2005
DOI: 10.1128/jb.187.17.6069-6074.2005
|View full text |Cite
|
Sign up to set email alerts
|

MtdC, a Novel Class of Methylene Tetrahydromethanopterin Dehydrogenases

Abstract: Novel methylene tetrahydromethanopterin (H 4 MPT) dehydrogenase enzymes, named MtdC, were purified after expressing in Escherichia coli genes from, respectively, Gemmata sp. strain Wa1-1 and environmental DNA originating from unidentified microbial species. The MtdC enzymes were shown to possess high affinities for methylene-H 4 MPT and NADP but low affinities for methylene tetrahydrofolate or NAD. The substrate range and the kinetic properties revealed by MtdC enzymes distinguish them from the previously char… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

0
16
0

Year Published

2005
2005
2016
2016

Publication Types

Select...
4
3
1

Relationship

1
7

Authors

Journals

citations
Cited by 16 publications
(16 citation statements)
references
References 30 publications
0
16
0
Order By: Relevance
“…Clones LWBAC10-4 and LWBAC10-10 were identified by screening a total of 1,000 clones of pool 10 of library 2 by hybridization with the fhcD probe consisting of a mixture of three divergent fhcD fragments that had been PCR amplified from this pool as described in Materials and Methods (FhcD3, FhcD7, and FhcD8). The same set of filters was hybridized with another probe, a divergent mtdA/B gene homolog identified previously (clone env97 [20]), and clone LWBAC10-4 was found to be positive for this probe.…”
Section: Resultsmentioning
confidence: 99%
See 2 more Smart Citations
“…Clones LWBAC10-4 and LWBAC10-10 were identified by screening a total of 1,000 clones of pool 10 of library 2 by hybridization with the fhcD probe consisting of a mixture of three divergent fhcD fragments that had been PCR amplified from this pool as described in Materials and Methods (FhcD3, FhcD7, and FhcD8). The same set of filters was hybridized with another probe, a divergent mtdA/B gene homolog identified previously (clone env97 [20]), and clone LWBAC10-4 was found to be positive for this probe.…”
Section: Resultsmentioning
confidence: 99%
“…A total of 14 potential ORFs were identified within the insert (Fig. 5), including three of the H 4 MPT-linked C 1 transfer gene homologs: the fae, the previously described mtdC (20), and an orf9 homolog. The predicted functions of the remaining 11 ORFs and their best hits in the databases are listed in Table S1 in the supplemental material.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Heterologously expressed Mtd from an ANME-1 archaeon catalyzed the same reaction as Mtd from methanogens, with similar catalytic specificity and cofactor dependence (214). In addition to F 420 -dependent enzymes, NAD(P)-dependent methylenetetrahydromethanopterin dehydrogenases have been characterized that have central roles in the formaldehyde assimilation pathways of aerobic methylotrophic bacteria (301,302). …”
Section: Adf: F 420 -Reducing Secondary Alcohol Dehydrogenasementioning
confidence: 99%
“…This is also the case within B. marina DSM 3645 T , whose fae1 gene has the third highest codon adaptation index (CAI) of all of its genes, but not for the P. maris DSM 8797 T fae gene. In all four genomes, fae1 is located upstream of a gene coding for the recently discovered methylene tetrahydromethanopterin gene mtdC (Vorholt et al, 2005). In R. baltica SH 1 T , B. marina DSM 3645…”
Section: Ecology Of Planctomycete D Woebken Et Almentioning
confidence: 99%