Movement of internalized ligand–receptor complexes along a continuous endosomal reticulum

Hopkins, Colin R.; Gibson, Adele; Shipman, M.; Miller, Karen

doi:10.1038/346335a0

Cited by 413 publications

(250 citation statements)

References 23 publications

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“…These tubules have no attached ribosomes, but they are in continuity with the rough endoplasmic reticulum (32). The extensive network of tubules and vesicles that transport the virions from one part of the cell to another is also consistent with recently described cisternal compartments within intracellular transport pathways that include branching tubular elements that can be several micrometers long (33). Specifically regarding SV40 virions, it has been demonstrated that, while avoiding lysosomal degradation, they take advantage of a retrograde endocytic pathway that leads to the RER and from there to the nucleus.…”

Section: Discussionsupporting

confidence: 84%

BK Polyoma Virus Allograft Nephropathy: Ultrastructural Features from Viral Cell Entry to Lysis

Drachenberg¹,

Papadimitriou²,

Wali³

et al. 2003

American Journal of Transplantation

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BK virions must enter the host cell and target their genome to the nucleus in order to complete their life cycle. The mechanisms by which the virions accomplish these tasks are not known. In this morphological study we found that BK virions localized beneath the host cell cytoplasmic membrane in 60-70-nm, smooth (non-coated) monopinocytotic vesicles similar to, or consistent with, caveolae. In the cytoplasm, the monopinocytotic vesicles carrying virions appeared to fuse with a system of smooth, vesicles and tubules that communicated with the rough endoplasmic reticulum and was continuous with the Golgi system. Membrane-bound single virions and large tubuloreticular complexes loaded with virions accumulated in paranuclear locations. Occasional nuclei displayed virions within the perinuclear cisterna in association to the perinuclear viral accumulations. Tubular cells with mature productive infection had large nuclei, distended by daughter virions, whereas they lacked significant numbers of cytoplasmic virions. In addition to virally induced cell necrosis, there was extensive tubular cell damage (apoptosis and necrosis) in morphologically non-infected tubules. The observed ultrastructural interactions between the BK virions and host cells are remarkably similar to viral cell entry and nuclear targeting described for SV40 virus.

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Section: Discussionsupporting

confidence: 84%

BK Polyoma Virus Allograft Nephropathy: Ultrastructural Features from Viral Cell Entry to Lysis

Drachenberg¹,

Papadimitriou²,

Wali³

et al. 2003

American Journal of Transplantation

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“…Under the conditions of most rapid quenching of intracellular acidic pH, i.e. bafilomycin A1 plus monensin, cells are protected for more than 1 h. Cell ligands such as EGF or LDL enter early endosomal compartments within few minutes and move to late endosomal compartments within 20-30 min [27,28]. Based on these data, the present results indicate that LF undergoes the low pH-dependent step of its cell intoxication process in late endosomal compartments.…”

Section: Resultssupporting

confidence: 55%

The vacuolar ATPase proton pump is required for the cytotoxicity of Bacillus anthracis lethal toxin

et al. 1996

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The nature of the cytopathic effect exerted by the lethal factor toxin (LF) of Bacillus anthracis on sensitive cells is unknown. The toxin requires the passage through acidic vesicles in order to exert its effect within the cytosol. Here, we show that bafilomycins and concanamycin A, selective inhibitors of the vacuolar ATPase proton pump, are the most powerful known inhibitors of LF macrophage toxicity. These inhibitors are fully active long after LF addition to macrophages, suggesting that LF enters the cytosol after having reached a late endosomal compartment.

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“…Secondly, the early endosome network is not morphologically well characterized. Those studies that have been performed indicate that what is commonly referred to as the early endosome is in fact highly complex and heterogeneous and dependent on cell type (Marsh et al, 1986;Hopkins et al, 1990;Tooze and Hollinshead, 1991). In most cell types, this early endosome network is composed of at least two separate compartments, the sorting endosome and the recycling compartment, which are morphologically and functionally distinct (Dunn et al, 1989;Presley et al, 1993;Marsh et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

Biogenesis of Salmonella typhimurium-containing vacuoles in epithelial cells involves interactions with the early endocytic pathway

et al. 1999

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SummaryIn epithelial cells, the intracellular pathogen Salmonella typhimurium resides and replicates within a unique cytoplasmic organelle, the Salmonella-containing vacuole (SCV). In vitro studies have shown that the SCV is a dynamic organelle that selectively acquires lysosomal glycoproteins (lgps) without fusing directly with lyosomes. Here, we have investigated early events in SCV biogenesis using immuno¯uorescence microscopy and epitope-speci®c¯ow cytometry. We show that proteins speci®c to the early endocytic pathway, EEA1 and transferrin receptor (TR), are present on early SCVs. The association of these proteins with SCVs is transient, and both proteins are undetectable at later time points when lgp and vATPase are acquired. Analysis of the fraction of SCVs containing both TR and lamp-1 showed that TR is lost from SCVs as the lgp is acquired, and that these processes occur progressively and not as the result of a single fusion/ ®ssion event. These experiments reveal a novel mechanism of SCV biogenesis, involving previously undetected initial interactions with the early endocytic pathway followed by the sequential delivery of lgp. The pathway does not involve interactions with the late endosome/prelysosome and is distinct from traditional phagocytic and endocytic pathways. Our study indicates that intracellular S. typhimurium occupies a unique niche, branching away from the traditional endocytic pathway between the early and late endosomal compartments.

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Movement of internalized ligand–receptor complexes along a continuous endosomal reticulum

Cited by 413 publications

References 23 publications

BK Polyoma Virus Allograft Nephropathy: Ultrastructural Features from Viral Cell Entry to Lysis

BK Polyoma Virus Allograft Nephropathy: Ultrastructural Features from Viral Cell Entry to Lysis

The vacuolar ATPase proton pump is required for the cytotoxicity of Bacillus anthracis lethal toxin

Biogenesis of Salmonella typhimurium-containing vacuoles in epithelial cells involves interactions with the early endocytic pathway

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