MtPIN1 and MtPIN3 Play Dual Roles in Regulation of Shade Avoidance Response under Different Environments in Medicago truncatula

Zhang, Xue; Liu, Lu; Wang, Hongfeng; Gu, Zhi-Qun; Liu, Yafei; Wang, Minmin; Wang, Min; Xu, Yiteng; Shi, Qingbiao; Li, Gang; Tong, Jianhua; Xiao, Langtao; Wang, Zeng‐Yu; Mysore, Kirankumar S.; Wen, Jiangqi; Zhou, Chuanen

doi:10.3390/ijms21228742

Cited by 4 publications

(4 citation statements)

References 61 publications

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“…Specifically, low R:FR‐induced auxin biosynthesis enhances PIN3's abundance and lateral localization, which promotes auxin flux to the epidermis. Moreover, loss of function of MtPIN1 and MtPIN3 disrupts shade‐avoidance responses and reduces petiole length in Medicago truncatula (Zhang et al, 2020). Our previous results indicated that clathrin modulates the subcellular localization of PIN3 and auxin maxima during hook formation as well as blue light (BL)‐triggered hypocotyl phototropism in Arabidopsis (Yu et al, 2016; Zhang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Clathrin light chains regulate hypocotyl elongation by affecting the polarization of the auxin transporter PIN3 in Arabidopsis

Yin

Sun

et al. 2021

JIPB

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PIN-FORMED (PIN)-dependent directional auxin transport is crucial for plant development. Although the redistribution of auxin mediated by the polarization of PIN3 plays key roles in modulating hypocotyl cell expansion, how PIN3 becomes repolarized to the proper sites within hypocotyl cells is poorly understood. We previously generated the clathrin light chain clc2-1 clc3-1 double mutant in Arabidopsis thaliana and found that it has an elongated hypocotyl phenotype compared to the wild

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Section: Introductionmentioning

confidence: 99%

Clathrin light chains regulate hypocotyl elongation by affecting the polarization of the auxin transporter PIN3 in Arabidopsis

Yin

Sun

et al. 2021

JIPB

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“…Nevertheless, PIN3 , which is a member of the PIN family, has rarely been reported to be involved in the establishment of leaf adaxial-abaxial polarity. The leaves of the mtpin1/mtpin3 double mutant displayed an abnormal abaxial surface only in Medicago truncatula ( Zhang et al., 2020 ). In our study, BpPIN3 suppressed expression transgenic lines (RE lines) showed leaf rolling; however, this phenotype was distinct from that of the mtpin1/mtpin3 double mutant in that the adaxial showed an upward rolling ( Figure 2C ).…”

Section: Discussionmentioning

confidence: 99%

Molecular mechanism of leaf adaxial upward curling caused by BpPIN3 suppression in Betula pendula

et al. 2022

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Leaves are one of the vegetative organs of plants that are essential for plant growth and development. PIN-FORMED (PINs) gene is an indoleacetic acid (IAA) transporter that plays a critical role in leaf development. To determine the function of BpPIN3 in leaf polarity formation in Betula pendula, the transgenic lines with BpPIN3 overexpression (OE) and BpPIN3-reduced expression (RE) were analyzed using the Agrobacterium-mediated method. The RE lines displayed the characteristics of leaf margin adaxial upward curling, with lower expression of BpPIN3 resulting in greater rolling. Tissue localization of IAA in the auxin GUS reporter system proved that auxin in the RE was mainly distributed in the secondary veins, palisade tissues, and epidermal cells in the leaf margin area. The auxin content in the leaf margin area was significantly greater than that in the main vein tissue. The cell density of the palisade tissue and the ratio of palisade tissue to spongy tissue in the curled leaf margin of the RE lines were found to be significantly decreased. RNA-seq analysis revealed that the RE hormone-signaling pathway genes were significantly enriched compared with those of the OE and WT lines; in particular, the auxin response-related genes SAURs (i.e., SAUR23, SAUR24, SAUR28, and SAUR50) and GH3.10 were found to be significantly upregulated. qRT-PCR analysis indicated that BpPIN3 expression at the leaf margin was significantly lower than that near the main vein in the RE lines. In contrast, the expression levels of SAURs and GH3.10 were significantly higher than those near the midrib. In conclusion, BpPIN3 regulates the expression of auxin response-related genes and the polar transport of auxin to change the polar form of the proximal and distal axes of birch leaves.

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“…The expression of most PINs was up-regulated compared with full light conditions, and those in group VI (PIN3/4/7) showed the same response trend ( Figure 9 a). PIN3/4/7 subgroups such as A. thaliana AtPIN3 and Medicago truncatula MtPIN3 were also found to be involved in plant shading responses [ 66 , 67 ], so it was further speculated that the M. micrantha PINs may promote the rapid elongation of the stem under low light by regulating the auxin level of the stem. Similarly, under defoliation stress, M. micrantha maintained stem growth by improving the photosynthesis rate and increasing the amount of auxin and other phytohormones [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Characterization of PIN Auxin Efflux Carrier Gene Family in Mikania micrantha

Chen

Cai

Chen

et al. 2022

IJMS

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Mikania micrantha, recognized as one of the world’s top 10 pernicious weeds, is a rapidly spreading tropical vine that has invaded the coastal areas of South China, causing serious economic losses and environmental damage. Rapid stem growth is an important feature of M. micrantha which may be related to its greater number of genes involved in auxin signaling and transport pathways and its ability to synthesize more auxin under adverse conditions to promote or maintain stem growth. Plant growth and development is closely connected to the regulation of endogenous hormones, especially the polar transport and asymmetric distribution of auxin. The PIN-FORMED (PIN) auxin efflux carrier gene family plays a key role in the polar transport of auxin and then regulates the growth of different plant tissues, which could indicate that the rapid growth of M. micrantha is closely related to this PIN-dependent auxin regulation. In this study, 11 PIN genes were identified and the phylogenetic relationship and structural compositions of the gene family in M. micrantha were analyzed by employing multiple bioinformatic methods. The phylogenetic analysis indicated that the PIN proteins could be divided into five distinct clades. The structural analysis revealed that three putative types of PIN (canonical, noncanonical and semi-canonical) exist among the proteins according to the length and the composition of the hydrophilic domain. The majority of the PINs were involved in the process of axillary bud differentiation and stem response under abiotic stress, indicating that M. micrantha may regulate its growth, development and stress response by regulating PIN expression in the axillary bud and stem, which may help explain its strong growth ability and environmental adaptability. Our study emphasized the structural features and stress response patterns of the PIN gene family and provided useful insights for further study into the molecular mechanism of auxin-regulated growth and control in M. micrantha.

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MtPIN1 and MtPIN3 Play Dual Roles in Regulation of Shade Avoidance Response under Different Environments in Medicago truncatula

Cited by 4 publications

References 61 publications

Clathrin light chains regulate hypocotyl elongation by affecting the polarization of the auxin transporter PIN3 in Arabidopsis

Clathrin light chains regulate hypocotyl elongation by affecting the polarization of the auxin transporter PIN3 in Arabidopsis

Molecular mechanism of leaf adaxial upward curling caused by BpPIN3 suppression in Betula pendula

Genome-Wide Characterization of PIN Auxin Efflux Carrier Gene Family in Mikania micrantha

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