Muc5ac mucin expression during rat skin development

Ferretti, Valeria Alejandra; Segal‐Eiras, Amada; Barbeito, Claudio Gustavo; Croce, María Virginia

doi:10.4081/ejh.2015.2462

Cited by 8 publications

(7 citation statements)

References 33 publications

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“… 3 For example, MUC5AC is expressed in rat embryonic epidermis and may play a protective role in embryonic skin prior to birth before the mucus is replaced with hair. 11 MUC4 also exists in the skin of embryos and disappears late in gestation, which is consistent with a protective role for mucin in human embryos. 12 Based on these reports, we speculate that mucins appear in embryonic skin to play protective and defensive roles but that mucin expression in adult skin is restricted to reduce the risk of cancer progression.…”

supporting

confidence: 69%

The mucin protein MUCL1 regulates melanogenesis and melanoma genes in a manner dependent on threonine content*

Kim

Choi

2021

Br J Dermatol

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Background The regulation of melanogenesis has been investigated as a long-held aim for pharmaceutical manipulations with denotations for malignancy of melanoma. Mucins have a protective function in epithelial organs; however, in the most outer organ, the skin, the role of mucins has not been studied enough. Objectives Our initial hypothesis developed from the identification of correlations between pigmentation and expressions of skin mucins, particularly those existing in skin tissue. We aimed to investigate the action of mucins in human melanocytic cells. Materials and methodsThe expression of mucin proteins in human skin was investigated using microarray data from the Human Protein Atlas consortium (HPA) and the Genotype-Tissue Expression consortium (GTEx) database. Mucin expression was measured at RNA and protein levels in melanoma cells. The findings were further validated and confirmed by analysis of independent experiments. Results We found that the several mucin proteins showed expression in human skin cells and among these, mucin-like protein 1 (MUCL1) showed the highest expression and also clear negative correlation with melanogenesis in epidermal melanocytes. We confirmed the correlations between melanogenesis and MUCL1 by revealing negative correlations in melanocytes with different melanin production, resulting from increased composition of threonine, mucin-conforming amino acid, and increased autophagy-related forkhead-box O signalling. Furthermore, threonine itself affects melanogenesis and metastatic activity in melanoma cells. Conclusions We identified a significant association between MUCL1 and threonine with melanogenesis and metastasis-related genes in melanoma cells. Our results define a novel mechanism of mucin regulation, suggesting diagnostic and preventive roles of MUCL1 in cutaneous melanoma.What is already known about this topic?• Despite considerable advances in radioactive therapeutics or chemotherapeutic approaches for the treatment of abnormal melanogenesis, there are still many caveats to delivery, effectiveness and safety, thus leaving a necessity for more immediate pharmaceutical targets.• Mucins have protective and chemical barrier functions in epithelial organs; however, in the skin, mucin has scarce expression and is known only as a diagnostic aid in skin disorders such as mucinosis.What does this study add?• We provide detailed analysis demonstrating the potential of mucin-like protein 1 (MUCL1), which showed negative correlations in melanocytes with different

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supporting

confidence: 69%

The mucin protein MUCL1 regulates melanogenesis and melanoma genes in a manner dependent on threonine content*

Kim

Choi

2021

Br J Dermatol

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“… 20 The data from the present study successfully fills in the gap of neurotrophin expression in the testis of a seasonal breeder and suggested that NGF system might be actively involved in the seasonal changes in spermatogensis and testicular activity. As we recently found that spermatogensis and testicular function of the wild ground squirrel re-activated during, instead of after, winter hibernation, 30 it will be interesting to further investigate if NGF system also plays a role in this particular process, when the activity of nervous system reaches the low level, for the future research.…”

Section: Discussionmentioning

confidence: 99%

Testicular expression of NGF, TrkA and p75 during seasonal spermatogenesis of the wild ground squirrel (Citellus dauricus Brandt)

et al. 2015

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The nerve growth factor (NGF) not only has an essential effect on the nervous system, but also plays an important role in a variety of non-neuronal systems, such as the reproductive system. The aim of this study was to compare the quality and quantity in expression of NGF and its receptors (TrkA and p75) in testes of the wild ground squirrel during the breeding and nonbreeding seasons. Immunolocalization for NGF was detected mainly in Leydig cells and Sertoli cells in testes of the breeding and nonbreeding seasons. The immunoreactivity of TrkA was highest in the elongated spermatids, whereas p75 in spermatogonia and spermatocytes in testes of the breeding season. In the nonbreeding season testes, TrkA showed positive immunostainings in Leydig cells, spermatogonia and primary spermatocytes, while p75 showed positive signals in spermatogonia and primary spermatocytes. Consistent with the immunohistochemical results, the mean mRNA and protein level of NGF and TrkA were higher in the testes of the breeding season than in non-breeding season, and then decreased to a relatively low level in the nonbreeding season. In addition, the concentration of plasma gonadotropins and testosterone were assayed by radioimmunoassay (RIA), and the results showed a significant difference between the breeding and nonbreeding seasons with higher concentrations in breeding season. In conclusion, these results of this study provide the first evidence on the potential involvement of NGF and its receptor, TrkA and p75 in the seasonal spermatogenesis and testicular function change of the wild ground squirrel.

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“…Interestingly, MUC5AC expression is never detected in adult bowel unless early stages of neoplasia develop (51). Also, expression of MUC5AC protein had been observed early in embryonic epidermis from day 13 of gestation until 7 days after birth, when the surface epidermis became negative, and the expression restricts to secreting sebum cells (65). Expression of secretory mucin MUC5AC is deregulated in different disease conditions as reviewed in Table 2.…”

Section: Dysregulation Of Muc5ac In Benign Pathologiesmentioning

confidence: 99%

Ramifications of secreted mucin MUC5AC in malignant journey: a holistic view

et al. 2018

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Heavily glycosylated secreted mucin MUC5AC, by the virtue of its cysteine-rich repeats, can form inter- and intramolecular disulfide linkages resulting in complex polymers, which in turn craft the framework of the polymeric mucus gel on epithelial cell surfaces. MUC5AC is a molecule with versatile functional implications including barrier functions to epithelial cells, host-pathogen interaction, immune cell attraction to sites of premalignant or malignant lesions and tumor progression in a context-dependent manner. Differential expression, glycosylation and localization of MUC5AC have been associated with a plethora of benign and malignant pathologies. In this era of robust technologies, overexpression strategies and genetically engineered mouse models, MUC5AC is emerging as a potential diagnostic, prognostic and therapeutic target for various malignancies. Considering the clinical relevance of MUC5AC, this review holistically encompasses its genomic organization, domain structure, glycosylation patterns, regulation, functional and molecular connotation from benign to malignant pathologies. Furthermore, we have here explored the incipient and significant experimental tools that are being developed to study this structurally complex and evolutionary conserved gel-forming mucin.

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Muc5ac mucin expression during rat skin development

Cited by 8 publications

References 33 publications

The mucin protein MUCL1 regulates melanogenesis and melanoma genes in a manner dependent on threonine content*

The mucin protein MUCL1 regulates melanogenesis and melanoma genes in a manner dependent on threonine content*

Testicular expression of NGF, TrkA and p75 during seasonal spermatogenesis of the wild ground squirrel (Citellus dauricus Brandt)

Ramifications of secreted mucin MUC5AC in malignant journey: a holistic view

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