mRNA Expression and Role of PPARγ and PPARδ in Bovine Preimplantation Embryos Depending on the Quality and Developmental Stage

Suwik, Katarzyna; Sinderewicz, Emilia; Boruszewska, Dorota; Kowalczyk-Zięba, Ilona; Staszkiewicz-Chodor, Joanna; Łukaszuk, Krzysztof; Wocławek-Potocka, Izabela

doi:10.3390/ani10122358

Cited by 6 publications

(5 citation statements)

References 42 publications

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“…Moreover, we analyzed day-8 bovine embryo implantation potential via an invasion assay, and we found that the area of invasion was significantly higher in the 3% Exo group (Figure 4d, p < 0.05). Recently, a study linked proliferator-activated receptor delta (PPARδ) expression with embryo developmental competence, and we found higher expression of PPARδ in the 3% Exo group as compared with the control group (Figure 4c, p < 0.05) [21,30]. Furthermore, the considerable difference in blastocyst hatching rates between the two groups corroborates our previous findings.…”

Section: Boec-exo Addition Affects Lipid Level In Mii-stage Bovine Oo...supporting

confidence: 89%

“…Recently, a study linked proliferator-activated receptor delta (PPARδ) expression with embryo developmental competence, and we found higher expression of PPARδ in the 3% Exo group as compared with the control group ( Figure 4 c, p < 0.05) [ 21 , 30 ]. Furthermore, the considerable difference in blastocyst hatching rates between the two groups corroborates our previous findings.…”

Section: Resultsmentioning

confidence: 99%

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BOEC–Exo Addition Promotes In Vitro Maturation of Bovine Oocyte and Enhances the Developmental Competence of Early Embryos

Wei

Idrees

Sidrat

et al. 2022

Animals

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Exosomes are nano-sized vesicles with abundant nucleic acids, proteins, lipids, and other regulatory molecules. The aim of this study was to examine the effect of BOEC-Exo on bovine in vitro oocyte maturation and in vitro embryo development. We found that a 3% Exo supplementation to IVM media significantly enhanced the oocyte maturation and reduced the accumulation of ROS in MII-stage bovine oocytes. Oocyte maturation related genes (GDF9 and CPEB1) also confirmed that 3% Exo treatment to oocytes significantly (p < 0.05) enhanced the oocyte maturation. Next, we cultured bovine cumulus cells and assessed the effects of 3% Exo, which showed a reduced level of apoptotic proteins (caspase-3 and p-NF-κB protein expressions). Furthermore, we examined the gap junction (CX43 and CX37) and cumulus cells expansion related genes (HAS2, PTX3, and GREM1) in cumulus–oocyte complexes (COCs), and all those genes showed significantly (p < 0.05) higher expressions in 3% Exo-treated COCs as compared with the control group. Moreover, peroxisome proliferator-activated receptors (PPARs) and lipid metabolism-related genes (CPT1 and FABP3) were also analyzed in both the control and 3% Exo groups and the results showed significant (p < 0.05) enhancement in the lipid metabolism. Finally, the oocytes matured in the presence of 3% Exo showed a significantly higher rate of embryo development and better implantation potential. Finally, we concluded that Exo positively influenced bovine oocyte in vitro maturation and improved the early embryo’s developmental competence.

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Section: Boec-exo Addition Affects Lipid Level In Mii-stage Bovine Oo...supporting

confidence: 89%

Section: Resultsmentioning

confidence: 99%

BOEC–Exo Addition Promotes In Vitro Maturation of Bovine Oocyte and Enhances the Developmental Competence of Early Embryos

Wei

Idrees

Sidrat

et al. 2022

Animals

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“…In the rat model of gestational diabetes mellitus (GDM), PPARγ expression in fetal rats was signi cantly different by sex, with elevated levels in the liver of male fetuses but not in female fetuses, explaining why there is considerable sexual dimorphism in adult IVF phenotypes [28] . Studies have shown that PPARγ is associated with different stages of embryo development generated in vitro by selected embryo quality markers (SOX2, OCT4, PLAC8, IGF1R) [29] .…”

Section: Discussionmentioning

confidence: 99%

A genome-wide association study to identify PPARγinvolved metabolic abnormalities by oxidative damage in IVF offspring

2022

Preprint

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Based on our previous animal model of abnormal metabolism, we combined bioinformatics methods to explore the mechanism of early embryo oxidative stress increasing metabolic risk in offspring. Methods: To screen the differentially expressed genes (DEGs) between IVF and naturally conceived offspring by bioinformatics methods, and to identify the co-gene characteristics and biological function mechanisms of DEGs and glucose and lipid metabolism related. Then, we constructed oxidative stress models in vitro and in vivo and performed functional analysis of early embryo differential genes caused by oxidative stress to explore the molecular mechanism of abnormal metabolism risk in IVF offspring. Results: In IVF offspring, we found 15 differentially expressed genes associated with disorders of glucose and lipid metabolism, and their biological functions are primarily associated with lipid metabolism. Zygote oxidative stress is a key factor affecting offspring health, and in different oxidative stress models, the level of ROS was significantly increased. More importantly, the PPAR pathway was altered in both IVF offspring and the early embryo oxidative stress model. In addition, we found decreased expression and nuclear localization of PPARγ in oxidative stress on IVF-derived embryos. Conclusion: The results suggest that PPARγ is involved in the development of embryos under oxidative stress. Oxidative stress can lead to genome-wide reprogramming of embryos and affect The long-term health of offspring through PPARγ.

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“…PPARγ also is highly expressed in animal reproductive tissues, including periimplantation pig uterus tissue, pig uterus chorionic membrane, dog placenta and placenta cotyledon of ewes (Levytska et al, 2014). The gene also is expressed in bovine embryos cultured in vitro at all stages of early development, i.e., 2‐, 4‐ and 16‐cell stage embryos, morula and blastula (Suwik et al, 2020). In addition, ligand‐dependent PPARγ activation is implicated in the regulation of cellular energy, lipid metabolism, cell proliferation and cell differentiation (Hernandez‐Quiles et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

PPARγ regulates lipid metabolism and viability of sheep trophoblast cells

Hao,

Liu,

Chen

et al. 2023

Reprod Domestic Animals

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Peroxisome proliferator‐activated receptor γ (PPARγ) is highly expressed in trophoblast tissues in pregnancy during which the protein participates in diverse events, including embryo implantation and placental formation. However, little is known about the role of PPARγ in embryonic development. This study investigated the function of PPARγ in sheep trophoblast cells. The coding sequence of sheep PPARγ encoded 475 amino acids and included one synonymou mutation compared with the sheep reference sequence for PPARγ. The PPARγ protein was localized in the nucleus and cytoplasm of sheep trophoblasts. The relative expression of PPARγ was elevated in cells treated with rosiglitazone and reduced following administration of GW9662. Activation of PPARγ promoted cell proliferation and mobility, but inhibited apoptosis. In addition, stimulation of PPARγ promoted the expression of lipid metabolism‐related genes FABP4 and PLIN2. The expression of prostaglandin metabolism‐related genes PLA2G4A, PTGS2 and PTGES also was upregulated significantly in trophoblast cells when PPARγ was activated. In contrast, activation of PPARγ did not impact expression of the prostaglandin‐related genes PGFS and SLCO2A1. At the same time, activation of PPARγ activity increased the ratio of PGE2 to PGF2α. Furthermore, fluorescence labelling showed that the numbers of cell lipid droplets increased after stimulation of PPARγ activity, but decreased when PPARγ was inhibited. In conclusion, PPARγ is critical for the regulation of lipid metabolism and prostaglandin synthesis and secretion in sheep trophoblast cells and also has a potent effect on cell proliferation and viability.

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mRNA Expression and Role of PPARγ and PPARδ in Bovine Preimplantation Embryos Depending on the Quality and Developmental Stage

Cited by 6 publications

References 42 publications

BOEC–Exo Addition Promotes In Vitro Maturation of Bovine Oocyte and Enhances the Developmental Competence of Early Embryos

BOEC–Exo Addition Promotes In Vitro Maturation of Bovine Oocyte and Enhances the Developmental Competence of Early Embryos

A genome-wide association study to identify PPARγinvolved metabolic abnormalities by oxidative damage in IVF offspring

PPARγ regulates lipid metabolism and viability of sheep trophoblast cells

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