Blue Catfish Ictalurus furcatus provide a popular sport fishery in Mark Twain Lake, Missouri, but little is known about their population demographics or exploitation by anglers. Our objectives were to (1) determine population demographics of Blue Catfish, (2) determine exploitation of Blue Catfish, and (3) determine whether new harvest regulations would improve the quality (e.g., size structure and abundance) of the fishery in Mark Twain Lake. We sampled Blue Catfish by using jug lines baited with pieces of Gizzard Shad Dorosoma cepedianum, the gear used by the majority of anglers in this reservoir. Blue Catfish grew slowly, reaching a preferred size (762 mm TL) in about 12 years. Annual mortality was estimated at 24.8% using a weighted catch curve. Using reward tags, we estimated exploitation to be between 8% and 12%, and we modeled the effects of 508‐, 610‐, and 660‐mm minimum TL limits on yield and size structure of the Blue Catfish population relative to a baseline of 381 mm TL, the smallest fish usually harvested by anglers. All three length limits increased the proportion of larger fish (≥762 mm TL) in the population without reducing yield, except for slight decreases (6% and 10%) under the 610‐ and 660‐mm limits at the highest modeled natural mortality rate. We recommend the 610‐mm limit instead of the 660‐mm limit because it allows more harvest and higher yields with increases in natural mortality and still doubles the proportion of larger fish relative to the 508‐mm limit.