Mp77-09 uropathogenic Escherichia Coli Motility Types: Genetics, Control, and Electron Microscopy

Abstract

Recurrent urinary tract infection (rUTI) is a common disease in women, but its pathogenesis is still unclear. The aim of this study is to investigate urothelial cytoskeleton and cell proliferation protein expression in patients with rUTI.METHODS: Female patients with rUTI (symptomatic UTI > 3 times in recent one year) were recruited. One month after recovery from rUTI (absence of white blood cell in urinary analysis), the patients were asked to provide bladder specimens by cold-cup endoscopic biopsy. Patients with stress urinary incontinence were asked to provide bladder specimen as control. The specimens were investigated with western blot and immunochemical staining for urothelial cytoskeleton and cell proliferation protein, including sonic hedgehog (SHH), tumor protein 63 (TP63), cytokeratin 5 (CK5), CK14, CK20, zonula occludens (ZO)-1, E-cadherin and tryptase. GAPDH was used as normalizing protein for the quantification.RESULTS: A total of 16 rUTI and 8 control bladder specimens were analyzed. The cell proliferation protein SHH and TP63 expression were significantly lower in the rUTI urothelium than that in control (Figure 1). The cytoskeleton protein expression in the rUTI urothelium was also significantly lower than that in control bladders, including CK5, CK14 and CK20. The immunochemical staining showed CK5 and TP63 expression were mainly located near the basal layer of urothelium, while the CK20 expression was main located in the umbrella cells (Figure 2). The immunochemical staining results also showed decreased immunoactivity of CK5, CK14, CK20, TP63 and SHH in the patients with rUTI (Figure 2). CONCLUSIONS: Our results showed deficits of urothelium cell proliferation and cytoskeleton protein in the patients who had clinically recovered from rUTI. This finding indicates the urothelium function is still unhealthy even though clinical bladder inflammation had been subsided.