mRNA expression of a cadmium-responsive gene is a sensitive biomarker of cadmium exposure in the soil collembolan Folsomia candida

Nakamori, Taizo; Fujimori, A.; Kinoshita, Keiji; Ban-Nai, Tadaaki; Kubota, Yoshihisa; Yoshida, Satoshi

doi:10.1016/j.envpol.2009.11.022

Cited by 33 publications

(22 citation statements)

References 23 publications

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“…The conserved N-terminal domain (interval 4-73) contains the thioredoxin-fold domain, and a C-terminal domain (interval 83-184) consists of alpha helices. As a whole, the nucleotide sequence of GST cDNA mainly agrees with that published previously (Nakamori et al 2010). In addition, some bases which had not been defined could be completely specified.…”

Section: Predicted Amino Acid Sequence From the Pcr-amplified Productsupporting

confidence: 74%

“…Cycling conditions were kept constant for all assays. Primers for target gene (forward primer: CATCAACATTG CCGACAAAC, reverse primer: ACGACAGCCTTGAA CTTTGC) were based on sequences published in GenBank AB509261.1 (Nakamori et al 2010), and YWHAZ was used as reference gene (forward primer: TCGCCCTCAA CTTTTCCGTT, reverse primer: TGCTATCGCTTCATC GAATGCT) (de Boer et al 2009). The characterization of reaction products was done via melting curve analysis and gel electrophoresis to make sure that no formation of primerdimer exists.…”

Section: Qpcr and Data Analysismentioning

confidence: 99%

“…PCR amplification of the cDNA sample was performed using primer pair targeting the 657-bp open reading frame of transcribed region. Primers (forward primer: ATGTCGACC TACAAGTTGAC, reverse primer: TTATTCGGAGT AATCGATGTG) were designed using reference nucleotide of F. candida messenger RNA (mRNA) for putative GST (GenBank accession number AB509261.1) (Nakamori et al 2010). PCR was performed in a Mastercycler® gradient (Eppendorf, Hamburg, Germany) for 40 cycles exploiting HotMaster™ Taq DNA Polymerase (5 PRIME, Hamburg, Germany) using cycling parameters as suggested in the user manual.…”

Section: Enzyme Assay and Protein Determinationmentioning

confidence: 99%

“…GSTs are known to be implicated in the detoxification of xenobiotics in F. candida. One isozyme was sequenced and submitted to GenBank (AB509261.1) as completed complementary DNA (cDNA) sequence (Nakamori et al 2010). Since GSTs are, in general, often inducible by xenobiotics and other substances, it has been suggested that levels of GSTs might be a promising indicator of exposure of an organism to chemical pollution (Fitzpatrick and Sheehan 1993).…”

Section: Introductionmentioning

confidence: 99%

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Effects of imidacloprid on detoxifying enzyme glutathione S-transferase on Folsomia candida (Collembola)

Sillapawattana

Schäffer

2016

Environ Sci Pollut Res

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Chemical analyses of the environment can document contamination by various xenobiotics, but it is also important to understand the effect of pollutants on living organisms. Thus, in the present work, we investigated the effect of the pesticide imidacloprid on the detoxifying enzyme glutathione S-transferase (GST) from Folsomia candida (Collembola), a standard test organism for estimating the effects of pesticides and environmental pollutants on non-target soil arthropods. Test animals were treated with different concentrations of imidacloprid for 48 h. Changes in steady-state levels of GST messenger RNA (mRNA) and GST enzyme activity were investigated. Extracted proteins were separated according to their sizes by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the resolved protein bands were detected by silver staining. The size of the glutathione (GSH) pool in Collembola was also determined. A predicted protein sequence of putative GSTs was identified with animals from control group. A 3-fold up-regulation of GST steady-state mRNA levels was detected in the samples treated with 5.0 mg L imidacloprid compared to the control, while a 2.5- and 2.0- fold up-regulation was found in organisms treated with 2.5 and 7.5 mg L imidacloprid, respectively. GST activity increased with increasing imidacloprid amounts from an initial activity of 0.11 μmol min mg protein in the control group up to 0.25 μmol min mg protein in the sample treated with the 5.0 mg L of pesticide. By contrast, the total amount of GSH decreased with increasing imidacloprid concentration. The results suggest that the alteration of GST activity, steady-state level of GST mRNA, and GSH level may be involved in the response of F. candida to the exposure of imidacloprid and can be used as biomarkers to monitor the toxic effects of imidacloprid and other environmental pollutants on Collembola.

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Section: Predicted Amino Acid Sequence From the Pcr-amplified Productsupporting

confidence: 74%

Section: Qpcr and Data Analysismentioning

confidence: 99%

Section: Enzyme Assay and Protein Determinationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of imidacloprid on detoxifying enzyme glutathione S-transferase on Folsomia candida (Collembola)

Sillapawattana

Schäffer

2016

Environ Sci Pollut Res

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“…Recent studies showed that the chemical-responsive gene is involved in the initial stress response at low doses of exposure, even at levels at which toxic effects are not observed (Nakamori et al 2010). It has been suggested that gene expression profiling has been performed to better identify biomarkers of toxicity to predict potential hazardous chemicals (Brulle et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Multilevel ecotoxicity assessment of polycyclic musk in the earthworm Eisenia fetida using traditional and molecular endpoints

et al. 2011

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The ecotoxicity assessment of galaxolide (HHCB) and tonalide (AHTN) was investigated in the earthworm Eisenia fetida using traditional and novel molecular endpoints. The median lethal concentration (LC(50)) for 7-day and 14-day exposures was 573.2 and 436.3 μg g(-1) for AHTN, and 489.0 and 392.4 μg g(-1) for HHCB, respectively. There was no observed significant effect on the growth rate of E. fetida after a 28-day exposure except that at the highest concentration (100 μg g(-1)) of AHTN and HHCB, whereas a significant decrease of cocoon production was found in earthworms exposed to 50 and 100 μg g(-1). To assess molecular-level effect, the expression of encoding antioxidant enzymes and stress protein genes were investigated upon sublethal exposures using the quantitative real time PCR assay. The expression level of SOD, CAT and calreticulin genes was up-regulated significantly, while the level of annetocin (ANN) and Hsp70 gene expression was down-regulated in E. fetida. Importantly, the level of ANN expression had a significant positive correlation with the reproduction rate of earthworms. Furthermore, the lowest observed effect concentration (LOECs) of ANN expression level was 3 μg g(-1) for AHTN and 10 μg g(-1) for HHCB, suggesting that ANN gene expression can serve as a more sensitive indicator of exposure to AHTN and HHCB than traditional endpoints such as cocoon production. The transcriptional responses of these genes may provide early warning molecular biomarkers for identifying contaminant exposure, and the data obtained from this study will contribute to better understand the toxicological effect of AHTN and HHCB.

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Biotic Ligand Modeling for the Effect of Major Cations on the Uptake of Cadmium in Folsomia candida Exposed in a Sand‐Solution Medium

Ardestani,

van Gestel

2024

Enviro Toxic and Chemistry

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Biotic ligand modeling (BLM) approaches are already applied to predict the bioavailability and possible risk of metals in surface water, but need further development for soils. The present study investigated the effect of major cations (Ca2+, Mg2+, Na+, K+, and H+) on cadmium bioaccumulation in the springtail Folsomia candida. To avoid the complexity of real soils and enable control of elemental speciation in the exposure medium, the animals were exposed to different cadmium concentrations in an inert quartz sand‐solution medium. Accumulation of cadmium in the animals was measured after 7 days exposure at different cation concentrations. Among the cations, only Ca2+ significantly affected the uptake of cadmium in the springtails. Mg2+ also had higher effects compared with other selected cations. Using a BLM approach, the uptake of cadmium in the animals predicted by taking into account both Ca2+ and Mg2+ activities correlated well with the measured values (R2 = 0.68). The final estimated conditional binding constants for cadmium (log KCd‐BL), Ca (log KCa‐BL), and Mg (log KMg‐BL) of 1.06, 2.14, and 1.23 L/mol, respectively, were in agreement with previously reported values. The match between predicted and measured uptake data confirms the applicability and usefulness of the BLM for predicting the bioavailability of cadmium to springtails and opens the way for its application in soil. Environ Toxicol Chem 2024;00:1–7. © 2024 SETAC

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mRNA expression of a cadmium-responsive gene is a sensitive biomarker of cadmium exposure in the soil collembolan Folsomia candida

Cited by 33 publications

References 23 publications

Effects of imidacloprid on detoxifying enzyme glutathione S-transferase on Folsomia candida (Collembola)

Effects of imidacloprid on detoxifying enzyme glutathione S-transferase on Folsomia candida (Collembola)

Multilevel ecotoxicity assessment of polycyclic musk in the earthworm Eisenia fetida using traditional and molecular endpoints

Biotic Ligand Modeling for the Effect of Major Cations on the Uptake of Cadmium in Folsomia candida Exposed in a Sand‐Solution Medium

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