mRNA profiling for body fluid identification

Haas, Cordula; Klesser, B.; Krätzer, A.; Bär, W.

doi:10.1016/j.fsigss.2007.10.064

Cited by 12 publications

(2 citation statements)

References 3 publications

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“…Recently, there has been considerable research into biomolecular markers for tissue identification. These include mRNA transcripts [ 1 ], micro-RNAs [ 2 , 3 ], proteomics [ 4 ], and DNA methylation patterns [ 5 ]. Although promising, the specificity of many of these systems is still being investigated and interpretation can require complex bioinformatic workflows.…”

Section: Introductionmentioning

confidence: 99%

Rapid differentiation of epithelial cell types in aged biological samples using autofluorescence and morphological signatures

et al. 2018

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Establishing the tissue source of epithelial cells within a biological sample is an important capability for forensic laboratories. In this study we used Imaging Flow Cytometry (IFC) to analyze individual cells recovered from buccal, epidermal, and vaginal samples that had been dried between 24 hours and more than eight weeks. Measurements capturing the size, shape, and fluorescent properties of cells were collected in an automated manner and then used to build a multivariate statistical framework for differentiating cells based on tissue type. Results showed that epidermal cells could be distinguished from vaginal and buccal cells using a discriminant function analysis of IFC measurements with an average classification accuracy of ~94%. Ultimately, cellular measurements such as these, which can be obtained non-destructively, may provide probative information for many types of biological samples and complement results from standard genetic profiling techniques.

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Section: Introductionmentioning

confidence: 99%

Rapid differentiation of epithelial cell types in aged biological samples using autofluorescence and morphological signatures

et al. 2018

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“…Nowadays, there are two principal lines of active research in body-fluid identification. One is dedicated to the development of mRNA (messenger ribonucleic acid) markers [6][7][8][9][10][11][12] based on the different mRNA sequences in each body fluid. The other is based on the use of spectrometric analytical techniques.…”

Section: Introductionmentioning

confidence: 99%

Emerging spectrometric techniques for the forensic analysis of body fluids

Zapata

Ossa

García-Ruiz

2015

TrAC Trends in Analytical Chemistry

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Body fluids are evidence of great interest in forensics because they allow identification of individuals through the study of DNA. After reviewing the tests and the methods that are currently being used by forensic practitioners for the detection of body fluids (e.g. blood, semen, saliva, vaginal fluid, urine and sweat), and after showing their main drawbacks and limitations, this work focuses on the review of emerging spectrometric techniques applied for the forensic analysis of body fluids. These techniques include the use of ultraviolet-visible, infrared (IR), Raman, X-ray fluorescence and nuclear magnetic resonance spectroscopy and mass spectrometry for investigating blood, semen, saliva, urine, vaginal fluid or sweat. Although all these spectrometric techniques seem to have a high potential to differentiate body fluids prior to DNA extraction, IR and Raman spectroscopy have shown the most promising results for discriminating stains from body fluids.

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Comparative analysis of SNaPshot and massively parallel sequencing for body fluid–specific DNA methylation markers

Kim,

Park,

Lee

2024

Electrophoresis

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The identification of tissue‐specific differentially methylated regions has significantly contributed to the field of forensic genetics, particularly in body fluid identification crucial for linking evidence to crimes. Among the various approaches to analyzing DNA methylation, the SNaPshot assay has been popularly studied in numerous researches. However, there is a growing interest in exploring alternative methods such as the use of massively parallel sequencing (MPS), which can process a large number of samples simultaneously. This study compares SNaPshot and MPS multiplex assays using nine cytosine‐phosphate‐guanine markers for body fluid identification. As a result of analyzing 112 samples, including blood, saliva, vaginal fluid, menstrual blood, and semen, both methods demonstrated high sensitivity and specificity, indicating their reliability in forensic investigations. A total of 92.0% samples were correctly identified by both methods. Although both methods accurately identified all blood, saliva, and semen samples, some vaginal fluid samples showed unexpected methylation signals at nontarget loci in addition to the target loci. In the case of menstrual blood samples, due to their complexity, independent typing criteria were applied, and successful menstrual blood typing was possible, whereas a few samples showed profiles similar to vaginal fluid. The MPS method worked better in vaginal fluid samples, and the SNaPshot method performed better in menstrual blood samples. This study offers valuable insights into body fluid identification based on the characteristics of the SNaPshot and MPS methods, which may help in more efficient forensic applications.

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mRNA profiling for body fluid identification

Cited by 12 publications

References 3 publications

Rapid differentiation of epithelial cell types in aged biological samples using autofluorescence and morphological signatures

Rapid differentiation of epithelial cell types in aged biological samples using autofluorescence and morphological signatures

Emerging spectrometric techniques for the forensic analysis of body fluids

Comparative analysis of SNaPshot and massively parallel sequencing for body fluid–specific DNA methylation markers

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