Mucin−Chitosan Complexes at the Solid−Liquid Interface:  Multilayer Formation and Stability in Surfactant Solutions

Dédinaité, Andra; Lundin, Maria; Macáková, Lubica; Auletta, Tommaso

doi:10.1021/la0511844

Cited by 97 publications

(84 citation statements)

References 45 publications

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“…A noticeable compaction of the protein bi-layer may be expected by the incorporation of LPO molecules into the mucin layer. Similar compaction behaviour of a surface bound BSM layer was previously observed upon subsequent addition of BSA [19] or chitosan [22]. The adsorption conditions might be of high importance in determining the interactions between biopolymers at surfaces since a swelling of an adsorbed BSM layer upon subsequent adsorption of chitosan has been also reported [23,24].…”

Section: Lpo Adsorption On Au-bsm Surfacesupporting

confidence: 55%

Activity of lactoperoxidase when adsorbed on protein layers

Haberska

Svensson

Shleev

et al. 2008

Talanta

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This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues.Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. Contents lists available at ScienceDirect Talanta j o u r n a l h o m e p a g e : w w w . e l s e v i e r . c o m / l o c a t e / t a l a n t a b s t r a c tLactoperoxidase (LPO) is an enzyme, which is used as an antimicrobial agent in a number of applications, e.g., food technology. In the majority of applications LPO is added to a homogeneous product phase or immobilised on product surface. In the latter case, however, the measurements of LPO activity are seldom reported. In this paper we have assessed LPO enzymatic activity on bare and protein modified gold surfaces by means of electrochemistry. It was found that LPO rapidly adsorbs to bare gold surfaces resulting in an amount of LPO adsorbed of 2.9 mg/m 2 . A lower amount of adsorbed LPO is obtained if the gold surface is exposed to bovine serum albumin, bovine or human mucin prior to LPO adsorption. The enzymatic activity of the adsorbed enzyme is in general preserved at the experimental conditions and varies only moderately when comparing bare gold and gold surface pretreated with the selected proteins. The measurement of LPO specific activity, however, indicate that it is about 1.5 times higher if LPO is adsorbed on gold surfaces containing a small amount of preadsorbed mucin in comparison to the LPO directly adsorbed on bare gold.

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Section: Lpo Adsorption On Au-bsm Surfacesupporting

confidence: 55%

Activity of lactoperoxidase when adsorbed on protein layers

Haberska

Svensson

Shleev

et al. 2008

Talanta

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“…It is assumed that the molecule forms multilayers stable at acidic pH [Claesson and Ninham, 1992], resulting in a lower susceptibility of the underlying substrate to erosive demineralisation [Lee et al, 2012]. It can also bind to mucin, occurring in the pellicle structure, with the possibility to form multilayers with the mucin molecule [Dedinaite et al, 2005;Svensson et al, 2006]. Chitosan has also lubricating effects [Guo and Gemeinhart, 2008], which might result in a lower abrasiveness of the toothpaste and, thus, a lower tissue loss after the combined erosive-abrasive challenge.…”

Section: Discussionmentioning

confidence: 99%

Randomised in situ Study on the Efficacy of a Tin/Chitosan Toothpaste on Erosive-Abrasive Enamel Loss

Schlueter

Klimek²,

Ganß³

2013

Caries Res

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Tin is a notable anti-erosive agent, and the biopolymer chitosan has also shown demineralisation-inhibiting properties. Therefore, the anti-erosive/anti-abrasive efficacy of the combination of both compounds was tested under in situ conditions. Twenty-seven volunteers were included in a randomised, double-blind, three-cell crossover in situ trial. Enamel specimens were recessed on the buccal aspects of mandibular appliances, extraorally demineralised (6 × 2 min/day) and intraorally treated with toothpaste slurries (2 × 2 min/day). Within the slurry treatment time, one-half of the specimens received additional intraoral brushing (5 s, 2.5 N). The tested toothpastes included a placebo toothpaste, an experimental NaF toothpaste (1,400 ppm F^-) and an experimental F/Sn/chitosan toothpaste (1,400 ppm F^-, 3,500 ppm Sn²⁺, 0.5% chitosan). The percentage reduction of tissue loss (slurry exposure/slurry exposure + brushing) compared to placebo was 19.0 ± 47.3/21.3 ± 22.4 after use of NaF and 52.5 ± 30.9/50.2 ± 34.3 after use of F/Sn/chitosan. F/Sn/chitosan was significantly more effective than NaF (p ≤ 0.001) and showed good efficacy against erosive and erosive-abrasive tissue loss. This study suggests that the F/Sn/chitosan toothpaste could provide good protection for patients who frequently consume acidic foodstuffs.

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“…Chitosan was also shown to adsorb onto salivary pellicle proteins [Sano et al, 2001;van der Mei et al, 2007]. In the presence of mucin, both chitosan and mucin interact to form firmly attached multilayers [Dedinaite et al, 2005]. Furthermore, in combination with tin, the presence of chitosan promotes a significantly greater reduction of enamel loss both in vitro [Ganss et al, 2012; and in situ [Schlueter et al, 2014].…”

Section: Products Containing Other Proteins or Polymersmentioning

confidence: 99%

The Future of Fluorides and Other Protective Agents in Erosion Prevention

Lussi

Carvalho²

2015

Caries Res

139

136

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The effectiveness of fluoride in caries prevention has been convincingly proven. In recent years, researchers have investigated the preventive effects of different fluoride formulations on erosive tooth wear with positive results, but their action on caries and erosion prevention must be based on different requirements, because there is no sheltered area in the erosive process as there is in the subsurface carious lesions. Thus, any protective mechanism from fluoride concerning erosion is limited to the surface or the near surface layer of enamel. However, reports on other protective agents show superior preventive results. The mechanism of action of tin-containing products is related to tin deposition onto the tooth surface, as well as the incorporation of tin into the near-surface layer of enamel. These tin-rich deposits are less susceptible to dissolution and may result in enhanced protection of the underlying tooth. Titanium tetrafluoride forms a protective layer on the tooth surface. It is believed that this layer is made up of hydrated hydrogen titanium phosphate. Products containing phosphates and/or proteins may adsorb either to the pellicle, rendering it more protective against demineralization, or directly to the dental hard tissue, probably competing with H+ at specific sites on the tooth surface. Other substances may further enhance precipitation of calcium phosphates on the enamel surface, protecting it from additional acid impacts. Hence, the future of fluoride alone in erosion prevention looks grim, but the combination of fluoride with protective agents, such as polyvalent metal ions and some polymers, has much brighter prospects.

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Mucin−Chitosan Complexes at the Solid−Liquid Interface: Multilayer Formation and Stability in Surfactant Solutions

Cited by 97 publications

References 45 publications

Activity of lactoperoxidase when adsorbed on protein layers

Activity of lactoperoxidase when adsorbed on protein layers

Randomised in situ Study on the Efficacy of a Tin/Chitosan Toothpaste on Erosive-Abrasive Enamel Loss

The Future of Fluorides and Other Protective Agents in Erosion Prevention

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