Movements of radioactive potassium in isolated rat ganglia

Scholfield, C.N.

doi:10.1113/jphysiol.1977.sp011850

Cited by 6 publications

(1 citation statement)

References 13 publications

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“…The resting rate coefficient for 86Rb+-efflux into normal Krebs solution attained a 'basal' value of between 0.01 and 0.02 min-after 60 min or so, in agreement with previous observations on this tissue using both 86Rb+ and 42K' (Scholfield, 1977a). Reduction or elevation of external [K+] over the range 2 to 60 mm reduced or increased the efflux rate coefficient respectively (Figure 12a).…”

Section: Rb+-effluxsupporting

confidence: 90%

Muscarinic Receptors in Rat Sympathetic Ganglia

Brown

Fatherazi

Garthwaite

et al. 1980

British J Pharmacology

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I Potential changes in isolated superior cervical ganglia of the rat produced by muscarinic-receptor agonists were recorded by an extracellular 'air-gap' method. Depolarization was accompanied by facilitation of submaximal ganglionic transmission. 6 Muscarine (1 to 100 gM) initially reduced, then increased, the rate of 86Rb+-efflux from isolated ganglia at both 6 and 120 mM [K ]. These effects were reduced by 1 AM hyoscine.7 No consistent change in the amounts of cyclic 3',5'-guanosine monophosphate in isolated ganglia accompanying muscarinic depolarization could be detected.8 Mean against ED5o values (pM) for contracting the rat isolated ileum were: oxotremorine 0.012, methylfurmethide 0.29, (±)muscarine 0.48, pilocarpine 7.8 and AHR-602 9.9. Mean antagonist K, values (nM) were: hyoscine 0.17, atropine 0.34 and lachesine 0.27. 9 It is concluded that ganglionic muscarinic receptors are quite similar to ileal receptors in terms of agonist ED50 and antagonist K, values, and that the major difference between them lies in the greater 'efficacy' of certain agonists (pilocarpine, AHR-602 and McN-A-343) on the ganglion.

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Section: Rb+-effluxsupporting

confidence: 90%

Muscarinic Receptors in Rat Sympathetic Ganglia

Brown

Fatherazi

Garthwaite

et al. 1980

British J Pharmacology

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Intracellular electrolyte concentrations in rat sympathetic neurones measured with an electron microprobe

Galvan

Dörge²,

Beck

et al. 1984

Pflugers Arch.

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Intracellular element concentrations were measured in rat sympathetic neurones using energy dispersive electron microprobe analysis. The resting intracellular concentrations of sodium potassium and chloride measured in ganglia maintained for about 90 min in vitro at 25 degrees C were 3, 155 and 25 mmol/kg total tissue wet weight respectively. Recalculated in mmol/l cell water, these values are 5, 196 and 32 respectively. There were no significant differences between the nuclear and cytoplasmic values of these ions. Incubation in either carbachol (180 mumol/l, 4 min) or ouabain (1 mmol/1, 60 min) significantly increased the intracellular sodium and decreased the intracellular potassium concentrations. Neither substance materially altered the intracellular chloride concentration. The data obtained are compared and contrasted to those obtained in mammalian sympathetic neurones using chemical analysis and ion-sensitive microelectrodes.

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