mRNA expression of canine ATP10C, a P4-type ATPase, is positively associated with body condition score

Roshwalb, Sara C.; Gorman, Sarah; Hurst, Sarah; Bartges, Joseph W.; Agarwal, Shradha; Sommardahl, Carla S.; Odoi, Agricola; Dhar, Madhu

doi:10.1016/j.tvjl.2010.09.008

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…The following procedures were performed as described elsewhere [ 5 , 10 , 11 ]. Cells were washed with ice cold 1X PBS, and total RNAs were isolated using RNeasy Mini RNA kit (Qiagen), according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Transient Silencing of a Type IV P-Type ATPase,Atp10c, Results in Decreased Glucose Uptake in C2C12 Myotubes

Hurst

Minkin

Biggerstaff

et al. 2012

Journal of Nutrition and Metabolism

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Atp10c is a strong candidate gene for diet-induced obesity and type 2 diabetes. To identify molecular and cellular targets of ATP10C, Atp10c expression was altered in vitro in C2C12 skeletal muscle myotubes by transient transfection with an Atp10c-specific siRNA. Glucose uptake assays revealed that insulin stimulation caused a significant 2.54-fold decrease in 2-deoxyglucose uptake in transfected cells coupled with a significant upregulation of native mitogen-activated protein kinases (MAPKs), p38, and p44/42. Additionally, glucose transporter-1 (GLUT1) was significantly upregulated; no changes in glucose transporter-4 (GLUT4) expression were observed. The involvement of MAPKs was confirmed using the specific inhibitor SB203580, which downregulated the expression of native and phosphorylated MAPK proteins in transfected cells without any changes in insulin-stimulated glucose uptake. Results indicate that Atp10c regulates glucose metabolism, at least in part via the MAPK pathway, and, thus, plays a significant role in the development of insulin resistance and type 2 diabetes.

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Section: Methodsmentioning

confidence: 99%

Transient Silencing of a Type IV P-Type ATPase,Atp10c, Results in Decreased Glucose Uptake in C2C12 Myotubes

Hurst

Minkin

Biggerstaff

et al. 2012

Journal of Nutrition and Metabolism

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“…ATP10A has been implicated in the regulation of insulin-stimulated glucose uptake by plasma membrane mobilization of GLUT4-containing vesicles [116,117,129]. Moreover, mRNA expression levels of a canine ATP10A orthologue in visceral adipose tissue were found to be five times higher in obese dogs in comparison with lean dogs, suggesting an involvement of ATP10A in response to diet-induced obesity [130]. It remains to be demonstrated if ATP10A is directly involved in insulin-stimulated mobilization of GLUT4-containing vesicles to the plasma membrane, or whether ATP10A exerts its effects on glucose metabolism via regulation of insulin receptor-induced signalling.…”

Section: The (Patho) Physiological Function Of Mammalian P4 Atpasesmentioning

confidence: 99%

P4 ATPases: Flippases in Health and Disease

Mark

Elferink

Paulusma

2013

IJMS

117

104

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P4 ATPases catalyze the translocation of phospholipids from the exoplasmic to the cytosolic leaflet of biological membranes, a process termed “lipid flipping”. Accumulating evidence obtained in lower eukaryotes points to an important role for P4 ATPases in vesicular protein trafficking. The human genome encodes fourteen P4 ATPases (fifteen in mouse) of which the cellular and physiological functions are slowly emerging. Thus far, deficiencies of at least two P4 ATPases, ATP8B1 and ATP8A2, are the cause of severe human disease. However, various mouse models and in vitro studies are contributing to our understanding of the cellular and physiological functions of P4-ATPases. This review summarizes current knowledge on the basic function of these phospholipid translocating proteins, their proposed action in intracellular vesicle transport and their physiological role.

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Mammalian P4-ATPases and ABC transporters and their role in phospholipid transport

Coleman

Quazi

Molday

2013

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

132

107

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Transport of phospholipids across cell membranes plays a key role in a wide variety of biological processes. These include membrane biosynthesis, generation and maintenance of membrane asymmetry, cell and organelle shape determination, phagocytosis, vesicle tranfficking, blood coagulation, lipid homeostasis, regulation of membrane protein function, apoptosis among others. P4-ATPases and ATP binding cassette (ABC) transporters are the two principal classes of membranes proteins that actively transport phospholipids across cellular membranes. P4-ATPases utilize the energy from ATP hydrolysis to flip aminophospholipids from the exocytoplasmic (extracellular/lumen) to the cytoplasmic leaflet of cell membranes generating membrane lipid asymmetry and lipid imbalance which can induce membrane curvature. Many ABC transporters play crucial roles in lipid homeostasis by actively transporting phospholipids from the cytoplasmic to the exocytoplasmic leaflet of cell membranes or exporting phospholipids to protein acceptors or micelles. Recent studies indicate that some ABC proteins can also transport phospholipids in the opposite direction. The importance of P4-ATPases and ABC transporters is evident from the findings that mutations in many of these transporters are responsible for severe human genetic diseases linked to defective phospholipid transport.

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mRNA expression of canine ATP10C, a P4-type ATPase, is positively associated with body condition score

Cited by 3 publications

References 10 publications

Transient Silencing of a Type IV P-Type ATPase,Atp10c, Results in Decreased Glucose Uptake in C2C12 Myotubes

Transient Silencing of a Type IV P-Type ATPase,Atp10c, Results in Decreased Glucose Uptake in C2C12 Myotubes

P4 ATPases: Flippases in Health and Disease

Mammalian P4-ATPases and ABC transporters and their role in phospholipid transport

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