mRNA, microRNA and lncRNA as novel bladder tumor markers

Wieczorek, Edyta; Reszka, Edyta

doi:10.1016/j.cca.2017.12.009

Cited by 69 publications

(50 citation statements)

References 123 publications

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“…Accumulating data had suggested that lncRNAs, defined as non-coding RNA longer than 200 nts in length, could participate in the biology processes of cancers and meanwhile, various oncogenes and suppressor genes had been identified to be involved in the oncogenesis of BC [28–30]. Hence, it was imperative to find appropriate BC biomarkers which played an important role in the development and progression of disease.…”

Section: Discussionmentioning

confidence: 99%

High LINC01605 expression predicts poor prognosis and promotes tumor progression via up-regulation of MMP9 in bladder cancer

et al. 2018

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The advent of high-throughput sequencing methods has facilitated identification of novel long non-coding RNAs (lncRNAs), which have been demonstrated to play an important role in multiple tumors. Moreover, with the assistance of bioinformatics analysis, LINC01605 has been found to be up-regulated in bladder cancer (BC) tissues compared with normal tissues. Hence, the present study was to explore its specific biological role and related mechanism in BC. The relative expression level of LINC01605 was measured in a cohort of BC tissues with matched normal tissues as well as human BC cell lines by quantitative real-time PCR (qRT-PCR). Survival analysis was performed to explore the relationship between LINC01605 expression and the prognosis of BC patients. The biological function of LINC01605 was studied in vitroand in vivo, by means of CCK-8 assay, colony formation assay, transwell assay, and tumor xenografts mice model. LINC01605 was found to be frequently highly expressed in both human BC cells and tissues. Survival analysis indicated that high LINC01605 expression was associated with higher histological grade and clinical stages. In addition, down-regulated LINC01605 in BC cells could significantly inhibit the abilities of proliferation, migration, and invasion in vitro and knockdown of LINC01605 in subcutaneous xenograft tumor model could impede tumorigenesis in vivo. Mechanistically, LINC01605 could activate epithelial–mesenchymal transition (EMT) signaling pathway and promote the expression of matrix metallopeptidase (MMP) 9 (MMP9). In summary, our results shed light on that LINC01605, as a new prognostic biomarker, could promote the proliferation, migration, and invasion of BC cells via activating EMT signaling pathway and up-regulating MMP9 expression.

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Section: Discussionmentioning

confidence: 99%

High LINC01605 expression predicts poor prognosis and promotes tumor progression via up-regulation of MMP9 in bladder cancer

et al. 2018

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“…7 They are often aberrantly expressed in various squamous cell carcinomas, such as esophageal cancer, 8 prostate cancer, 9 bladder cancer, 10 and head and neck squamous cell carcinoma, 11 and function as oncogenes or tumor suppressors. 7 They are often aberrantly expressed in various squamous cell carcinomas, such as esophageal cancer, 8 prostate cancer, 9 bladder cancer, 10 and head and neck squamous cell carcinoma, 11 and function as oncogenes or tumor suppressors.…”

Section: Introductionmentioning

confidence: 99%

Knockdown of LINC01116 inhibits cell migration and invasion in head and neck squamous cell carcinoma through epithelial‐mesenchymal transition pathway

Chen

Zhang

et al. 2019

J of Cellular Biochemistry

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Long noncoding RNAs (lncRNAs) are linked to tumor development and progression. The aim of this study was to determine the prognostic significance and biological role of LINC01116 in head and neck squamous cell carcinoma (HNSCC). We identified 21 aberrantly expressed lncRNAs specific to HNSCC that were common in two microarray datasets. LINC01116 was highly overexpressed in HNSCC tissues and was correlated to shorter overall survival and relapse‐free survival duration, as analyzed by the online Gene Expression Profiling Interactive Analysis platform. LINC01116 was also overexpressed in oral squamous cell carcinoma and nasopharyngeal carcinoma tissues, and LINC01116 silencing significantly inhibited the migration and invasion capacities of both cell lines by blocking the epithelial‐mesenchymal transition process. In addition, 125 coexpressing genes were identified by circlncRNAnet, and were mainly located on human autosomes and enriched in transforming growth factor‐β signaling pathway. These findings indicate that LINC01116 might be a potential therapeutic target for HNSCC.

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“…Recently, several reports have highlighted the role of long non-coding RNAs (lncRNAs) as urine-based biomarkers for diagnosis of bladder cancer [1,[10][11][12][13][14][15][16][17][18][19][20][21][22]. Long non-coding RNAs are transcripts longer than 200 nucleotides that are not translated into protein [23].…”

Section: Ivyspring International Publishermentioning

confidence: 99%

“…Encouraged by these successes, in this study, we attempted to discover and evaluate the prediction power of a panel of lncRNAs biomarkers to discriminate bladder cancer from urocystitis. We chose totally sixteen lncRNAs as our candidate biomarkers, as suggested by previous studies [1,[10][11][12][13][14][15][16][17][18][19][20][21][22]. We conducted a study of 140 NMIBC patients and 140 urocystitis patients.…”

Section: Ivyspring International Publishermentioning

confidence: 99%

A Panel of Urinary Long Non-coding RNAs Differentiate Bladder Cancer from Urocystitis

Yu¹,

Wang²,

Han³

et al. 2020

J. Cancer

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Liquid biopsy is becoming a promising method for non-invasive cancer detection. In several proof-of-concept studies, long non-coding RNAs (lncRNAs) were found to be potential biomarkers for bladder cancer detection. The objective of this study was to discover a panel of cell-free, urinary lncRNAs as liquid biopsy biomarkers to non-invasively differentiate bladder cancer from chronic urocystitis. To this end, we collected urine samples from both bladder cancer patients and urocystitis patients. These samples were divided into discovery group and validation group. In the discovery group, the expression levels of 16 cell-free urinary lncRNAs were measured by qPCR to discover candidate biomarkers. The diagnostic performance of the candidate lncRNA biomarkers was then evaluated, which led to a panel of lncRNA biomarkers for bladder cancer detection. The performance of this panel of biomarkers was further evaluated in the validation group to see if these lncRNA biomarkers could discriminate the bladder cancer patients from urocystitis patients. We found that all of the 16 lncRNAs evaluated in this study demonstrated significant difference (p<0.05) of expression between bladder cancer patients and urocystitis patients. Nine lncRNAs provided decent diagnostic performance with area under the receiver operating characteristic (ROC) curve (AUC) reaching 0.70 or higher. We then selected the top four lncRNAs, namely UCA1-201, HOTAIR, HYMA1 and MALAT1, to form a panel of urinary biomarkers. Using this panel, bladder cancer patients could be discriminated from urocystitis patients, with sensitivity and specificity reaching 95.7% and 94.3%, respectively. Finally, we confirmed the applicability of the four-lncRNA panel in an independent validation study that included 60 bladder cancer patients and 60 urocystitis patients. Our study paves the way for further studies aimed at large-scale clinical tests of developing lncRNA biomarkers in urine for bladder cancer diagnostics.

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mRNA, microRNA and lncRNA as novel bladder tumor markers

Cited by 69 publications

References 123 publications

High LINC01605 expression predicts poor prognosis and promotes tumor progression via up-regulation of MMP9 in bladder cancer

High LINC01605 expression predicts poor prognosis and promotes tumor progression via up-regulation of MMP9 in bladder cancer

Knockdown of LINC01116 inhibits cell migration and invasion in head and neck squamous cell carcinoma through epithelial‐mesenchymal transition pathway

A Panel of Urinary Long Non-coding RNAs Differentiate Bladder Cancer from Urocystitis

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