Mouse-protective Properties of Bordetella pertussis Serotypes in Passive Tests

Abstract

In a passive protection procedure in which the ED50 values of Bordetellc/ pertuessis antisera were determined, groups of mice were given graded intraperitoneal doses of serum, followed the next day by intracerebral challenge with 100,000 organisms. Antiserum produced with B. pertussis culture 5373, serotype 1 3, protected mice against challenge with culture 18-323, serotype 1 2. 3, as effectively as did an antiserum produced with a serotype 1 2.3 culture. When two groups of mice similarly treated with pertussis immune serum were challenged with culture 353Z (serotype 1) and 18-323, respectively, much lower ED50 values were obtained with the animals challenged with 353Z. Passive protection tests with adsorbed antiserum gave equivocal results, suggesting that some of the adsorbing antigen remained in the serum and interfered with the tests. There was no evidence that serotype is related to protection.

Antigens of Bordetella pertussis V. Separation of Agglutinogen 1 and Mouse-Protective Antigen

“…However, Eldering et al (7,8) were not able to detect a significant difference in active or passive immunization studies with strains of different serotypes, and we, in the present report, could not show mouse protection with agglutinogen 1. However, these observations do not explain the role of these agglutinogen factors in the prophylaxis of whooping cough.…”

Desenvolvimento da produção industrial de uma nova Vacina Pertussis de células inteiras com baixa reatogenicidade.

“…Camundongos imunizados com fímbrias purificadas sobrevivem ao desafio contra a B. pertussis virulenta em modelo aerosol (Oda et al, 1985) e intracerebral (Eldering et al, 1967;Eldering et al, 1969). Estes dados levaram à inclusão de Fim2 e Fim 3 como antígenos em algumas novas formulações de vacinas acelulares (Locht, 1999).…”