MRS Studies of Muscle and Heart in Obesity and Diabetes

Prompers, Jeanine J.; Nicolay, Klaas

doi:10.1002/9780470034590.emrstm1462

Cited by 3 publications

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“…While general aspects of 1 H-MRS can be found either in textbooks or in other articles in this special issue on MRS recommendations, this article will emphasize the anatomical, morphological and biochemical properties of skeletal muscle and how these influence the acquisition, appearance and analysis of skeletal muscle 1 H-MR spectra, which differ considerably from the MR spectra of other organs. Rather than summarize a list of seminal papers that can be found in reviews that cover the field, [1][2][3][4][5][6] we will focus on methodological considerations. Specific recommendations for the measurement and assessment of intramyocellular lipids, acetylcarnitine and carnosine, three metabolites which are the focus of the majority of studies and publications in metabolic, physiologic and clinically oriented journals, are given.…”

Section: Introductionmentioning

confidence: 99%

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Proton magnetic resonance spectroscopy in skeletal muscle: Experts' consensus recommendations

et al. 2020

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H-MR spectroscopy of skeletal muscle provides insight into metabolism that is not available noninvasively by other methods. The recommendations given in this article are intended to guide those who have basic experience in general MRS to the special application of 1 H-MRS in skeletal muscle. The highly organized structure of skeletal muscle leads to effects that change spectral features far beyond simple peak heights, depending on the type and orientation of the muscle. Specific recommendations are given for the acquisition of three particular metabolites (intramyocellular lipids, carnosine and acetylcarnitine) and for preconditioning of experiments and instructions to study volunteers.

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Section: Introductionmentioning

confidence: 99%

“…5 IMCL droplets (li-lipids, IMCL) in an electron microscopic image from skeletal muscle. Spherical droplets are close to mitochondria (mi) indicating the metabolically active role of IMCL.…”

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confidence: 99%

Proton magnetic resonance spectroscopy in skeletal muscle: Experts' consensus recommendations

et al. 2020

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Proton Magnetic Resonance Spectroscopy in Skeletal Muscle

Boesch¹

2021

Encyclopedia of Biophysics

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Bioenergetic functions in subpopulations of heart mitochondria are preserved in a non-obese type 2 diabetes rat model (Goto-Kakizaki)

Lai

Kummitha

Loy³

et al. 2020

Sci Rep

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A distinct bioenergetic impairment of heart mitochondrial subpopulations in diabetic cardiomyopathy is associated with obesity; however, many type 2 diabetic (T2DM) patients with high-risk for cardiovascular disease are not obese. In the absence of obesity, it is unclear whether bioenergetic function in the subpopulations of mitochondria is affected in heart with T2DM. To address this issue, a rat model of non-obese T2DM was used to study heart mitochondrial energy metabolism, measuring bioenergetics and enzyme activities of the electron transport chain (etc). oxidative phosphorylation in the presence of substrates for ETC and ETC activities in both populations of heart mitochondria in T2DM rats were unchanged. Despite the preservation of mitochondrial function, aconitase activity in T2DM heart was reduced, suggesting oxidative stress in mitochondria. Our study indicate that metabolic function of heart mitochondria is unchanged in the face of oxidative stress and point to a critical role of obesity in T2DM cardiomyopathy.www.nature.com/scientificreports www.nature.com/scientificreports/ between diastolic dysfunction and cardiac triglyceride levels which are higher in healthy obese subjects and lean and obese diabetic patients than lean healthy subjects 11,12 .Cardiac abnormalities in obese and type 2 diabetic patients have been investigated with animal models of obesity and type 1 diabetes (T1DM) and T2DM 6 in which cardiac contractile efficiency and mitochondrial metabolism showed progressive declines 13,14 with an increased reactive oxygen species (ROS) production and lipid peroxidation. Although both model of type 1 and 2 are used to study diabetic cardiomyopathy, differences in bioenergetic function exist between them. In T1DM mice fed a regular chow diet, cardiac dysfunction was reported without any mitochondrial respiration defects, but in T2DM mice fed with high-fat diet, insulin resistance was accompanied by impairment of oxidative phosphorylation 5 . Nevertheless, these studies did not investigate the subpopulations of heart mitochondria (subsarcolemmal and interfibrillar), which have been reported to be differently affected by cardiomyopathy in hamster 15 and mice with T1DM 16 and T2DM 17 .Among the animal models 18 of diabetic cardiomyopathy, Goto-Kakizaki (GK) rats 6,19 have the unique feature of being insulin-resistant without obesity 20,21 . The GK model was reported to have a mild cardiomyopathy characterized by diastolic dysfunction 20 . Increased susceptibility to oxidative stress was observed in GK heart mitochondria 22 , but bioenergetic functions were not reported.A previous study in skeletal muscle of GK rats showed preserved bioenergetic function in both mitochondrial subpopulations 23 . In the current study, we evaluated bioenergetic function in heart mitochondrial subpopulations of the same non-obese diabetic GK rats at 18 and 28 weeks and found that metabolic function is preserved in both subpopulations of mitochondria despite induced mitochondrial stress. Scientific RepoRtS |(2020) 10:54...

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MRS Studies of Muscle and Heart in Obesity and Diabetes

Cited by 3 publications

References 184 publications

Proton magnetic resonance spectroscopy in skeletal muscle: Experts' consensus recommendations

Proton magnetic resonance spectroscopy in skeletal muscle: Experts' consensus recommendations

Proton Magnetic Resonance Spectroscopy in Skeletal Muscle

Bioenergetic functions in subpopulations of heart mitochondria are preserved in a non-obese type 2 diabetes rat model (Goto-Kakizaki)

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