MR molecular imaging of the Her‐2/<i>neu</i> receptor in breast cancer cells using targeted iron oxide nanoparticles

Artemov, Dmitri; Mori, Noriko; Okollie, Baasil; Bhujwalla, Zaver M.

doi:10.1002/mrm.10406

Cited by 346 publications

(230 citation statements)

References 24 publications

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“…8 Specifically, a biotinylated targeting antibody is first introduced intravenously and is allowed to localize to the specific disease site. Streptavidin-labeled SPIO is then introduced to label each antibody with multiple contrast agents.…”

Section: Active Targetingmentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

et al. 2006

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Abstract-The field of molecular imaging has recently seen rapid advances in the development of novel contrast agents and the implementation of insightful approaches to monitor biological processes non-invasively. In particular, superparamagnetic iron oxide nanoparticles (SPIO) have demonstrated their utility as an important tool for enhancing magnetic resonance contrast, allowing researchers to monitor not only anatomical changes, but physiological and molecular changes as well. Applications have ranged from detecting inflammatory diseases via the accumulation of non-targeted SPIO in infiltrating macrophages to the specific identification of cell surface markers expressed on tumors. In this article, we attempt to illustrate the broad utility of SPIO in molecular imaging, including some of the recent developments, such as the transformation of SPIO into an activatable probe termed the magnetic relaxation switch.

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Section: Active Targetingmentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

et al. 2006

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“…Moreover, compared with changes in signal intensity obtained in gadolinium-enhanced T1-weighted images (typically 10%-20%), SPIO-enhanced T2*-weighted images display higher signal intensity change (typically over 30%) and make rendering of 3D BBB-disruption volume easier. Previous studies have investigated the potential usefulness of SPIO-enhanced imaging for cellular imaging (18 -20), molecular imaging (21,22), and drug delivery to the central nervous system (23). Potential future application of SPIO-enhanced MR imaging of BBB disruption include the study of immune response to focused ultrasound by using SPIO-labeled macrophages and monitoring of targeted drug delivery in the brain.…”

Section: Discussionmentioning

confidence: 99%

Magnetic resonance imaging enhanced by superparamagnetic iron oxide particles: Usefulness for distinguishing between focused ultrasound‐induced blood–brain barrier disruption and brain hemorrhage

Liu

Hsu

Chu

et al. 2008

Magnetic Resonance Imaging

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Purpose:To investigate the usefulness of a fully flow-compensated heavy T2*-weighted imaging enhanced by superparamagnetic iron oxide (SPIO) particles for distinguishing between focused ultrasound-induced disruption of bloodbrain barrier (BBB) and brain hemorrhage. Materials and Methods:Focused ultrasound (frequency: 1.5 MHz) was used to induce disruption of the BBB in 39 rats. Two T2*-weighted images were obtained before and after SPIO administration. Preenhanced T2*-weighted images were used to detect hemorrhage. Detection of BBB disruption was performed on SPIO-enhanced images. Thirty-four rats were sacrificed after magnetic resonance (MR) scanning for histological confirmation of brain lesions. Theremaining five animals were followed up for 35 days.Prussian blue staining was performed on histological sections to detect SPIO particles in the brain. Results:After SPIO injection the areas of BBB disruption in rat brain were significantly enlarged. The area of mismatch between the T2*-weighted images indicated a safe region where BBB opening occurred without hemorrhagic complications. In the longitudinal study, removal of SPIO occurred at a faster rate in hemorrhagic areas, albeit being closer to that occurring in the liver. The presence of SPIO was confirmed by Prussian blue staining in brain parenchyma and capillary endothelial cells in areas of BBB disruption.Conclusion: T2*-weighted images-either with and without SPIO enhancement-may differentiate focused ultrasound-induced BBB disruption from brain hemorrhage.

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“…20 Para-magnetic nanoparticles conjugated with antibodies to HER-2/neu which are expressed on breast cancer cells have been used with MRI to detect breast cancer cells in vitro. 30 …”

Section: Para-magnetic Nanoparticlesmentioning

confidence: 99%