mrflbA, encoding a putative FlbA, is involved in aerial hyphal development and secondary metabolite production in Monascus ruber M-7

Yang, Yinfei; Li, Li; X, Li; Shao, Yanchun; Chen, Fusheng

doi:10.1016/j.funbio.2011.11.005

Cited by 36 publications

(18 citation statements)

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“…It is recognized that the signal transduction pathway G‐protein, and a global regulator of secondary metabolism (LaeA) are involved in fungal secondary metabolism (Bok and Keller ; Yu ). The regulation of the G‐protein heterotrimer, consisting of α, β, and γ subunits (named Mga1, Mgb1, and Mgg1, respectively), and MrflbA (the regulator of Mga1) on development and secondary metabolite production was investigated in M. ruber (Li and others , ; Yang and others ). In M. ruber , Mga1, Mgb1, and Mgg1 have been shown to promote both sexual and asexual development and vegetative growth, but they repress the production of citrinin and pigments (Li and others , ).…”

Section: Monascus Molecular Biology a New Breakthrough (From The Latmentioning

confidence: 99%

“…In M. ruber , Mga1, Mgb1, and Mgg1 have been shown to promote both sexual and asexual development and vegetative growth, but they repress the production of citrinin and pigments (Li and others , ). An MrflbA ‐deleted mutant resulted in lower production of pigment and citrinin (Yang and others ). The downregulation of MpLaeA by transgenic antisense d‐MpLaeA cDNA resulted in less production of monacolin K in M. pilosus (Zhang and Miyake ), whereas overexpressing the laeA gene in M. pilosus remarkably increased the production of monacolin K and pigments (Lee and others ).…”

Section: Monascus Molecular Biology a New Breakthrough (From The Latmentioning

confidence: 99%

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Edible Filamentous Fungi from the Species Monascus: Early Traditional Fermentations, Modern Molecular Biology, and Future Genomics

Chen

Zhou

et al. 2015

Comp Rev Food Sci Food Safe

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Monascus spp. are filamentous fungi famous for their fermented products, especially red mold rice (RMR), a traditional fermented food in East Asian areas with a very long edible history documented back to the Han dynasty (BC 202-AD 220) in China. Nowadays, RMR and its related products involve a very large industry from artisanal traditional fermentations to food companies to medicine manufacturers, which are distributed worldwide. Modern studies have shown that Monascus spp. are able to produce abundant beneficial secondary metabolites, such as monacolins (cholesterollowering agents), γ -amino butyric acid (an antihypertensive substance), dimerumic acid (an antioxidant), and pigments (food-grade colorants), and some strains can also secrete citrinin, a nephrotoxic metabolite. Monascus-related studies have received much attention because of their wide applications. However, to our knowledge, no systematic review on the progress of Monascus research has ever been published. In this review, the progress of research on Monascus is summarized into 3 stages: Monascus fermentation, Monascus molecular biology, and Monascus genomics. This review covers the past history, current status, and future direction of Monascus research, contributing to a comprehensive understanding of Monascus research progress.

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Section: Monascus Molecular Biology a New Breakthrough (From The Latmentioning

confidence: 99%

Section: Monascus Molecular Biology a New Breakthrough (From The Latmentioning

confidence: 99%

Edible Filamentous Fungi from the Species Monascus: Early Traditional Fermentations, Modern Molecular Biology, and Future Genomics

Chen

Zhou

et al. 2015

Comp Rev Food Sci Food Safe

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199

154

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“…In severe cases, this resulted in bulbous structures at hyphal tips. Corresponding with in the lower mycelial dry weight production observed, the RGS proteins FlbA in A. fumigatus and MrflbA in Monascus ruber had been reported in control of autolysis and cell death (Yu et al ., ; Yang et al ., ; Shin et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…Taken together, RGS involvement in different signalling cascades is expected through the enhanced GTP hydrolysis at the Gα-subunit Gpa1 and/or Gpa3, thus terminating G-protein signalling and allowing for normal hyphal growth, completion of clamp formation and subsequent fruiting body formation (Raudaskoski and Viitanen, 1982; for review see Wang et al, 2013). Another role for RGSs was proposed in secondary metabolism, e.g., with sterigmatocystin or pigment production in Aspergillus nidulans (Yu et al, 1996;Han et al, 2004), or citrinin in Monascus ruber (Yang et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

The regulator of G‐protein signalling Thn1 links pheromone response to volatile production in Schizophyllum commune

Wirth

Kunert

Ahrens

et al. 2018

Environmental Microbiology

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The regulator of G-protein signalling, Thn1, is involved in sexual development through pheromone signalling in the mushroom forming basidiomycete Schizophyllum commune affecting hyphal morphology and mating interactions. Thn1 plays a key role in coordinating sesquiterpene production, pheromone response and sexual development. The gene thn1 is transcriptionally regulated in response to mating with a role in clamp cell development and hydrophobin gene transcription. Further, it negatively regulates cAMP signalling and secondary metabolism. Disruption of thn1 affects dikaryotization by reducing clamp fusion and development with predominant non-fused pseudoclamps. Enhanced protein kinase A (PKA) activities in Δthn1 strains indicate that Thn1 regulates pheromone signalling by de-activating G-protein α subunits, which control cAMP-dependent PKA. The repressed formation of aerial hyphae could be linked to a reduced metabolic activity and to a transcriptional down-regulation of hyd6 and sc3 hydrophobin genes. Thn1 was also shown to be necessary for the biosynthesis of sesquiterpenes and an altered spectrum of sesquiterpenes in Δthn1 is linked to transcriptional up-regulation of biosynthesis genes. Proteome analysis indicated changes in cytoskeletal structure affecting actin localization, linking the major regulator Thn1 to growth and development of S. commune. The results support a role for Thn1 in G-protein signalling connecting development and secondary metabolism.

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“…For the determination of citrinin, after the filtration described above, 10 mL of the filtrate was concentrated by a rotary vacuum evaporator and then the pellet was dissolved in 1 mL of methanol, which was subjected to HPLC analysis following previously described method (Yang et al 2012 ). A citrinin standard compound (Sigma, USA) was used to confirm the HPLC analysis.…”

Section: Methodsmentioning

confidence: 99%

NAD+-dependent HDAC inhibitor stimulates Monascus pigment production but inhibit citrinin

Zhou

Mao

et al. 2017

AMB Expr

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Monascus species are edible fungi due to the production of food colorant and other beneficial compounds. Hence, it has been an attractive thesis to improve their productivities. Increasing numbers of investigations revealed that regulating the activities of histone deacetylases can significantly perturb secondary metabolites (SM) production at a global level. In this study, dihydrocoumarin (DHC, an inhibitor of the Sirtuin family of NAD+-dependent deacetylases) was used to treat Monascus ruber for evaluating its effects on organism growth and SM production. The results revealed that the variation trends of colonial sizes, biomass and mycotoxin were in a dose-dependent manner. Generally, they decreased with the increased DHC concentrations in the designed range. But the variation trend of pigment was different. Comparison of SM profile, three new peaks occurred to the mycelia extractions from DHC-treated strain corresponding to molecular weights 402, 416 and 444, respectively. These three compounds were identified as Monasfluol B, Monascus azaphilone C and acetyl-monasfluol B (a new Monascus chemical pigment structure). In short, DHC can stimulate M. ruber strain to produce more pigment-like polyketides but inhibition of mycotoxin (citrinin).Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-017-0467-1) contains supplementary material, which is available to authorized users.

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mrflbA, encoding a putative FlbA, is involved in aerial hyphal development and secondary metabolite production in Monascus ruber M-7

Cited by 36 publications

References 36 publications

Edible Filamentous Fungi from the Species Monascus: Early Traditional Fermentations, Modern Molecular Biology, and Future Genomics

Edible Filamentous Fungi from the Species Monascus: Early Traditional Fermentations, Modern Molecular Biology, and Future Genomics

The regulator of G‐protein signalling Thn1 links pheromone response to volatile production in Schizophyllum commune

NAD+-dependent HDAC inhibitor stimulates Monascus pigment production but inhibit citrinin

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