous scientific gaps exist in critical areas. In this review, these gaps and an assessment of potential prototype pathogen candidates are presented for this important virus family.

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Mouse models of Ebola virus tolerance and lethality: characterization of CD-1 mice infected with wild-type, guinea pig-adapted, or mouse-adapted virus

Cited by 3 publications

References 44 publications

Shape variation in cranium, mandible and teeth in selected mouse strains

Shape variation in cranium, mandible and teeth in selected mouse strains

Fluorescent and Bioluminescent Reporter Mouse-Adapted Ebola Viruses Maintain Pathogenicity and Can Be Visualized in Vivo

Filoviruses: Scientific Gaps and Prototype Pathogen Recommendation

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