MSCs-engineered biomimetic PMAA nanomedicines for multiple bioimaging-guided and photothermal-enhanced radiotherapy of NSCLC

Abstract

Background The recently developed biomimetic strategy is one of the mostly effective strategies for improving the theranostic efficacy of diverse nanomedicines, because nanoparticles coated with cell membranes can disguise as “self”, evade the surveillance of the immune system, and accumulate to the tumor sites actively. Results Herein, we utilized mesenchymal stem cell memabranes (MSCs) to coat polymethacrylic acid (PMAA) nanoparticles loaded with Fe(III) and cypate—an derivative of indocyanine green to fabricate Cyp-PMAA-Fe@MSCs, which featured high stability, desirable tumor-accumulation and intriguing photothermal conversion efficiency both in vitro and in vivo for the treatment of lung cancer. After intravenous administration of Cyp-PMAA-Fe@MSCs and Cyp-PMAA-Fe@RBCs (RBCs, red blood cell membranes) separately into tumor-bearing mice, the fluorescence signal in the MSCs group was 21% stronger than that in the RBCs group at the tumor sites in an in vivo fluorescence imaging system. Correspondingly, the T1-weighted magnetic resonance imaging (MRI) signal at the tumor site decreased 30% after intravenous injection of Cyp-PMAA-Fe@MSCs. Importantly, the constructed Cyp-PMAA-Fe@MSCs exhibited strong photothermal hyperthermia effect both in vitro and in vivo when exposed to 808 nm laser irradiation, thus it could be used for photothermal therapy. Furthermore, tumors on mice treated with phototermal therapy and radiotherapy shrank 32% more than those treated with only radiotherapy. Conclusions These results proved that Cyp-PMAA-Fe@MSCs could realize fluorescence/MRI bimodal imaging, while be used in phototermal-therapy-enhanced radiotherapy, providing desirable nanoplatforms for tumor diagnosis and precise treatment of non-small cell lung cancer.

Clinical Roles of Risk Model Based on Differentially Expressed Genes in Mesenchymal Stem Cells in Prognosis and Immunity of Non-small Cell Lung Cancer

“…Several studies have confirmed the importance of the biological functions of MSCs in the progression of NSCLC (Fregni et al, 2018;Gu et al, 2020;Liang et al, 2020;Liu et al, 2021;Yin et al, 2021). MSCs are known to cause transcriptional changes in lung cancer cells, leading to increased expression of MMP9.…”

“…TME dynamics are inseparable from the progression of NSCLC. MSCs are an important component of the TME ( Zhao et al, 2018 ; Zheng et al, 2020 ; Yin et al, 2021 ). MSC-conditioned medium can promote EMT, invasion, and migration of lung cancer cells, and can inhibit cell proliferation and apoptosis.…”

“…In the TME, MSCs participate in material transportation and intercellular information transmission by secreting exosomes, and regulate tumor cell viability ( Liu et al, 2021 ; Mohr et al, 2021 ; Wang W. et al, 2021 ; Muralikumar et al, 2021 ; Yin et al, 2021 ). Co-culturing MSCs with pancreatic cancer cells shows enhanced expression of metastatic cytokines CCL2 and IL6, which are produced by the stem cells.…”

Cell-derived membrane biomimetic nanocarriers for targeted therapy of pulmonary disease

“…In addition, Yin et al utilized MSCs membrane to coat polymethacrylic acid (PMAA), which was loaded with iron and cypate, to structure Cyp-PMAA-Fe@MSCs. This carrier not only had high stability and good tumor accumulation, but also had excellent photothermal conversion efficiency, which was used in photothermal therapy of lung cancer( Yin et al, 2021 ).…”

Cetuximab Combined With Sonodynamic Therapy Achieves Dual-Modal Image Monitoring for the Treatment of EGFR-Sensitive Non-Small-Cell Lung Cancer

“…As an essential element for the human body, iron plays a central role in the process of DNA replication and is also an auxiliary factor for heme ( 21 , 22 ). Moreover, the application of iron-containing contrast agents in magnetic resonance imaging (MRI) has gradually matured due to the advantages of their good imaging effects, low toxicity, and low price ( 23 , 24 ). On the other hand, a large number of studies have shown that IR780 can be effectively imaged after high-intensity infrared radiation to mediate PTT, PDT and SDT in tumor tissue ( 25 ).…”