Mouse Neural Stem Cell Differentiation and Human Adipose Mesenchymal Stem Cell Transdifferentiation Into Neuron- and Oligodendrocyte-like Cells With Myelination Potential

Santos, Anderson K.; Gomes, Kátia N.; Parreira, Ricardo Cambraia; Scalzo, Sérgio; Pinto, Mauro Cunha Xavier; Santiago, Helton C; Birbrair, Alexander; Sack, Ulrich; Ulrich, Henning; Resende, Rodrigo R.

doi:10.1007/s12015-021-10218-7

Cited by 13 publications

(6 citation statements)

References 95 publications

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“…In particular, better outcomes have been reported when transplanted MSCs were previously induced toward a neural differentiation [5]. Studies have shown that MSCs can be horizontally differentiated into non-mesoderm derived cells, such as glial cells and neurons [6,7], which provide a new strategy for the clinical treatment of neurodegenerative diseases.…”

Section: Introductionmentioning

confidence: 99%

Wnt/PCP pathway regulates the migration and neural differentiation of mesenchymal stem cells in vitro

Yao

Zhu

et al. 2022

Folia Histochem Cytobiol.

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Section: Introductionmentioning

confidence: 99%

Wnt/PCP pathway regulates the migration and neural differentiation of mesenchymal stem cells in vitro

Yao

Zhu

et al. 2022

Folia Histochem Cytobiol.

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“… 3 In this study, NSCs exhibited a self-renewal ability, which resulted in their aggregation into clusters of cells 20 that differentiated into neurons, oligodendrocytes, and astrocytes under optimal in vitro inducing conditions. 21 Therefore, the transplantation of exogenous NSCs could allow for the replacement of degenerated neurons and the regeneration of injured CNSs. 4 However, the collection of NSCs from the SVZ and SGZ of adult brains was shown to cause donor site morbidity, harming donors and creating ethical concerns.…”

Section: Discussionmentioning

confidence: 99%

Characterization of neural stem cells derived from human stem cells from the apical papilla undergoing three-dimensional neurosphere induction

SONGSAAD,

THAIRAT,

SEEMAUNG

et al. 2023

J. Appl. Oral Sci.

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Objectives The endogenous repairing based on the activation of neural stem cells (NSCs) is impaired by neurodegenerative diseases. The present study aims to characterize human stem cells from the apical papilla (hSCAPs) with features of mesenchymal stem cells (MSCs) and to demonstrate the neuronal differentiation of hSCAPs into NSCs through the formation of three-dimensional (3D) neurospheres, verifying the structural, immunophenotyping, self-renewal, gene expression and neuronal activities of these cells to help further improve NSCs transplantation. Methodology The hSCAPs were isolated from healthy impacted human third molar teeth and characterized as MSCs. They were then induced into 3D-neurospheres using a specific neural induction medium. Subsequently, the intra-neurospheral cells were confirmed to be NSCs by the identification of Nissl substance and the analysis of immunofluorescence staining, self-renewal ability, and gene expression of the cells. Moreover, the neuronal activity was investigated using intracellular calcium oscillation. Results The isolated cells from the human apical papilla expressed many markers of MSCs, such as self-renewal ability and multilineage differentiation. These cells were thus characterized as MSCs, specifically as hSCAPs. The neurospheres induced from hSCAPs exhibited a 3D-floating spheroidal shape and larger neurospheres, and consisted of a heterogeneous population of intra-neurospheral cells. Further investigation showed that these intra-neurospheral cells had Nissl body staining and also expressed both Nestin and SOX2. They presented a self-renewal ability as well, which was observed after their disaggregation. Their gene expression profiling also exhibited a significant amount of NSC markers ( NES , SOX1 , and PAX6 ). Lastly, a large and dynamic change of the fluorescent signal that indicated calcium ions (Ca 2+ ) was detected in the intracellular calcium oscillation, which indicated the neuronal activity of NSCs-derived hSCAPs. Conclusions The hSCAPs exhibited properties of MSCs and could differentiate into NSCs under 3D-neurosphere generation. The present findings suggest that NSCs-derived hSCAPs may be used as an alternative candidates for cell-based therapy, which uses stem cell transplantation to further treat neurodegenerative diseases.

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“…Samples were stained for Ki-67, a nuclear proliferation marker, [82] as well as for 𝛽 III-tubulin and MAP2, an early and a mature neuronal marker, respectively. [83][84][85] The overall expression of these proteins indicates that the differentiation of the neurospheres embedded in the CE_CND1 matrix is promoted, showing significantly lower amounts of Ki-67-positive cells and higher levels of 𝛽 III-tubulin and MAP2 (Figure 5B). These findings are also corroborated by the relative expression of calretinin (an early neuronal marker) and nestin (a NP marker).…”

Section: Neuronal Differentiationmentioning

confidence: 99%

Electroconductive Collagen‐Carbon Nanodots Nanocomposite Elicits Neurite Outgrowth, Supports Neurogenic Differentiation and Accelerates Electrophysiological Maturation of Neural Progenitor Spheroids

Lomboni,

Ozgun,

de Medeiros

et al. 2023

Adv Healthcare Materials

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Neuronal disorders are characterized by the loss of functional neurons and disrupted neuroanatomical connectivity, severely impacting the quality of life of patients. This study investigates a novel electroconductive nanocomposite consisting of glycine‐derived carbon nanodots (GlyCNDs) incorporated into a collagen matrix and validates its beneficial physicochemical and electro‐active cueing to relevant cells. To this end, this work employs mouse induced pluripotent stem cell (iPSC)‐derived neural progenitor (NP) spheroids. The findings reveal that the nanocomposite markedly augmented neuronal differentiation in NP spheroids and stimulate neuritogenesis. In addition, this work demonstrates that the biomaterial‐driven enhancements of the cellular response ultimately contribute to the development of highly integrated and functional neural networks. Lastly, acute dizocilpine (MK‐801) treatment provides new evidence for a direct interaction between collagen‐bound GlyCNDs and postsynaptic N‐methyl‐D‐aspartate (NMDA) receptors, thereby suggesting a potential mechanism underlying the observed cellular events. In summary, the findings establish a foundation for the development of a new nanocomposite resulting from the integration of carbon nanomaterials within a clinically approved hydrogel, toward an effective biomaterial‐based strategy for addressing neuronal disorders by restoring damaged/lost neurons and supporting the reestablishment of neuroanatomical connectivity.

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Mouse Neural Stem Cell Differentiation and Human Adipose Mesenchymal Stem Cell Transdifferentiation Into Neuron- and Oligodendrocyte-like Cells With Myelination Potential

Cited by 13 publications

References 95 publications

Wnt/PCP pathway regulates the migration and neural differentiation of mesenchymal stem cells in vitro

Wnt/PCP pathway regulates the migration and neural differentiation of mesenchymal stem cells in vitro

Characterization of neural stem cells derived from human stem cells from the apical papilla undergoing three-dimensional neurosphere induction

Electroconductive Collagen‐Carbon Nanodots Nanocomposite Elicits Neurite Outgrowth, Supports Neurogenic Differentiation and Accelerates Electrophysiological Maturation of Neural Progenitor Spheroids

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