Human Motion is the most effective and fundamental functionality utilizing the lower extremities or legs. The replication of human movement is possible in the humanoid robots with simulation related prerequisites. The forward and backward motion simulation of the normal left and right leg is the primary requirement which has been demonstrated in this research paper. The balancing of body is feasible with the leg motion simulation in different conditions such as vertical acceleration and horizontal velocity. The leg motion video of 8secs with forward and backward movement has been taken into consideration for the simulation purpose to check the activity level of the legs. The similar movements can be mimicked in the autonomous bio-robotic systems. Keywords: Leg movement, Robotics, Kinovea, Motion simulation.