Mouse Models of Erythropoiesis and Associated Diseases

Parker, Matthew P.; Peterson, Kenneth R.

doi:10.1007/978-1-4939-7428-3_3

Cited by 9 publications

(6 citation statements)

References 111 publications

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“…While the physiology is mostly comparable between different strains of mice, some differences are noted due to variability in the genetic background. [38][39][40][41] We focused on C57BL/6 due to its wide use in red cell biology, but this study will need to be adapted to the background (pure or mixed) as well as the genetically modified strain used.…”

Section: Discussionmentioning

confidence: 99%

Dynamic changes in murine erythropoiesis from birth to adulthood: implications for the study of murine models of anemia

et al. 2020

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Liver, spleen, and bone marrow are 3 key erythropoietic tissues in mammals. In the mouse, the liver is the predominant site of erythropoiesis during fetal development, the spleen responds to stress erythropoiesis, and the bone marrow is involved in maintaining homeostatic erythropoiesis in adults. However, the dynamic changes and respective contributions of the erythropoietic activity of these tissues from birth to adulthood are incompletely defined. Using C57BL/6 mice, we systematically examined the age-dependent changes in liver, spleen, and bone marrow erythropoiesis following birth. In addition to bone marrow, the liver and spleen of newborn mice sustain an active erythropoietic activity that is gradually lost during first few weeks of life. While the erythropoietic activity of the liver is lost 1 week after birth, that of the spleen is maintained for 7 weeks until the erythropoietic activity of the bone marrow is sufficient to sustain steady-state adult erythropoiesis. Measurement of the red cell parameters demonstrates that these postnatal dynamic changes are reflected by varying indices of circulating red cells. While the red cell numbers, hemoglobin concentration, and hematocrit progressively increase after birth and reach steady-state levels by week 7, reticulocyte counts decrease during this time period. Mean cell volume and mean cell hemoglobin progressively decrease and reach steady state by week 3. Our findings provide comprehensive insights into developmental changes of murine erythropoiesis postnatally and have significant implications for the appropriate interpretation of findings from the variety of murine models used in the study of normal and disordered erythropoiesis.

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Section: Discussionmentioning

confidence: 99%

Dynamic changes in murine erythropoiesis from birth to adulthood: implications for the study of murine models of anemia

et al. 2020

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“…From the assembly of the CRISPR/Cas9 complex and the discovery of direct targeting of specific genomic sequences in vitro 9, 27 , it took only six months to experimentally verify in vitro findings in bacterial and mammalian cells 3,4,28 . The establishment of genetically modified mouse models to study the potential functional roles of genes and their products in human diseases is an important aspect of biomedical studies [29][30][31][32][33] .…”

Section: Discussionmentioning

confidence: 99%

Pervasive head-to-tail insertions of DNA templates mask desired CRISPR/Cas9-mediated genome editing events

Skryabin

Gubar

Seeger

et al. 2019

Preprint

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CRISPR/Cas9 mediated homology-directed DNA repair is the method of choice for precise gene editing in a wide range of model organisms, including mouse and human.Broad use by the biomedical community refined the method, making it more efficient and sequence specific. Nevertheless, the rapidly evolving technique still contains pitfalls. During the generation of six different conditional knock-out mouse models, we discovered that frequently (sometimes solely) homology-directed repair and/or non-homologous end-joining mechanisms caused multiple unwanted head-to-tail

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“…Shortcomings of Existing Animal Models. Rodents are the most widely used experimental animal models (more than 90%) [65][66][67][68][69][70][71][72][73]. Although the mouse model is low cost and easy to operate, it has limitations in revealing human biology.…”

Section: Challenges and Opportunities Of Existing Animal Modelsmentioning

confidence: 99%

Embryo-Engineered Nonhuman Primate Models: Progress and Gap to Translational Medicine

2021

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Animal models of human diseases are vital in better understanding the mechanism of pathogenesis and essential for evaluating and validating potential therapeutic interventions. As close relatives of humans, nonhuman primates (NHPs) play an increasingly indispensable role in advancing translational medicine research. In this review, we summarized the progress of NHP models generated by embryo engineering, analyzed their unique advantages in mimicking clinical patients, and discussed the remaining gap between basic research of NHP models to translational medicine.

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Mouse Models of Erythropoiesis and Associated Diseases

Cited by 9 publications

References 111 publications

Dynamic changes in murine erythropoiesis from birth to adulthood: implications for the study of murine models of anemia

Dynamic changes in murine erythropoiesis from birth to adulthood: implications for the study of murine models of anemia

Pervasive head-to-tail insertions of DNA templates mask desired CRISPR/Cas9-mediated genome editing events

Embryo-Engineered Nonhuman Primate Models: Progress and Gap to Translational Medicine

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