MS1 is essential for male fertility by regulating the microsporocyte cell plate expansion in soybean

Fang, Xiaolong; Sun, Xiaomin; Yang, Xiangdong; Li, Qing; Lin, Chung-Hung; Xu, Jie; Gong, Weili; Wang, Yifan; Liu, Lu; Zhao, Limei; Liu, Baohui; Qin, Jun; Zhang, Mengchen; Zhang, Chunbao; Kong, Fanjiang; Li, Meina

doi:10.1007/s11427-021-1973-0

Cited by 24 publications

(28 citation statements)

References 53 publications

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“…Similarly, knockout of GmPRR37 by the CRISPR system suggested that it can repress flowering under LD (Wang et al, 2020c). Two CRISPR/Cas9 gene-editing mutants of Glyma.13G114200 exhibited male sterility phenotype, confirming that it was the casual gene for GmMS1 for male sterility (Fang et al, 2021b;Jiang et al, 2021;Nadeem et al, 2021). CRISPR/Cas9 was also be applied in yield and seed qualityrelated trait modifications, such as to alter plant architecture by editing GmLHYs (Cheng et al, 2019) or SPL9 (Bao et al, 2019;Cai et al, 2018;Cheng et al, 2019), to increase seed number per pod by editing Ln (Cai et al, 2021), to reduce beany flavour by knocking out LOXs (Wang et al, 2020b), to increase isoflavone content by editing GmF3H1, GmF3H2 and GmFNSII-1 simultaneously (Zhang et al, 2020f) and to alter the fatty acid profiling by editing FAD2-2 (Al .…”

Section: Genome Editingmentioning

confidence: 94%

Progress in soybean functional genomics over the past decade

Zhang

Liu

Wang

et al. 2021

Plant Biotechnology Journal

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106

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Summary Soybean is one of the most important oilseed and fodder crops. Benefiting from the efforts of soybean breeders and the development of breeding technology, large number of germplasm has been generated over the last 100 years. Nevertheless, soybean breeding needs to be accelerated to meet the needs of a growing world population, to promote sustainable agriculture and to address future environmental changes. The acceleration is highly reliant on the discoveries in gene functional studies. The release of the reference soybean genome in 2010 has significantly facilitated the advance in soybean functional genomics. Here, we review the research progress in soybean omics (genomics, transcriptomics, epigenomics and proteomics), germplasm development (germplasm resources and databases), gene discovery (genes that are responsible for important soybean traits including yield, flowering and maturity, seed quality, stress resistance, nodulation and domestication) and transformation technology during the past decade. At the end, we also briefly discuss current challenges and future directions.

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Section: Genome Editingmentioning

confidence: 94%

Progress in soybean functional genomics over the past decade

Zhang

Liu

Wang

et al. 2021

Plant Biotechnology Journal

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106

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“…Subsequently, the function of MS12 (Glyma.10G117000) was also con rmed by QTL mapping and functional complementation of soybean gene in Arabidopsis cdc20.2 (cell division cycle 20.2) mutant (Zhang 2019). In 2021, both MS1 (Glyma.13G114200) and MS6 (Glyma.13G066600) have been identi ed by ne mapping (Fang, et al 2021;Jiang, et al 2021;Nadeem, et al 2021;). MS6 encodes a Tapetal Development and Functional 1 (TDF1) protein, a R2R3 MYB transcription factor, and predominantly expressed in anther, where it regulated the formation of pollen grain ).…”

Section: Gms System In Soybeanmentioning

confidence: 99%

“…MS6 encodes a Tapetal Development and Functional 1 (TDF1) protein, a R2R3 MYB transcription factor, and predominantly expressed in anther, where it regulated the formation of pollen grain ). Three studies have reported the mapping work of MS1 successively (Fang, et al 2021;Jiang, et al 2021;Nadeem, et al 2021). MS1, encoding a NPK1-ACTIVATING KINESIN 2 (NACK2) protein, participates in regulating cell plate formation after cytokinesis by directly in uencing phragmoplast expansion (Fang, et al 2021).…”

Section: Gms System In Soybeanmentioning

confidence: 99%

“…In soybean, the nuclear male sterile mutants ms4, ms1, ms6 and ms3 have been cloned in the recent years (Thu, et al 2019;Fang, et al 2021;Jiang, et al 2021;Nadeem, et al 2021;Hou, et al 2022). To speed up the large-scale commercial cultivation of hybrid soybean, it is time to consider where to put the investments, should we continue to count on the three-line hybrid system and looking for the ideal maintainer and restorer lines, or we can rely on the BMS systems to realize the commercialization of hybrid soybean.…”

Section: Introductionmentioning

confidence: 99%

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Male sterility and hybrid breeding in soybean

Fang¹,

Sun²,

Li³

et al. 2023

Preprint

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Hybrid breeding can help us to meet the challenge of the growing world population with limited agricultural land. The demand for soybeans is expected to grow, however the hybrid soybean is still in the process of commercialization even though considerable progress has been made in soybean genome and genetic studies in recent years. Here, we summarize recent advances on male sterility-based breeding programs and the current status of hybrid soybean breeding. A number of male-sterile lines with cytoplasmic male sterility (CMS), genic-controlled photoperiod/ thermo-sensitive male sterility (GMS) and nuclear male-sterile mutants have been identified in soybean. More than 40 hybrid soybean varieties have been bred using the CMS three-line hybrid system and the cultivation of hybrid soybean still under way. The key to speed up hybrid soybean breeding is to increase the out-crossing rate in an economical way. This review outlines the problems with current hybrid soybean breeding systems and explores the current effort in making the hybrid soybean a commercial success.

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“…With the explosion of genomic resources and the rapid development of the third generation hybrid rice technology, more effort has been invested in identifying GMS genes for hybrid breeding in crops. Recently, a few GMS genes have been cloned, namely ms4 (Thu et al, 2019), ms1 (Fang et al, 2021; Jiang et al, 2021; Nadeem et al, 2021), and ms6 (Yu et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

MALE STERILITY 3 encodes a plant homeodomain‐finger protein for male fertility in soybean

Fan

et al. 2022

JIPB

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Male-sterile plants are used in hybrid breeding to improve yield in soybean (Glycine max (L.) Merr.). Developing the capability to alter fertility under different environmental conditions could broaden germplasm resources and simplify hybrid production. However, molecular mechanisms potentially underlying such a system in soybean were unclear. Here, using positional cloning, we identified a gene, MALE STERILITY 3 (MS3), which encodes a nuclear-localized protein containing a plant homeodomain (PHD)-finger domain. A spontaneous mutation in ms3 causing premature termination of MS3 translation and partial loss of the PHD-finger. Transgenetic analysis indicated that MS3 knockout resulted in nonfunctional pollen and no selfpollinated pods, and RNA-seq analysis revealed that MS3 affects the expression of genes associated with carbohydrate metabolism. Strikingly, the fertility of mutant ms3 can restore under longd conditions. The mutant could thus be used to create a new, more stable photoperiod-sensitive genic male sterility line for two-line hybrid seed production, with significant impact on hybrid breeding and production.

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MS1 is essential for male fertility by regulating the microsporocyte cell plate expansion in soybean

Cited by 24 publications

References 53 publications

Progress in soybean functional genomics over the past decade

Progress in soybean functional genomics over the past decade

Male sterility and hybrid breeding in soybean

MALE STERILITY 3 encodes a plant homeodomain‐finger protein for male fertility in soybean

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