Moxibustion inhibits interleukin-12 and tumor necrosis factor alpha and modulates intestinal flora in rat with ulcerative colitis

Wang, Xiaomei; Lei, Yuan; Wu, Luyi; Yu, Shu-Guang; Zhao, Bai-Xiao; Hu, Hongyi; Wu, Huangan; Bao, Chunhui; Liu, Huirong; Wang, Jinhai; Yao, Yi; Hua, Xue-gui; Huiying, Guo; Shen, Lirong

doi:10.3748/wjg.v18.i46.6819

Cited by 61 publications

(29 citation statements)

References 40 publications

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“…Similarly, another category of cytokines secreted from macrophages, the IL-1β, is involved in pro-inflammatory regulations that facilitates UC progression ( 48 ). In addition, T and natural killer cells are mediated by the IL-12 cytokine, which in turn fuels TNF-α secretion ( 49 ). As reported previously, pro-inflammatory cytokine levels (TNF-α, IL-12, and IL-1β) in colonic tissues were elevated significantly by DSS exposure ( 50 , 51 ).…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus plantarum L15 Alleviates Colitis by Inhibiting LPS-Mediated NF-κB Activation and Ameliorates DSS-Induced Gut Microbiota Dysbiosis

Peng

Guo

et al. 2020

Front. Immunol.

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Previous studies have suggested that the Lactobacillus plantarum bacteria strain could be effective in ulcerative colitis (UC) management. However, its effects are strain-specific and the related mechanisms for its attenuating effects on UC remain unclear. This study aimed to elucidate the underlying mechanisms for the protective effect of L. plantarum on UC. Firstly, 15 L. plantarum strains were screened for potential probiotic characteristics with good tolerance to simulated human gastrointestinal transit and adhesion. Secondly, the inflammatory response of selected strains to the Caco-2 cells induced by lipopolysaccharide (LPS) was measured. Finally, an in vivo mouse model induced by dextran sulfate sodium (DSS) was used to assess the beneficial effects and likely action mechanisms the successfully screened in vitro strain, L. plantarum L15. In vitro results showed that L. plantarum L15 possessed the highest gastrointestinal transit tolerance, adhesion and reduction of pro-inflammatory abilities compared to the other screened strains. In vivo , high dose of L. plantarum L15 supplementation increased the body weight, colon length and anti-inflammatory cytokine production. Pro-inflammatory cytokine production, disease activity index (DAI) levels and myeloperoxidase (MPO) parameters decreased using this strain. In addition, L. plantarum L15 alleviated the histopathological changes in colon, modulated the gut microbiota, and decreased LPS secretion. The activities of this strain down-regulated the expression of TLR4 and MyD88 genes as well as genes associated with NF-κB signaling pathway. Our findings present L. plantarum L15 as a new probiotic, with promising application for UC management.

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Section: Discussionmentioning

confidence: 99%

Lactobacillus plantarum L15 Alleviates Colitis by Inhibiting LPS-Mediated NF-κB Activation and Ameliorates DSS-Induced Gut Microbiota Dysbiosis

Peng

Guo

et al. 2020

Front. Immunol.

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“…This treatment can reduce the expression of IL-8 and ICAM-1 mRNA and proteins in the colonic mucosa of patients with UC [ 46 ]. Previous studies have found that IL -12 and TNF- α expressions are significantly higher in UC rat colons, but herb-partitioned moxibustion reduces their expression [ 47 ]. Herb-partitioned moxibustion can also reduce the concentrations of IL-1 β , IL-6, and TNF- α from induced peripheral blood mononuclear cells (PBMCs) cultured supernatants of UC rats, relieving intestinal inflammation [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Herb-Partitioned Moxibustion Regulates the TLR2/NF-κB Signaling Pathway in a Rat Model of Ulcerative Colitis

Wang

Liu

Dong

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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The TLR2/NF-κB signaling pathway plays an important role in the pathomechanism of ulcerative colitis (UC); acupuncture and moxibustion can improve the damage in colonic tissues of UC, but the regulatory mechanism remains unknown. This study observed the effect of moxibustion on the TLR2/NF-κB signaling pathway at the Tianshu (ST25) and Qihai (CV6) acupuncture points in the UC rat. The result shows that TLR2, IRAK1, and IKK-b mRNA and protein levels in the colonic mucosa were significantly higher in the UC rats than in the control rats. Herb-partitioned moxibustion reduced the expression of TLR2, IRAK1, and IKK-b mRNA and proteins in the UC rats. Similarly, the expression of NF-κB was significantly increased and IFN-β and IL-10 were significantly decreased in the colonic mucosa of UC rats, but herb-partitioned moxibustion reduced the expression of IFN-β and upregulating the expression of IFN-β and IL-10 significantly. It indicates that herb-partitioned moxibustion can inhibit the expression of multiple signaling molecules of the TLR2 pathway effectively, and it may modulate the excessive local immune response by inhibiting TLR2 signaling, thereby promoting the repair of damaged colonic mucosa.

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“…For instance, many patients, especially in China and Asia areas, try to consult traditional medical doctors to manage their conditions by acupuncture and moxibustion [ 16 ]. Up till now, reports have demonstrated that acupuncture plus moxibustion or moxibustion alone can (1) manage the symptoms of abdominal pain, diarrhea, bloody stool, and consequent weight loss [ 17 ], (2) promote recovery of damaged intestinal tissues due to inflammation [ 18 ], and (3) suppress the inflammatory response of UC [ 19 ]. However, as to the Th17/Treg, so far very few studies of acupuncture and moxibustion have direct evidence to show that they can influence the balance of these two kinds of cells.…”

Section: Introductionmentioning

confidence: 99%

Regulating the Balance of Th17/Treg via Electroacupuncture and Moxibustion: An Ulcerative Colitis Mice Model Based Study

Sun

Zhang

Chengyulin

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Aim To investigate the relationship between the effects of electroacupuncture/moxibustion and the balance of Th17/Treg in treating ulcerative colitis (UC) and to preliminary compare the effects of the above two methods. Methods DSS-induced UC mice were treated by electroacupuncture and moxibustion. Disease activity index (DAI) was scored; intestinal pathological structure and ultrastructure were observed. The levels of IL-2, IL-6, IL-10, IL-17A, IL-17F, and TGF-β in plasma were measured by ELISA. The percentages of Treg and Th17 in spleen lymphocytes were analyzed by flow cytometry. Also, the expressions of TLR2, TLR4, RORγt, and FOXP3 in the distal colon were detected by immunohistochemistry or western blot. Results Both electroacupuncture and moxibustion can relieve UC. These effects are further supported by ELISA results. In addition, the ratio of Treg and Th17 in spleen lymphocytes and the expression of TLR2 and TLR4 are significantly improved. Also, the expression of RORγt and FOXP3 in distal colon were improved. Besides, the effect of moxibustion is better than that of electroacupuncture on TLR2, TLR4, and FOXP3 expression (P < 0.05). ConclusionBoth electroacupuncture and moxibustion may ameliorate UC by regulating the balance of Th17/Treg. Whether moxibustion has better efficacy than electroacupuncture needs further study.

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Moxibustion inhibits interleukin-12 and tumor necrosis factor alpha and modulates intestinal flora in rat with ulcerative colitis

Abstract: HPM treatment can regulate intestinal flora and inhibit the expression of TNF-α and IL-12 in the colon tissues of UC rats, indicating that HPM can improve colonic immune response.

Cited by 61 publications

References 40 publications

Lactobacillus plantarum L15 Alleviates Colitis by Inhibiting LPS-Mediated NF-κB Activation and Ameliorates DSS-Induced Gut Microbiota Dysbiosis

Lactobacillus plantarum L15 Alleviates Colitis by Inhibiting LPS-Mediated NF-κB Activation and Ameliorates DSS-Induced Gut Microbiota Dysbiosis

Herb-Partitioned Moxibustion Regulates the TLR2/NF-κB Signaling Pathway in a Rat Model of Ulcerative Colitis

Regulating the Balance of Th17/Treg via Electroacupuncture and Moxibustion: An Ulcerative Colitis Mice Model Based Study

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