MSTN , CKM , and DMRT3 Gene Variants in Different Lines of Quarter Horses

Pereira, Guilherme Luís; Matteis, Rafael de; Regitano, Luciana Correia de Almeida; Chardulo, Luis Artur Loyola; Curi, Rogério Abdallah

doi:10.1016/j.jevs.2015.09.001

Cited by 21 publications

(23 citation statements)

References 24 publications

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“…The concentration and purity were set to be 10 ng/µL and 1.7 (260/280 ratio), respectively. After DNA extraction, all animals were genotyped for the g.22999655C>A SNP by PCR-RFLP according to Pereira et al (2016).…”

Section: Animals and Pedigreementioning

confidence: 99%

“…Moreover, allele A has been associated with good quality of pace and gait, when compared to animals with genotype CC (Andersson et al, 2012;Jäderkvist et al, 2015). Recent studies (Promerová et al, 2014;Manso Filho et al, 2015;Patterson et al, 2015;Pereira et al, 2016;Fonseca et al, 2017) reported genotypic and allelic frequencies for g.22999655C>A SNP, and found that marcha picada animals have a higher frequency of the A allele compared with animals exhibiting marcha batida. However, this relationship remains uncertain, and the genotypic frequencies observed suggest that although DMRT3 is associated with gait, it would not be the exclusive causative variant for the different types of gait in Mangalarga Marchador horses.…”

Section: Introductionmentioning

confidence: 99%

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Research Article Allelic and genotypic frequencies of the <i>DMRT3</i> gene in the Brazilian horse breed Mangalarga Marchador and their association with types of gait

Bussiman¹,

Santos²,

Silva³

et al. 2019

Genet. Mol. Res.

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The DMRT3 gene has been described as the main gene influencing gait in horses; however, most studies have examined its effects by comparing information from trotted and gaited individuals. Within the Brazilian Mangalarga Marchador breed, animals can present two types of gaits (marcha batida and marcha picada), which differ in the movement form-diagonal (marcha batida) or lateral (marcha picada). We examined a possible association of the g.22999655C>A DMRT3 SNP with the type of gait, in this two-gaits breed. The relationship between individuals was calculated for the two phenotypic groups, as well as the inbreeding coefficient and its trend over generations. The allelic and genotypic frequencies were estimated based on genotype data from 310 horses (205 marcha ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 18 (1): gmr18217 F.O. Bussiman et al. 2 batida; 105 marcha picada).Two threshold models were used to determine the proportion of phenotypic variance explained by DMRT3 genotypes. The average coancestry for animals with marcha batida was 0.04 ± 0.00, whereas in marcha picada it was 0.05 ± 0.01 (higher than the population mean of 0.04 ± 0.00). The inbreeding coefficient was 1.71% and 3.48% for marcha batida and marcha picada animals, respectively. Effects of the DMRT3 gene ranged from 9.94 to 10.77% (as a proportion of phenotypic variance) when included as an uncorrelated random effect and as a covariable, respectively. The genotypic frequencies of the DMRT3 and the patterns of kinship and inbreeding could be a result of selection in this breed, resulting in statistical confusion between the DMRT3 effects and population structure.

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Section: Animals and Pedigreementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Research Article Allelic and genotypic frequencies of the <i>DMRT3</i> gene in the Brazilian horse breed Mangalarga Marchador and their association with types of gait

Bussiman¹,

Santos²,

Silva³

et al. 2019

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…But recently, Pereira et al . () have proven that this polymorphism is not associated with racing performance in Quarter Horses. In this study, a new SNP marker of the same gene, not previously described, has shown statistical significance in young horses for long‐distance races by RRP (33.4%, p = 0.010), therefore denoting the importance of the product of this gene for the precocious selection of Spanish Trotter horses.…”

Section: Discussionmentioning

confidence: 95%

“…The association between the CKM gene and racing ability has been supported by Gu et al (2010), with the identification of the SNP g.15884567A>G. For long-distance races, CC in the CKM gene promotes better results. But recently, Pereira et al (2015) have proven that this polymorphism is not associated with racing performance in Quarter Horses. In this study, a new SNP marker of the same gene, not previously described, has shown statistical significance in young horses for long-distance races by RRP (33.4%, p = 0.010), therefore denoting the importance of the product of this gene for the precocious selection of Spanish Trotter horses.…”

Section: Discussionmentioning

confidence: 99%

Quantitative analysis of short‐ and long‐distance racing performance in young and adult horses and association analysis with functional candidate genes in Spanish Trotter horses

Rama

Valera

Membrillo

et al. 2016

J Animal Breeding Genetics

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The association of five candidate genes with sporting performance in young and adult Spanish Trotter horses (STHs) was performed according to a previous selection based on quantitative analysis of the trait time per kilometre (TPK). A total of 334 516 records of TPK from 5958 STHs were used to estimate the estimated breeding values (EBVs) at different age groups (young and adults horses) throughout the range of distances (1600-2700 m) using a bicharacter random regression model. The heritability estimated by distance ranged from 0.16 to 0.40, with a different range for the two age groups. Considering the animals with the best and the worst deregressed EBV, 321 STHs were selected for SNP genotyping in MSTN, COX4I2, PDK4, DMRT3 and CKM genes. An association analysis based on ridge and logistic regression revealed that the young trotters with genotype GG in PDK4 (p < 0.05) and AA of DMRT3 (p < 0.001) SNPs show the best potential in short-distance races, while those carrying the genotype AA in DMRT3 (p < 0.001) and CC in CKM (p < 0.05) genes seem to be the best in long-distance races. Adult trotters with genotype AA in DMRT3 also display greater speed (p < 0.05) and endurance (p < 0.001).

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“…In Brazil the effective number of animals in the racing line is relatively small compared to other lines, however its has substantial economic importance. Despite economic and scientific interest in this line and its racing aptitude, few efforts have been made to better understand the genetic mechanisms underlying this trait (Meira et al, 2014a(Meira et al, , 2014bPereira et al, 2016bPereira et al, , 2016a.…”

Section: Introductionmentioning

confidence: 99%

Genomic regions associated with performance in racing line of Quarter Horses

et al. 2018

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Quarter Horses breed for rancing are able to run short distances faster than any other breed. Although in Brazil the effective number of animals in the racing line is relatively smaller compared to the other lines, its economic importance is substantial. Despite economic and scientific interest in this athletic trait, few efforts have been made to better understand the genetic and physiological mechanisms underlying this trait. The objective of this study was to perform genome-wide association studies (GWAS) in Quarter Horses of the racing line using equine single nucleotide polymorphism (SNP) genotyping chips for prospecting chromosome regions, genes and polymorphisms related to maximum speed index. Genotypic and phenotypic data from 305 horses genotyped with the 54 k or 65 k chip were used. Additional 187 not genotyped animal with SI record were added in the study, for a total of 492 animals and 620 horses in the pedigree used for the GWAS analysis GWAS was performed by the single-step genomic best linear unbiased prediction (ssGBLUP) method using two approaches: ssGWAS1 in which only SNP effects are recalculated at each iteration, and ssGWAS2 in which SNP effects are recalculated from updated genomic estimated breeding values (GEBVs) at each iteration. Twenty-one regions that explained more than 1% of the total genetic variance (gVar) in the maximum speed index were identified by ssGWAS1 and 12 by ssGWAS2. More than 40% of gVar was explained by these regions in ssGWAS1 and about 30% in ssGWAS2. Among chromosomes that explained more than 1% of genetic variance, five were common to both methods (equine autosomal chromosomes 3, 10, 15, 22, 25). The capacity to capture the largest number of small-effect quantitative trait loci was greater for ssGWAS1 than ssGWAS2. A total of 108 genes were identified with the first approach and 59 with the second approach. The present results show that the racing performance of Quarter Horses, given by the maximum speed index, is a quantitative trait and that no major genes exist. In general, the genes identified by enrichment analysis are related to the neuromotor system, bone and tendon development, hormonal responses, muscle contraction, and energy and sterol metabolism. These functions are considered important for the trait studied, corroborating these genes as candidates for racing performance in the Quarter Horse breed.

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MSTN , CKM , and DMRT3 Gene Variants in Different Lines of Quarter Horses

Cited by 21 publications

References 24 publications

Research Article Allelic and genotypic frequencies of the <i>DMRT3</i> gene in the Brazilian horse breed Mangalarga Marchador and their association with types of gait

Research Article Allelic and genotypic frequencies of the <i>DMRT3</i> gene in the Brazilian horse breed Mangalarga Marchador and their association with types of gait

Quantitative analysis of short‐ and long‐distance racing performance in young and adult horses and association analysis with functional candidate genes in Spanish Trotter horses

Genomic regions associated with performance in racing line of Quarter Horses

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