2000
DOI: 10.1017/s1355838200991660
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mRNA surveillance in mammalian cells: The relationship between introns and translation termination

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Cited by 43 publications
(26 citation statements)
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“…However, attempts to coimmunoprecipitate T7-hUpf2p and HA-eIF4A or HA-eIF4AIII have failed to detect an interaction (data not shown). Recent studies of S. cerevisiae have demonstrated that Upf1p interacts with Hrp1p, which has been proposed to mark transcripts at so-called downstream sequence elements (15) much as splicing-dependent proteins have been proposed to mark the exon-exon junctions of mammalian mRNAs (7,8,26,41,43,44,48,49). Therefore, another issue to be resolved is the relationship between those hUpf proteins that shuttle between nuclei and cytoplasm and the splicing-dependent mark, at least some component(s) of which must also shuttle, considering its role in not only nucleus-associated NMD but cytoplasmic NMD (41).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…However, attempts to coimmunoprecipitate T7-hUpf2p and HA-eIF4A or HA-eIF4AIII have failed to detect an interaction (data not shown). Recent studies of S. cerevisiae have demonstrated that Upf1p interacts with Hrp1p, which has been proposed to mark transcripts at so-called downstream sequence elements (15) much as splicing-dependent proteins have been proposed to mark the exon-exon junctions of mammalian mRNAs (7,8,26,41,43,44,48,49). Therefore, another issue to be resolved is the relationship between those hUpf proteins that shuttle between nuclei and cytoplasm and the splicing-dependent mark, at least some component(s) of which must also shuttle, considering its role in not only nucleus-associated NMD but cytoplasmic NMD (41).…”
Section: Discussionmentioning
confidence: 99%
“…This pathway surveys all translated mRNAs, whether they be normal or defective, in order to degrade those that prematurely terminate translation more than 50 to 55 nucleotides (nt) upstream of the final exon-exon junction (7,8,41,43,44,48,49)-a feature of most PTCs but not most normal termination codons (34). These and other data indicate that NMD is mechanistically linked to nuclear pre-mRNA splicing.…”
mentioning
confidence: 99%
“…Some models and some experimental results indicate that mechanistic distinctions between premature and normal termination lead to preferential association of Upf1 with mRNPs undergoing premature termination (Amrani et al 2004(Amrani et al , 2006Bühler et al 2006;Johansson et al 2007;). Other models and other data suggest that Upf1 associates with all terminating ribosomes, and specificity for premature termination events is achieved by subsequent Upf1 interactions with factors that could only remain mRNA-associated if termination had occurred upstream of its normal site (Peltz et al 1993;Le Hir et al 2000;Sun and Maquat 2000). Additional studies imply that specificity for hUpf1 is imparted by mRNA 3 ′ -UTR length (Hogg and Goff 2010).…”
Section: Introductionmentioning
confidence: 99%
“…S1), upstream of the stop codon and nefm 3′-UTR to protect against nonsense-mediated decay (reviewed in Sun and Maquat, 2000). As determined by immunohistochemistical analysis of hindbrain and spinal cord, and by quantifying fluorescence in whole-embryo extracts on partially denaturing SDS 12% polyacrylamide gels (fluorescent SDS PAGE), addition of this intron boosted protein expression by approximately threefold over that of the intronless construct ( Fig.…”
Section: Resultsmentioning
confidence: 99%