“…The blots were incubated with following primary antibodies with respective dilutions: MUC16 (mouse, 1:1000), MUC16 (mouse, 1:1000), pJAK2 #8082, JAK2 #3230, pSTAT3 #9145, STAT3 #12640, GR #12041, pSrc #2101 (Rabbit, 1:2000, Cell signaling technology), E-cadherin (mouse, 1:1500) and N-cadherin (mouse, 1:1500) antibodies were a kind gift from Dr Keith R Johnson, UNMC, Omaha, NE, USA, CK-18 (mouse, 1:1500, Abcam #668), TSPYL5 (rabbit 1:500, Santa Cruz Technology, #sc-98185), p53 (mouse 1:500, Santa Cruz Technology, #sc-126) and anti-β-actin (mouse 1:5000, Sigma #A1978)) (diluted in 2% BSA in PBS). Similarly, immunoprecipitation assay was performed as described previously (22). The signals were detected with the ECL chemiluminescence kit (Amersham Bioscience, UK).…”