MUC5AC overexpression in tear film of neonates

Mantelli, Flavio; Tiberi, Eloisa; Micera, Alessandra; Lambìase, Alessandro; Visintini, F; Bombardieri, Stefano

doi:10.1007/s00417-007-0602-9

Cited by 29 publications

(27 citation statements)

References 20 publications

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“…Even in normal eyes, when the eye is kept open for a longer time, after the complete establishment of TF, TFBU can occur, probably owing to evaporation. [31][32][33][34][35] TFLL [34][35][36][37][38] and secretory mucin 10,38 (MUC5AC) are thought to be components of the TF, contributing to the suppression of evaporation and stability of TF after its establishment, and insufficiency of those components would result in earlier TFBU, even after the complete establishment of TF owing to facilitated evaporation. It should be kept in mind that currently, the capability of TF to resist evaporation and the evaporation-suppressive action of the TFLL are controversial, both in clinical and in vitro studies, and precise clarification of these phenomena is a topic for future research.…”

Section: Tf Bups In Iedementioning

confidence: 99%

Tear Film–Oriented Diagnosis and Tear Film–Oriented Therapy for Dry Eye Based on Tear Film Dynamics

Yokoi

Georgiev

2018

Invest. Ophthalmol. Vis. Sci.

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, 3% diquafosol sodium ophthalmic solution and 2% rebamipide ophthalmic suspension, respectively, appeared first in Japan as prescription drugs for the treatment of dry eye (DE). Since then, not only the diagnosis and treatment but also the understanding of the pathophysiology of DE have greatly advanced, and a new concept of layer-by-layer diagnosis and treatment for DE, respectively termed ''tear-film-oriented diagnosis'' (TFOD) and ''tear-film-oriented therapy'' (TFOT) was born. This new concept is currently in the process of expanding from Japan to other Asian countries. TFOD is the method used for the differential diagnosis of DE, which includes aqueous-deficiency DE (ADDE), decreased wettability DE (DWDE), and increased evaporation DE (IEDE), through the dynamics of tear film (TF) and breakup patterns (BUPs) after the eye is opened. BUPs and/or each diagnosed DE subtype are/is able to distinguish the insufficient components of the ocular surface that are responsible for each BUP in a layer-by-layer fashion. Aqueous fluid, membrane-associated mucins (especially MUC16), and the lipid layer and/or secretory mucins must be insufficient in ADDE, DWDE, and IEDE, respectively, and this allows for a layer-bylayer treatment to be proposed for each BUP via the supplementation of the insufficient components, using the topical therapy currently available. In Japan, TF breakup is regarded as a visible core mechanism for DE, and an abnormal breakup time (i.e., 5 seconds) and symptoms are currently used for the diagnosis of DE. Therefore, TFOD and TFOT could be an ideal and practical pathway for clinicians to manage DE.

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Section: Tf Bups In Iedementioning

confidence: 99%

Tear Film–Oriented Diagnosis and Tear Film–Oriented Therapy for Dry Eye Based on Tear Film Dynamics

Yokoi

Georgiev

2018

Invest. Ophthalmol. Vis. Sci.

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“…Human infants blink spontaneously (SB) at very low rates. Neonates blink about 1-2 blinks/min (Mantelli et al, 2007) and 3-month-old blink about 3-4 blinks/min (Bacher & Smotherman, 2004a). Cross-sectional research (Zametkin, Stevens, & Pittman, 1979) shows a gradual increase in SB rate from infancy to a plateau in young adulthood at about 15-20 blinks/min (Bentivoglio et al, 1997;Doughty & Naase, 2006;Zaman & Doughty, 1997).…”

Section: Introductionmentioning

confidence: 97%

“…The thicker tear film may inhibit eye drying and thus reduce the need for blinks to restore the tear film. Additionally, Mantelli et al (2007) demonstrated that the mucus layer that underlies the tear film in neonates is also thicker than that of adults. More critically, Lawrenson, Birhah, and Murphy (2005) observed infants of ages 3-52 weeks to investigate whether developmental changes in SB were related to tear film morphology and palpebral fissure size.…”

Section: Introductionmentioning

confidence: 99%

Sensitivity of the rate of spontaneous eye blinking to type of stimuli in young infants

Bacher

Allen

2008

Developmental Psychobiology

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Although progress has been made toward understanding the mechanisms of spontaneous eye blinking (SB), few reports focus on the ontogeny of SB. The purpose of the present work was to investigate SB in infants by attempting to manipulate SB and examine potential correlates of SB. Fifty-two infants were observed in a quiet baseline condition then presented with either moving stimuli or a social stimulus. SB, eye movement, body movement and various background variables were measured. Results demonstrate that SB can be manipulated and that SB rate is differentially sensitive to the type of stimulus presented. Eye and body movements did not systematically relate to the rate of SB. Implications for mechanisms of SB regulation are discussed.

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“…Parallel to the superior lipid layer, an increase in mucus expression in infant tears, with the example of MUC5AC, has been described recently. 6 However, infant tear protein content has not been studied as much as the tear film in adults because of the difficulty of collecting from the tear film without provoking reflex tearing and a dilution of the protein content. Proteins regulate important tear functions such as defense 7 and stability in the lipid–aqueous interface.…”

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confidence: 99%

Tear Film Volume and Protein Analysis in Full-term Newborn Infants

Esmaeelpour¹,

Watts²,

Boulton³

et al. 2011

Cornea

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Purpose To investigate infant tear film secretion and protein profile, and to compare major tear proteins, such as immunoglobulin A, lactoferrin, and lysozyme, with those of adult controls. Methods Tears were collected, with a cellulose rod, from 40 healthy infants (19 female infants and 21 male infants, gestational duration: 39.71 ± 1.27 weeks) within 48 hours of birth and 22 adults (10 female infants and 12 male infants, mean age: 24.95 ± 3.63 years). A second collection was obtained from 14 of the infants (8 female infants and 6 male infants, postnatal age: 7.76 ± 6.14 weeks). The tear volume was measured, and protein in the samples was analyzed by Bradford assay and gel electrophoresis. Results Median tear volume (interquartile range) was 0.5μL (0.6– 2 μL) for newborn infants, 2.5μL (1.4–7.75 μL) for these infants at an older age, and 6μL (2.73–12.75 μL) in adults (P < 0.001, Kruskall–Wallis test). Immunoglobulin A concentration was significantly lower in newborns (P < 0.001, analysis of variance). Lipocalin was present in 36% of the newborn tear samples, whereas serum albumin was found in 86%. Mean protein concentration (μg/μL 6 SD) was 10.95 ± 5.51 in the newborns, 12.93 ± 3.99 in the older infants, and 13.04 ± 3.46 in the adults (P > 0.5, analysis of variance). Conclusions This is the first study reporting an investigation of unstimulated infant tears, using a noninvasive collection method. Tear protein content demonstrated that the infant tear film is different to that in adults.

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MUC5AC overexpression in tear film of neonates

Cited by 29 publications

References 20 publications

Tear Film–Oriented Diagnosis and Tear Film–Oriented Therapy for Dry Eye Based on Tear Film Dynamics

Tear Film–Oriented Diagnosis and Tear Film–Oriented Therapy for Dry Eye Based on Tear Film Dynamics

Sensitivity of the rate of spontaneous eye blinking to type of stimuli in young infants

Tear Film Volume and Protein Analysis in Full-term Newborn Infants

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