MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of Malus ‘Radiant’ to Fade From Red to Green

Meng, Jianwen; Wei, Jun; Chi, Rufei; Qiao, Yu-Hang; Zhou, Jing; Wang, Yilin; Wang, Han; Li, Houhua

doi:10.3389/fpls.2022.822340

Cited by 12 publications

(15 citation statements)

References 42 publications

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“…For example, Tang et al reported that the anthocyanin content gradually decreases during red fading in herbaceous peony leaves [ 1 ]. Decreasing anthocyanin content led to fading red color in Malus ‘Radiant’ leaves [ 2 ]. Flavonols, another subgroup of flavonoids, can also modulate plant organ color as co-pigments of anthocyanins [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

The Paeonia qiui R2R3-MYB Transcription Factor PqMYBF1 Positively Regulates Flavonol Accumulation

Zhang

Duan

Wang

et al. 2023

Plants

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Tree peony is a “spring colored-leaf” plant which has red leaves in early spring, and the red color of the leaves usually fades in late spring. Flavonols are one subgroup of flavonoids, and they affect the plant organs’ color as co-pigments of anthocyanins. To investigate the color variation mechanism of leaves in tree peony, PqMYBF1, one flavonol biosynthesis-related MYB gene was isolated from Paeonia qiui and characterized. PqMYBF1 contained the SG7 and SG7-2 motifs which are unique in flavonol-specific MYB regulators. Subcellular localization and transactivation assay showed that PqMYBF1 localized to the nucleus and acted as a transcriptional activator. The ectopic expression of PqMYBF1 in transgenic tobacco caused an observable increase in flavonol level and the anthocyanin accumulation was decreased significantly, resulting in pale pink flowers. Dual-luciferase reporter assays showed that PqMYBF1 could activate the promoters of PqCHS, PqF3H, and PqFLS. These results suggested that PqMYBF1 could promote flavonol biosynthesis by activating PqCHS, PqF3H, and PqFLS expression, which leads metabolic flux from anthocyanin to flavonol pathway, resulting in more flavonol accumulation. These findings provide a new train of thought for the molecular mechanism of leaf color variation in tree peony in spring, which will be helpful for the molecular breeding of tree peony with colored foliage.

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Section: Introductionmentioning

confidence: 99%

The Paeonia qiui R2R3-MYB Transcription Factor PqMYBF1 Positively Regulates Flavonol Accumulation

Zhang

Duan

Wang

et al. 2023

Plants

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“…The bimolecular fluorescence complementation (BiFC) assays were performed as described in previous reports (Meng et al ., 2022). MsMYB10 and MsMYB62‐like were amplified and inserted into the pSPYNE‐35S vector, whereas MsbHLH3 was amplified and inserted into the pSPYCE‐35S vector (Walter et al ., 2004).…”

Section: Methodsmentioning

confidence: 99%

“…domestica ‘Fuji’ fruit or M . spectabilis leaves were described previously (Hu et al ., 2022; Meng et al ., 2022).…”

Section: Methodsmentioning

confidence: 99%

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The eTM–miR858–MYB62‐like module regulates anthocyanin biosynthesis under low‐nitrogen conditions in Malus spectabilis

et al. 2023

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The anthocyanin content increases in Malus spectabilis leaves under low-nitrogen conditions. Noncoding RNAs are indicated to play key regulatory roles in anthocyanin biosynthesis. However, the functional roles of noncoding RNAs in anthocyanin biosynthesis under lownitrogen conditions remain elusive.In this study, miR858 was screened as a key regulator of anthocyanin biosynthesis under low-nitrogen conditions through whole-transcriptome sequencing. Then, we used miR858 as an entry point to explore the regulatory network of lncRNA-miRNA-mRNA by dual-luciferase reporter assays and GUS histochemical staining assays, as well as to identify the mechanism of this regulatory network in anthocyanin biosynthesis by both transient and stable transformation experiments in Malus.MiR858 overexpression increased total anthocyanin content. MiR858 acted by negatively regulating its target gene, MsMYB62-like, under the low-nitrogen condition. MsMYB62-like inhibited the expression of MsF3 0 H, thereby negatively regulating anthocyanin biosynthesis. In addition, eTM858-1 and eTM858-2 were identified as endogenous target mimics of miR858 that bind to miR858 to prevent cleavage of MsMYB62-like and thereby negatively regulate anthocyanin biosynthesis.The results clarify the mechanism through which the eTM-miR858-MYB62-like module regulates anthocyanin biosynthesis in Malus under low-nitrogen conditions.

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“…The extraction of phenolic compounds was described previously [ 8 , 47 ]. The extraction supernatant was absorbed and filtered through a 0.45 µm filter, which was analyzed by HPLC coupled with a diode array detector (DAD) (Shimadzu LC-2030C Liquid Chromatograph, Shimadzu, Kyoto, Japan; Inertsil C-18 column, 5.0 µm particle size, 4.6 mm × 250 mm).…”

Section: Methodsmentioning

confidence: 99%

A TCP Transcription Factor in Malus halliana, MhTCP4, Positively Regulates Anthocyanins Biosynthesis

Meng

Yin

Wang

et al. 2022

IJMS

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Anthocyanins belong to a group of flavonoids, which are the most important flower pigments. Clarifying the potential anthocyanins biosynthesis molecular mechanisms could facilitate artificial manipulation of flower pigmentation in plants. In this paper, we screened a differentially expressed gene, MhTCP4, from the transcriptome data of Malus halliana petals at different development stages and explored its role in anthocyanins biosynthesis. The transcriptome data and qRT-PCR analysis showed that the expression level of MhTCP4 gradually decreased from the flower color fades. Tissue specific expression analysis showed MhTCP4 was expressed in the petal, leaf, and fruit of M. halliana, and was highly expressed in the scarlet petal. Overexpression of MhTCP4 promoted anthocyanins accumulation and increased pigments in infected parts of M. ‘Snowdrift’ and M. ‘Fuji’ fruit peels. In contrast, when endogenous MhTCP4 was silenced, the anthocyanins accumulation was inhibited and pigments decreased in the infected peels. The qRT-PCR analysis revealed that overexpression or silence of MhTCP4 caused expression changes of a series of structural genes included in anthocyanins biosynthesis pathway. The yeast two-hybrid assays indicated that MhTCP4 did not interact with MhMYB10. Furthermore, the yeast one-hybrid assays indicated that MhTCP4 did not directly bind to the promoter of MhMYB10, but that of the anthocyanins biosynthesis genes, MhCHI and MhF3′H. Dual luciferase assays further confirmed that MhTCP4 can strongly activate the promoters of MhCHI and MhF3′H in tobacco. Overall, the results suggest that MhTCP4 positively regulates anthocyanins biosynthesis by directly activated MhCHI and MhF3′H in M. halliana flowers.

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MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of Malus ‘Radiant’ to Fade From Red to Green

Cited by 12 publications

References 42 publications

The Paeonia qiui R2R3-MYB Transcription Factor PqMYBF1 Positively Regulates Flavonol Accumulation

The Paeonia qiui R2R3-MYB Transcription Factor PqMYBF1 Positively Regulates Flavonol Accumulation

The eTM–miR858–MYB62‐like module regulates anthocyanin biosynthesis under low‐nitrogen conditions in Malus spectabilis

A TCP Transcription Factor in Malus halliana, MhTCP4, Positively Regulates Anthocyanins Biosynthesis

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