MS/MS applications in biological problems

Favretto, Donata; Traldi, Pietro

doi:10.1002/mas.1280120504

Cited by 16 publications

(7 citation statements)

References 307 publications

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“…' In a recent review, Traldi gives important views of tandem mass spectrometry for cyclo(depsi)peptides. 6 More recently, an exhaustive review from Eckart summarizes all the strategies developed for sequencing cy~lopeptides.~ In the case of cyclodepsipeptides, Lange et al proposed two methodologies to determine the primary structure of destruxins', 9, lo which are natural toxins from Metarhizium anisopliae. The first is direct sequence determination by means of sequential carboxylate anions observed in conventional negative-ion FAB mass spectra.'…”

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confidence: 99%

Gas‐phase reactivity of protonated cyclodepsipeptidic toxins: The study of a systematic fragmentation pathway under fast‐atom bombardment desorption

Loutelier

Hubert

Marcual

et al. 1995

Rapid Comm Mass Spectrometry

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A systematic pathway of fragmentation for several protonated cyclohexadepsipeptides is demonstrated by positive-ion fast-atom bombardment linked-scan mass spectrometry. It involves classical sequential acylium and immonium ions, but also, unusual fragment ions characteristic of this family of compounds.Since fast-atom bombardment (FAB) and liquid secondary ion mass spectrometry (LSIMS) ionization techniques have been introduced, structural analyses of linear and cyclic peptides have improved. 1 . 2 3 3 Thus, numerous examples of primary structure elucidation have been r e p~r t e d .~. ' In a recent review, Traldi gives important views of tandem mass spectrometry for cyclo(depsi)peptides.6 More recently, an exhaustive review from Eckart summarizes all the strategies developed for sequencing cy~lopeptides.~ In the case of cyclodepsipeptides, Lange et al. proposed two methodologies to determine the primary structure of destruxins', 9, lo which are natural toxins from Metarhizium anisopliae. The first is direct sequence determination by means of sequential carboxylate anions observed in conventional negative-ion FAB mass spectra.' The second approach used positive-ion FAB tandem mass spectrometry with low9 and high" energy collision-induced dissociation (CID). Under the latter conditions, a rapid and unambiguous sequence elucidation of destruxin-E (DTX-E) was performed by two-and three-stage tandem mass spectrometry (MS' and MS3), sequential product-ion spectra and by reaction-intermediate scanning (MS3). These strategies concerned the two most active destruxins, i.e. DTX-E and DTX-A, which possess varied biological activity and more particularly insecticidal value." Due to the high insecticidal potential, a general strategy for the synthesis of such compounds was successfully developed. 12. l3 Thus, various synthetic analogues were prepared.I4,In the present work, we attempt to show that their rapid identification becomes possible by means of characteristic and diagnostic transitions, even using a conventional (EB) double-focusing mass spectrometer.We have analysed a series of six analogues including natural and synthetic compounds (Fig. 1) by positiveion fast-atom bombardment linked-scan mass spectrometry. DTX-A, which was previously analysed on a tandem mass spectrometer: is now examined for comparison with DTX-B and the four synthetic analogues. EXPERIMENTAL ProductsDestruxins from Meturhizium anisopliae (DTX-A and DTX-B) were purified at the Institut de Chimie des Substances Naturelles, CNRS-France.I6 Synthetic analogues (Lac-6 DTX-E, Hpy-6 DTX-E, Hpp-6 DTX-E and Hpy(TMS)-6 DTX-E) were prepared at the URA-CNRS 0468-France, according to the previously published methodology.". 13, '' Glycerol (G) and mnitrobenzyl alcohol (NBA) were purchased from Prolabo (France). Xenon and helium were provided by Air Liquide (France). Mass spectrometryMass spectra were obtained on a conventional E B geometry mass spectrometer JEOL model AX-500 equipped with a DEC data system (JEOL-Europe SA, France). Ionization was realized by...

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confidence: 99%

Gas‐phase reactivity of protonated cyclodepsipeptidic toxins: The study of a systematic fragmentation pathway under fast‐atom bombardment desorption

Loutelier

Hubert

Marcual

et al. 1995

Rapid Comm Mass Spectrometry

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“…Recently, the technique has been adopted as a useful structural probe for large polyatomic ions such as high mass ions generated from proteins, oligosaccharides, etc. [8][9][10] The current understanding of CAD of polyatomic ions has been derived mostly from earlier investigations on CAD of diatomic ions which are summarized in several review articles. 1-3 It is widely accepted that CAD occurs in two successive steps, namely, the collisional excitation step and the dissociation step…”

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confidence: 99%

Scattering angular distributions in collisionally activated dissociation of some high mass ions: Analysis of mass-analyzed ion kinetic energy peak shapes

Lee

Kim

1995

The Journal of Chemical Physics

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Articles you may be interested inA selected ion flow tube study of the reactions of NO+ and O+ 2 ions with some organic molecules: The potential for trace gas analysis of air A method has been developed to determine scattering angular distribution in collisionally activated dissociation ͑CAD͒ of high mass ions by analyzing translational energy spectra recorded by mass-analyzed ion kinetic energy spectrometry. Scattering angular distributions have been obtained for CAD of C 6 F 5 I ϩ˙, Cr͑CH 3 COCHCOCH 3 ͒ 3 ϩ˙, and Cs 5 I 4 ϩ with He. Large energy losses observed in the translational energy spectra are attributed to the elastic energy transfer to the collision gas accompanying collisional deflection. Deflections of the parent ions get larger as the translational energy for relative motion in the center-of-mass coordinate system decreases. This has been explained by the reactive line-of-centers model. Either vibrational excitation via momentum transfer or vibronic excitation via nonadiabatic interaction is compatible with the experimental data. On the other hand, electronic excitation via the Franck-Condon process is not a feasible mechanism to explain the excitation process in CAD of high mass ions at keV translational energy.

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“…14,15 However, sector mass spectrometers still have many performance attributes which cannot be matched by these newer techniques. For example, recent developments have led to very compact double focusing designs 16 for use in GC/MS.…”

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The design and performance of a high resolution translational energy loss spectrometer

Brenton

Lock

1997

Rapid Commun. Mass Spectrom.

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An instrumental development is described that achieved high energy resolving power when studying collisions between fast ion beams, at keV energies, interacting with thermal molecular and atomic gaseous targets. This technique is referred to as translational energy spectroscopy (TES) and can reveal spectroscopic information on the states of the participating species, the dynamics of the collision, lifetimes of species formed, state-to-state collision cross-sections and populations of states. The instruments described are based on an unique symmetrical arrangement of cylindrical electrostatic analysers. These are placed in a double focusing ion-optical geometry leading to very small first and second order image aberrations, sufficient to obtain a primary energy resolving power of 10 5 . Two such instruments were developed and their relative performances are compared experimentally and through computer modelling; the second, and larger, instrument has an improved sensitivity by an order of magnitude and a three times increase in resolving power. This has enabled collision spectroscopy to achieve performance similar to electron energy-loss spectroscopy and photoelectron spectroscopy, with a resolution on the unscattered ion beam measured to be 0.01 eV, at 3 keV collision energy, on energy loss processes of ≥ 0.03 eV. These developments allow TES to observe, (i) the vibrational/electronic spectroscopy of keV ion/molecule collisions; (ii) spin-selective spectra; (iii) the highly excited states close to and beyond the ionization limit of the target species; and (iv) involatile targets using a high temperature cell. The validity of optical quantum selection rules, relying on the electric-dipole interaction governing photon-induced transitions, has been tested for TES, which relies on the more general ion/neutral interaction potential responsible for collisional excitation. Spin conservation was found to be strongly adhered to, whilst orbital angular momentum and total angular momentum were not. The specific selection rules for homonuclear diatomics g < -> g and u < -> u were found to be allowed in TES, in contradiction to the case of optical spectroscopy, whilst there was some preliminary evidence that the optical Σ + -Σ -symmetry-forbidden transition was also forbidden for the TES reactions studied. For H 2 + projectiles, excitation to triplet states is strongly favoured over excitation to singlet states.

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MS/MS applications in biological problems

Cited by 16 publications

References 307 publications

Gas‐phase reactivity of protonated cyclodepsipeptidic toxins: The study of a systematic fragmentation pathway under fast‐atom bombardment desorption

Gas‐phase reactivity of protonated cyclodepsipeptidic toxins: The study of a systematic fragmentation pathway under fast‐atom bombardment desorption

Scattering angular distributions in collisionally activated dissociation of some high mass ions: Analysis of mass-analyzed ion kinetic energy peak shapes

The design and performance of a high resolution translational energy loss spectrometer

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