MRI‐Visible siRNA Nanomedicine Directing Neuronal Differentiation of Neural Stem Cells in Stroke

Lu, Liejing; Wang, Yong; Zhang, Fang; Chen, Meiwei; Lin, Bingling; Duan, Xiaohui; Cao, Minghui; Zheng, Chushan; Mao, Jiaji; Shuai, Xintao; Shen, Jun

doi:10.1002/adfm.201706769

Cited by 38 publications

(34 citation statements)

References 59 publications

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“…The high gene transfection efficiency of MFIONs without external magnetic force is beneficial for their potential clinical translation. Very recently, Lu et al reported a rationally well‐designed SPIONs‐based nanoscale polymersome to achieve highly efficient siRNA delivery into neural stem cells (NSCs) without the assistance of external magnetic field, which promoted the recovery of neural function in acute ischemic stroke . Herein, 1D nanostructured MFIONs with high aspect ratios are designed to facilitate the internalizations of their payload genes into MSCs.…”

Section: Discussionmentioning

confidence: 99%

Ferrimagnetic Nanochains‐Based Mesenchymal Stem Cell Engineering for Highly Efficient Post‐Stroke Recovery

Zhang

et al. 2019

Adv Funct Materials

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Unsatisfactory post-stroke recovery has long been a negative factor in the prognosis of ischemic stroke due to the lack of pharmacological treatments. Mesenchymal stem cells (MSCs)-based therapy has recently emerged as a promising strategy redefining stroke treatment; however, its effectiveness has been largely restricted by insufficient therapeutic gene expression and inadequate cell numbers in the ischemic cerebrum. Herein, a non-viral and magnetic field-independent gene transfection approach is reported, using magnetosome-like ferrimagnetic iron oxide nanochains (MFIONs), to genetically engineer MSCs for highly efficient post-stroke recovery. The 1D MFIONs show efficient cellular uptake by MSCs, which results in highly efficient genetic engineering of MSCs to overexpress brainderived neurotrophic factor for treating ischemic cerebrum. Moreover, the internalized MFIONs promote the homing of MSCs to the ischemic cerebrum by upregulating CXCR4. Consequently, a pronounced recovery from ischemic stroke is achieved using MFION-engineered MSCs in a mouse model.

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Section: Discussionmentioning

confidence: 99%

Ferrimagnetic Nanochains‐Based Mesenchymal Stem Cell Engineering for Highly Efficient Post‐Stroke Recovery

Zhang

et al. 2019

Adv Funct Materials

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“…Besides their oncological use, SPIONs could also facilitate other novel therapeutic approaches which involve MRI. They were, for example, successfully applied in the model of ischemic stroke [ 57 ]. Cerebral stroke remains one of the most frequent causes of mortality or severe disability in developed countries [ 58 ].…”

Section: Summary Of Clinical Applications Of Spionsmentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticles—Current and Prospective Medical Applications

et al. 2019

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The recent, fast development of nanotechnology is reflected in the medical sciences. Superparamagnetic Iron Oxide Nanoparticles (SPIONs) are an excellent example. Thanks to their superparamagnetic properties, SPIONs have found application in Magnetic Resonance Imaging (MRI) and magnetic hyperthermia. Unlike bulk iron, SPIONs do not have remnant magnetization in the absence of the external magnetic field; therefore, a precise remote control over their action is possible. This makes them also useful as a component of the advanced drug delivery systems. Due to their easy synthesis, biocompatibility, multifunctionality, and possibility of further surface modification with various chemical agents, SPIONs could support many fields of medicine. SPIONs have also some disadvantages, such as their high uptake by macrophages. Nevertheless, based on the ongoing studies, they seem to be very promising in oncological therapy (especially in the brain, breast, prostate, and pancreatic tumors). The main goal of our paper is, therefore, to present the basic properties of SPIONs, to discuss their current role in medicine, and to review their applications in order to inspire future developments of new, improved SPION systems.

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“…[ 23,24 ] We have also demonstrated highly efficient gene regulation by using polymeric carriers to mediate pDNA and siRNA delivery. [ 25,26 ] A directed differentiation of neural stem cells was achieved through gene delivery with polymeric vector. More excitingly, incorporating the magnetic resonance imaging (MRI) contrast agents such as superparamagnetic iron oxide nanocrystals (SPION) may endow the vectors with MRI visibility, which offers a great opportunity to track the in vivo fate of the transplanted stem cells.…”

Section: Introductionmentioning

confidence: 99%

“…More excitingly, incorporating the magnetic resonance imaging (MRI) contrast agents such as superparamagnetic iron oxide nanocrystals (SPION) may endow the vectors with MRI visibility, which offers a great opportunity to track the in vivo fate of the transplanted stem cells. [ 26,27 ] Among available medical imaging modalities, MRI possesses the advantages of high spatial resolution, allowing 3D visualization of anatomic structures. [ 28–30 ] Nevertheless, the imaging sensitivity of MRI is much lower than that of the optical imaging.…”

Section: Introductionmentioning

confidence: 99%

Bimodal Imaging‐Visible Nanomedicine Integrating CXCR4 and VEGFa Genes Directs Synergistic Reendothelialization of Endothelial Progenitor Cells

Dong

et al. 2020

Advanced Science

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A major challenge to treat vascular endothelial injury is the restoration of endothelium integrity in which endothelial progenitor cells (EPCs) plays a central role. Transplantation of EPCs as a promising therapeutic means is subject to two interrelated processes, homing and differentiation of EPCs in vivo, and thus a lack of either one may greatly affect the outcome of EPC-based therapy. Herein, a polymeric nanocarrier is applied for the codelivery of CXCR4 and VEGFa genes to simultaneously promote the migration and differentiation of EPCs. Moreover, MRI T 2 contrast agent SPION and NIR dye Cy7.5 are also loaded into the nanocarrier in order to track EPCs in vivo. Based on the synergistic effect of the two codelivered genes, an improved reendothelialization of EPCs is achieved in a rat carotid denuded model. The results show the potential of this bimodal imaging-visible nanomedicine to improve the performance of EPCs in repairing arterial injury, which may push forward the stem cell-based therapy of cardiovascular disease.

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MRI‐Visible siRNA Nanomedicine Directing Neuronal Differentiation of Neural Stem Cells in Stroke

Cited by 38 publications

References 59 publications

Ferrimagnetic Nanochains‐Based Mesenchymal Stem Cell Engineering for Highly Efficient Post‐Stroke Recovery

Ferrimagnetic Nanochains‐Based Mesenchymal Stem Cell Engineering for Highly Efficient Post‐Stroke Recovery

Superparamagnetic Iron Oxide Nanoparticles—Current and Prospective Medical Applications

Bimodal Imaging‐Visible Nanomedicine Integrating CXCR4 and VEGFa Genes Directs Synergistic Reendothelialization of Endothelial Progenitor Cells

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