MR-elastography reveals degradation of tissue integrity in multiple sclerosis

Wuerfel, Jens; Paul, Friedemann; Beierbach, Bernd; Hamhaber, Uwe; Klatt, Dieter; Papazoglou, Sebastian; Zipp, Frauke; Martus, Peter; Braun, Jürgen; Sack, Ingolf

doi:10.1016/j.neuroimage.2009.06.018

Cited by 272 publications

(253 citation statements)

References 29 publications

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“…[117][118][119] In these soft tissues, the macroscopic mechanical behavior (stiffness) has highly correlated changes in the tissue composition and structure identified by histology on a microscopic scale. Therefore, it is reasonable to expect that changes in the structure and composition of engineered cartilage during growth will alter the MRE-derived stiffness.…”

Section: Magnetic Resonance Elastographymentioning

confidence: 99%

Monitoring Cartilage Tissue Engineering Using Magnetic Resonance Spectroscopy, Imaging, and Elastography

Kotecha

Klatt

Magin

2013

Tissue Engineering Part B: Reviews

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A key technical challenge in cartilage tissue engineering is the development of a noninvasive method for monitoring the composition, structure, and function of the tissue at different growth stages. Due to its noninvasive, three-dimensional imaging capabilities and the breadth of available contrast mechanisms, magnetic resonance imaging (MRI) techniques can be expected to play a leading role in assessing engineered cartilage. In this review, we describe the new MR-based tools (spectroscopy, imaging, and elastography) that can provide quantitative biomarkers for cartilage tissue development both in vitro and in vivo. Magnetic resonance spectroscopy can identify the changing molecular structure and alternations in the conformation of major macromolecules (collagen and proteoglycans) using parameters such as chemical shift, relaxation rates, and magnetic spin couplings. MRI provides high-resolution images whose contrast reflects developing tissue microstructure and porosity through changes in local relaxation times and the apparent diffusion coefficient. Magnetic resonance elastography uses low-frequency mechanical vibrations in conjunction with MRI to measure soft tissue mechanical properties (shear modulus and viscosity). When combined, these three techniques provide a noninvasive, multiscale window for characterizing cartilage tissue growth at all stages of tissue development, from the initial cell seeding of scaffolds to the development of the extracellular matrix during construct incubation, and finally, to the postimplantation assessment of tissue integration in animals and patients.

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Section: Magnetic Resonance Elastographymentioning

confidence: 99%

Monitoring Cartilage Tissue Engineering Using Magnetic Resonance Spectroscopy, Imaging, and Elastography

Kotecha

Klatt

Magin

2013

Tissue Engineering Part B: Reviews

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“…[6][7][8][9][10][11][12][13][14][15][16][17][18][19] In MRE, the sample is excited mechanically, and the response is measured with magnetic resonance imaging. The primary goal of the technique is early detection of tissue lesions by determining the elasticity coefficients from the wavelengths of the stress waves.…”

Section: Introductionmentioning

confidence: 99%

“…The primary goal of the technique is early detection of tissue lesions by determining the elasticity coefficients from the wavelengths of the stress waves. After Ehman 6 proposed MRE system, MRE has been used to determine mechanical properties of various parts of body such as brain, 7,12 breast, 8,10 skeletal muscle, 9 liver, 11,17,18 heart, 11 lung, 13 and head and neck 15 as a research phase. Although MRE may be applied to detect mechanical properties of gels, which span a broad range of elasticity coefficients, the excitation source must contain a broad range of frequency components from about ten hertz to several tens of kilohertz.…”

Section: Introductionmentioning

confidence: 99%

High spatial and temporal resolution measurement of mechanical properties in hydrogels by non-contact laser excitation

et al. 2016

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Gels have received increased attention as potential materials for biological materials because they can exhibit similar mechanical properties. One obstacle for using gels is that their mechanical properties are significantly altered by defects, such as an inhomogeneous crosslink density distribution. If these defects could be detected and the values and spatial distributions of mechanical properties in the gel could be determined, it would be possible to apply gels for several fields. To achieve the high spatial and temporal resolution measurement of mechanical properties in hydrogels, in our method, a conventional contact excitation device is replaced with a non-contact excitation using laser ablation for the input and magnetic resonance elastography to measure stress waves is replaced with the Schlieren method with a high-speed camera. Magnetic resonance elastography is a local measurement technique, and consequently, requires a lot of time to characterize a sample, as well as does not have sufficient spatial resolution to obtain a broad range of elasticity coefficients of gels. We use laser ablation to apply non-contact impulse excitations to gels to generate stress waves inside them. We can determine mechanical properties of gels using the stress waves’ propagation velocity.

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“…For example, the ECM undergoes stiffness alterations as a result of injury, development, or disease. Dynamic substrates are therefore favored as tissue-mimicking substrate models in mechanobiology studies 22,24,25 . Numerous synthetic, natural, two-dimensional, three-dimensional, static, and dynamic biomaterials have been developed to mimic tissue stiffness 1, 3,6,16,23,26 .…”

Section: Introductionmentioning

confidence: 99%

Preparation of DNA-crosslinked Polyacrylamide Hydrogels

Previtera

Langrana

2014

JoVE

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Mechanobiology is an emerging scientific area that addresses the critical role of physical cues in directing cell morphology and function. For example, the effect of tissue elasticity on cell function is a major area of mechanobiology research because tissue stiffness modulates with disease, development, and injury. Static tissue-mimicking materials, or materials that cannot alter stiffness once cells are plated, are predominately used to investigate the effects of tissue stiffness on cell functions. While information gathered from static studies is valuable, these studies are not indicative of the dynamic nature of the cellular microenvironment in vivo. To better address the effects of dynamic stiffness on cell function, we developed a DNA-crosslinked polyacrylamide hydrogel system (DNA gels). Unlike other dynamic substrates, DNA gels have the ability to decrease or increase in stiffness after fabrication without stimuli. DNA gels consist of DNA crosslinks that are polymerized into a polyacrylamide backbone. Adding and removing crosslinks via delivery of single-stranded DNA allows temporal, spatial, and reversible control of gel elasticity. We have shown in previous reports that dynamic modulation of DNA gel elasticity influences fibroblast and neuron behavior. In this report and video, we provide a schematic that describes the DNA gel crosslinking mechanisms and step-by-step instructions on the preparation DNA gels. Video LinkThe video component of this article can be found at

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MR-elastography reveals degradation of tissue integrity in multiple sclerosis

Cited by 272 publications

References 29 publications

Monitoring Cartilage Tissue Engineering Using Magnetic Resonance Spectroscopy, Imaging, and Elastography

Monitoring Cartilage Tissue Engineering Using Magnetic Resonance Spectroscopy, Imaging, and Elastography

High spatial and temporal resolution measurement of mechanical properties in hydrogels by non-contact laser excitation

Preparation of DNA-crosslinked Polyacrylamide Hydrogels

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