MRSA outbreak at a transplantation unit

Romanelli, Roberta Maia Castro; Clemente, Wanessa T.; Lima, Stella Sala Soares; Rezende, Edna Maria; Martinho, Gláucia Helena; Paiva, L.F.R.; Neves, F.A.C.; Madeira, J.G.C.; Amâncio, G.C.S.; Lima, Agnaldo Soares; Faria, Luciene Carrara Paula; Coutinho, Rosane Luiza

doi:10.1016/s1413-8670(10)70011-8

Cited by 7 publications

(2 citation statements)

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“…One of the major reasons for the rapid accumulation of bacterial strains displaying multidrug resistance is inappropriate prescription and dosage of therapeutic antibiotics (Rios et al, 2016), resulting in over‐usage. Bacteria can develop antibiotic resistance via several mechanisms, including: (1) enhanced multidrug efflux pump activity on the membrane (Leski & Tomasz, 2005); (2) expression of enzymes that effectively inactivate antimicrobial drugs (Rouveix, 2007); (3) reduced antibiotic uptake; and (4) modification of antimicrobial drug or their targets (Rios et al, 2016; Romanelli et al, 2010). Despite greater awareness of appropriate antibiotic treatments for different bacterial infections, the percentage of bacterial strains developing antibiotic resistance is still gradually increasing each year.…”

Section: Introductionmentioning

confidence: 99%

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SalmonellaYqiC exerts its function through an oligomeric state

Huang

Chen

et al. 2023

Protein Science

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Protein oligomerization occurs frequently both in vitro and in vivo, with specific functionalities associated with different oligomeric states. The YqiC protein from Salmonella Typhimurium forms a homotrimer through its C‐terminal coiled‐coil domain, and the protein is closely linked to the colonization and invasion of the bacteria to the host cells. To elucidate the importance of the oligomeric state of YqiC in vivo and its relation with bacterial infection, we mutated crucial residues in YqiC's coiled‐coil region and confirmed the loss of trimer formation using chemical crosslinking and Size Exclusion Chromatography coupled with Multiple Angle Light Scattering (SEC‐MALS) techniques. The yqiC‐knockout strain complemented with mutant YqiC showed significantly reduced colonization and invasion of Salmonella to host cells, demonstrating the critical role of YqiC oligomerization in bacterial pathogenesis. Furthermore, we conducted a protein‐protein interaction study of YqiC using a pulled‐down assay coupled with mass spectrometry analysis to investigate the protein's role in bacterial virulence. The results reveal that YqiC interacts with subunits of Complex II of the electron transport chain (SdhA and SdhB) and the β‐subunit of F0F1‐ATP synthase. These interactions suggest that YqiC may modulate the energy production of Salmonella and subsequently affect the assembly of crucial virulence factors, such as flagella. Overall, our findings provide new insights into the molecular mechanisms of YqiC's role in S. Typhimurium pathogenesis and suggest potential therapeutic targets for bacterial infections.This article is protected by copyright. All rights reserved.

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Section: Introductionmentioning

confidence: 99%

“…(3) reduced antibiotic uptake; and (4) modification of antimicrobial drug or their targets (Rios et al, 2016;Romanelli et al, 2010). Despite greater awareness of appropriate antibiotic treatments for different bacterial infections, the percentage of bacterial strains developing antibiotic resistance is still gradually increasing each year.…”

mentioning

confidence: 99%

SalmonellaYqiC exerts its function through an oligomeric state

Huang

Chen

et al. 2023

Protein Science

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Alternatives to overcoming bacterial resistances: State-of-the-art

Rios¹,

Moutinho

Pinto³

et al. 2016

Microbiological Research

232

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Worldwide, bacterial resistance to chemical antibiotics has reached such a high level that endangers public health. Presently, the adoption of alternative strategies that promote the elimination of resistant microbial strains from the environment is of utmost importance. This review discusses and analyses several (potential) alternative strategies to current chemical antibiotics. Bacteriophage (or phage) therapy, although not new, makes use of strictly lytic phage particles as an alternative, or a complement, in the antimicrobial treatment of bacterial infections. It is being rediscovered as a safe method, because these biological entities devoid of any metabolic machinery do not possess any affinity whatsoever to eukaryotic cells. Lysin therapy is also recognized as an innovative antimicrobial therapeutic option, since the topical administration of preparations containing purified recombinant lysins with amounts in the order of nanograms, in infections caused by Gram-positive bacteria, demonstrated a high therapeutic potential by causing immediate lysis of the target bacterial cells. Additionally, this therapy exhibits the potential to act synergistically when combined with certain chemical antibiotics already available on the market. Another potential alternative antimicrobial therapy is based on the use of antimicrobial peptides (AMPs), amphiphilic polypeptides that cause disruption of the bacterial membrane and can be used in the treatment of bacterial, fungal and viral infections, in the prevention of biofilm formation, and as antitumoral agents. Interestingly, bacteriocins are a common strategy of bacterial defense against other bacterial agents, eliminating the potential opponents of the former and increasing the number of available nutrients in the environment for their own growth. They can be applied in the food industry as biopreservatives and as probiotics, and also in fighting multi-resistant bacterial strains. The use of antibacterial antibodies promises to be extremely safe and effective. Additionally, vaccination emerges as one of the most promising preventive strategies. All these will be tackled in detail in this review paper.

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