mRNA Expression Analysis of E-Cadherin, VEGF, and MMPs in Gastric Cancer: a Pilot Study

Kumar, Puneet; Sebastian, Arun; Verma, Khushi; Dixit, Ruhi; Kumari, Soni; Singh, Juhi; Tiwary, Satyendra K.; Narayan, Gopeshwar

doi:10.1007/s13193-020-01096-5

Cited by 5 publications

(5 citation statements)

References 40 publications

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“…In other words, well-differentiated and moderately differentiated carcinomas expressed at a high level (27.1% and 17.1%, respectively), whereas poorly differentiated carcinomas expressed at a low level (7.1 percent). These authors also reported on the findings of Hamed et al, [23], and Kumar et al, [24] in a separate study. Inactivation of E-cadherin may result in tumor cell separation due to the loss of intercellular adhesion.…”

Section: Discussionmentioning

confidence: 57%

The Expression of E-cadherin & HER2 in Gastric Carcinoma

El-Mihy

Hasby

Noseir

et al. 2022

JAMMR

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Background and Aim: Cell adhesion molecules (often referred to as cadherins) are glycoproteins found in the cell membrane. They regulate biological processes such as cell migration, differentiation, proliferation, and death (apoptosis). Her-2 is a proto-oncogene that belongs to the EGFR protein family. In cellular processes such as cell growth, regulates cellular activities such as proliferation, differentiation, and survival. This study aimed to determine the expression of E-cadherin and HER2 in the available histologic subtypes and evaluate if there is a correlation between E-cadherin and HER2 immunohistochemical expression in gastric carcinoma. Subjects & Methods: A total of 50 cases of stomach cancer were included in this study, all of which were obtained retrospectively between January 2017 and January 2020 from the Pathology Department, Tanta University, Egypt and Tanta Cancer Center archives. The samples were obtained from gastroplastectomy specimens then stained using the immunostaining approach described as follows: Deparaffinization and rehydration followed by using 3-hydroxy-4-napthylbenzaldehyde (3-OH-4-NHB) as a starting material then a smorgasbord of antigens then exposing to primary antibodies then exposing to a secondary biotinylated antibody, after that identifying of enzymes using streptavidin-labeled enzymes then preparing a color working reagent and last complexity in the development of color. Results: E-cadherin was statistically significantly high in tumors exhibiting aggressive clinicopathologic characteristics specifically in males, all histopathologic variants (except for poorly differentiated tubular adenocarcinoma and signet ring carcinoma), N stages, M stages, higher in absent vascular invasion cases but lower in present cases and the same with perineural invasion cases. Tumor site had no significant impact on the levels of E-cadherin. HER2 showed statistically insignificant correlations with all factors. E-cadherin and HER2 expression showed statistically insignificant relationship. Conclusion: Elevated E-cadherin expression is associated with tumors that exhibit aggressive clinicopathologic characteristics. HER2 was expressed positively in low-grade variants, but no correlation was established with clinical characteristics. E-cadherin and HER2 expression have no discernible relationship.

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Section: Discussionmentioning

confidence: 57%

The Expression of E-cadherin & HER2 in Gastric Carcinoma

El-Mihy

Hasby

Noseir

et al. 2022

JAMMR

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“…34 However, no significant difference was found in the levels of VEGF expression between the gastric cancer patients and the normal group. 35 In another study, it was found that 66.7% of all NPC patients showed a significantly elevated level of VEGF expression compared with the controls. 36 It is noted that, in a study, 76% of all NPC patients presented a high level of VEGF expression with no significant difference compared with controls.…”

Section: Discussionmentioning

confidence: 93%

“…34 However, no significant difference was found in the levels of VEGF expression between the gastric cancer patients and the normal group. 35 …”

Section: Discussionmentioning

confidence: 99%

Study of Three Potential Diagnostic Biomarkers in Nasopharyngeal Carcinoma Samples from Guilan, North of Iran

Moghaddam

Roushandeh

Roudkenar

et al. 2022

Int Arch Otorhinolaryngol

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Introduction Finding biomarkers for highly lethal cancers is a priority. Objective The current study was designed to understand the clinical significance of vascular endothelial growth factor (VEGF), latent membrane protein 1 (LMP1), and tumor necrosis factor-α (TNF-α) expression as the biomarkers, and evaluate their correlation with each other, in nasopharyngeal carcinoma (NPC) in the province of Guilan, North of Iran. Methods Gene expression was evaluated in 25 formalin-fixed paraffin-embedded (FFPE) blocks from cases of confirmed NPC and 20 FFPE samples of non-NPC by quantifying messenger ribonucleic acid (mRNA) and protein levels, using real-time polymerase chain reaction (PCR) and immunohistochemistry (IHC) methods, respectively. Furthermore, the correlations among the protein levels of different genes, along with the patients' demographic characteristics were assessed. Results Our findings on mRNA and protein levels demonstrated that the expression of the LMP1 gene in the NPC group was significantly elevated compared with that of the non-NPC group. In addition, the protein levels in the NPC group indicated a positive and significant correlation between LMP1 and VEGF expression. It was noted that both protein and mRNA levels showed no significant differences in the expression of TNF-α and VEGF genes between the NPC and control groups. Furthermore, there was no significant relationship between the expression of these proteins and the demographic characteristics of NPC patients. Conclusion Overall, a significant increase in LMP1 expression was observed in NPC patients, which may serve as a diagnostic biomarker for NPC. Also, LMP1 might be involved in NPC progression by inducing VEGF gene expression.

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“…The activation of MMP9 has been implicated in the invasion and metastasis of GC[ 28 ]. Furthermore, MMP2 and MMP-9 are dramatically associated with cell invasion and metastasis in particular; this progression may occur through the digestion of the extracellular matrix in the basement membrane[ 29 ]. Therefore, the outcomes have proved that FBXL6 expression downregulation reduced MMP2 and MMP9 protein expression and inhibited GC invasion and metastasis ability.…”

Section: Discussionmentioning

confidence: 99%

F-box and leucine-rich repeat 6 promotes gastric cancer progression via the promotion of epithelial-mesenchymal transition

Meng¹,

Hu²,

Xu³

2023

World J Gastrointest Oncol

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BACKGROUND F-box and leucine-rich repeat 6 (FBXL6) have reportedly been associated with several cancer types. However, the role and mechanisms of FBXL6 in gastric cancer (GC) require further elucidation. AIM To investigate the effect of FBXL6 in GC tissues and cells and the underlying mechanisms. METHODS TCGA and GEO database analysis was performed to evaluate the expression of FBXL6 in GC tissues and adjacent normal tissues. Reverse transcription-quantitative polymerase chain reaction, immunofluorescence, and western blotting were used to detect the expression of FBXL6 in GC tissue and cell lines. Cell clone formation, 5-ethynyl-2’-deoxyuridine (EdU) assays, CCK-8, transwell migration assay, and wound healing assays were performed to evaluate the malignant biological behavior in GC cell lines after transfection with FBXL6-shRNA and the overexpression of FBXL6 plasmids. Furthermore, in vivo tumor assays were performed to prove whether FBXL6 promoted cell proliferation in vivo . RESULTS FBXL6 expression was upregulated more in tumor tissues than in adjacent normal tissues and positively associated with clinicopathological characteristics. The outcomes of CCK-8, clone formation, and Edu assays demonstrated that FBXL6 knockdown inhibited cell proliferation, whereas upregulation of FBXL6 promoted proliferation in GC cells. Additionally, the transwell migration assay revealed that FBXL6 knockdown suppressed migration and invasion, whereas the overexpression of FBXL6 showed the opposite results. Through the subcutaneous tumor implantation assay, it was evident that the knockdown of FBXL6 inhibited GC graft tumor growth in vivo . Western blotting showed that the effects of FBXL6 on the expression of the proteins associated with the epithelial-mesenchymal transition-associated proteins in GC cells. CONCLUSION Silencing of FBXL6 inactivated the EMT pathway to suppress GC malignancy in vitro . FBXL6 can potentially be used for the diagnosis and targeted therapy of patients with GC.

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mRNA Expression Analysis of E-Cadherin, VEGF, and MMPs in Gastric Cancer: a Pilot Study

Cited by 5 publications

References 40 publications

The Expression of E-cadherin & HER2 in Gastric Carcinoma

The Expression of E-cadherin & HER2 in Gastric Carcinoma

Study of Three Potential Diagnostic Biomarkers in Nasopharyngeal Carcinoma Samples from Guilan, North of Iran

F-box and leucine-rich repeat 6 promotes gastric cancer progression via the promotion of epithelial-mesenchymal transition

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