MPS1 and MPS2: novel yeast genes defining distinct steps of spindle pole body duplication.

Winey, Mark; Goetsch, Loretta; Baum, Peter; Byers, Breck

doi:10.1083/jcb.114.4.745

Cited by 312 publications

(417 citation statements)

References 24 publications

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“…The resulting SPB is twice the size of those found in wild-type, and this SPB nucleates a monopolar spindle that contains about twice the normal number of MTs (Byers, 1981). Similarly, in the strains ndc-1 (Winey and Byers, 1992) and mps2-1 (Winey et al, 1991), an enlarged SPB is formed to accommodate the total number of spindle MTs in the resulting monopolar spindle. In the temperature-arrested cdc2O cells described here, both SPBs were enlarged and both nucleated an abnormally large number of MTs.…”

Section: Discussionmentioning

confidence: 99%

Three-dimensional analysis and ultrastructural design of mitotic spindles from the cdc20 mutant of Saccharomyces cerevisiae.

O’Toole

Mastronarde

Giddings

et al. 1997

MBoC

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The three-dimensional organization of mitotic microtubules in a mutant strain of Saccharomyces cerevisiae has been studied by computer-assisted serial reconstruction. At the nonpermissive temperature, cdc2O cells arrested with a spindle length of -2.5 ,um. These spindles contained a mean of 81 microtubules (range, 56-100) compared with 23 in wild-type spindles of comparable length. This increase in spindle microtubule number resulted in a total polymer length up to four times that of wild-type spindles. The spindle pole bodies in the cdc2O cells were -2.3 times the size of wild-type, thereby accommodating the abnormally large number of spindle microtubules. The cdc2O spindles contained a large number of interpolar microtubules organized in a "core bundle." A neighbor density analysis of this bundle at the spindle midzone showed a preferred spacing of -35 nm center-to-center between microtubules of opposite polarity. Although this is evidence of specific interaction between antiparallel microtubules, mutant spindles were less ordered than the spindle of wild-type cells. The number of noncore microtubules was significantly higher than that reported for wild-type, and these microtubules did not display a characteristic metaphase configuration. cdc2O spindles showed significantly more cross-bridges between spindle microtubules than were seen in the wild type. The cross-bridge density was highest between antiparallel microtubules. These data suggest that spindle microtubules are stabilized in cdc2O cells and that the CDC20 gene product may be involved in cell cycle processes that promote spindle microtubule disassembly.

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Section: Discussionmentioning

confidence: 99%

Three-dimensional analysis and ultrastructural design of mitotic spindles from the cdc20 mutant of Saccharomyces cerevisiae.

O’Toole

Mastronarde

Giddings

et al. 1997

MBoC

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“…It remains unclear whether the SAC component, Mps1p, also plays a role in centrosome duplication in higher eukaryotes [Fischer et al, 2004;Fisk et al, 2003;Liu et al, 2003;Stucke et al, 2004;Stucke et al, 2002] as it does in budding [Winey et al, 1991] but not in fission yeast [He et al, 1998]. The mammalian Ndc80p complex [McCleland et al, 2003], the ROD and ZW10 kinetochore proteins [Basto et al, 2000;Chan et al, 2000], the outer kinetochore protein Zwint-1 [Obuse et al, 2004;Wang et al, 2004a], and the kinetochore-associated NEK2A protein [Lou et al, 2004] are also required for the spindle checkpoint, indicating that the kinetochore's role in SAC signaling is evolutionarily conserved, although no ROD, ZW10, or Zwint-1 homologues have been identified in yeast.…”

Section: Higher Eukaryotesmentioning

confidence: 99%

SAC‐ing mitotic errors: How the spindle assembly checkpoint (SAC) plays defense against chromosome mis‐segregation

Kadura

Sazer

2005

Cell Motil. Cytoskeleton

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“…The data available at present do not differentiate between these possibilities. These uncertainties are not unique to espI for mutations in top2 (Holm et al, 1985), ndcl (Thomas and Botstein, 1986), and mpsl (Winey et al, 1991) similarly cause defects in nuclear division without leading to arrest of the cell cycle. Perhaps all of these genes represent a subset of nuclear functions that are not subject to checkpoint control.…”

Section: Molecular Analysis Of the Esp1 Gene Revealsmentioning

confidence: 99%

Requirement for ESP1 in the nuclear division of Saccharomyces cerevisiae.

McGrew

Goetsch

Byers

et al. 1992

MBoC

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100

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Mutations in the ESP1 gene of Saccharomyces cerevisiae disrupt normal cell-cycle control and cause many cells in a mutant population to accumulate extra spindle pole bodies. To determine the stage at which the esp1 gene product becomes essential for normal cell-cycle progression, synchronous cultures of ESP1 mutant cells were exposed to the nonpermissive temperature for various periods of time. The mutant cells retained viability until the onset of mitosis, when their viability dropped markedly. Examination of these cells by fluorescence and electron microscopy showed the first detectable defect to be a structural failure in the spindle. Additionally, flow cytometric analysis of DNA content demonstrated that massive chromosome missegregation accompanied this failure of spindle function. Cytokinesis occurred despite the aberrant nuclear division, which often resulted in segregation of both spindle poles to the same cell. At later times, the missegregated spindle pole bodies entered a new cycle of duplication, thereby leading to the accumulation of extra spindle pole bodies within a single nucleus. The DNA sequence predicts a protein product similar to those of two other genes that are also required for nuclear division: the cut1 gene of Schizosaccharomyces pombe and the bimB gene of Aspergillus nidulans.

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MPS1 and MPS2: novel yeast genes defining distinct steps of spindle pole body duplication.

Cited by 312 publications

References 24 publications

Three-dimensional analysis and ultrastructural design of mitotic spindles from the cdc20 mutant of Saccharomyces cerevisiae.

Three-dimensional analysis and ultrastructural design of mitotic spindles from the cdc20 mutant of Saccharomyces cerevisiae.

SAC‐ing mitotic errors: How the spindle assembly checkpoint (SAC) plays defense against chromosome mis‐segregation

Requirement for ESP1 in the nuclear division of Saccharomyces cerevisiae.

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