Mucin gene cDNA sequence characterization in chinchilla middle ear epithelium

Kerschner, Joseph E.; Meyer, Tanya K.; Burrows, Amy; Ehrlich, Garth D.; Post, J. Christopher

doi:10.1016/j.ijporl.2006.03.007

Cited by 9 publications

(9 citation statements)

References 25 publications

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“…2A). It was not unprecedented that a rat-specific reagent recognized a chinchilla homologue, as we and others routinely use rat-specific (and human-specific) reagents for immunodetection in the chinchilla model (18,29,40,53,62). Greater than 75% of cells within each culture were CD11c ϩ , as determined by flow cytometry, also comparable to reports of cells cultured from other species.…”

Section: Resultsmentioning

confidence: 99%

Differential Uptake and Processing of a Haemophilus influenzae P5-Derived Immunogen by Chinchilla Dendritic Cells

2008

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Dendritic cells (DCs) are potent antigen-presenting cells involved in the initiation and modulation of immune responses after immunization via their ability to process and present antigen to naive T cells. We wanted to examine the role of DCs in the development of protective immunity against nontypeable Haemophilus influenzae (NTHI)-induced experimental otitis media (OM) after intranasal immunization of chinchillas with an NTHI P5-derived synthetic peptide immunogen called LB1. As chinchilla DCs have not been described, we adapted well-established protocols to induce the differentiation of chinchilla bone marrow precursor cells into DCs, which resulted in cells that were morphologically and phenotypically similar to DCs of other species. In vitro, chinchilla DCs readily internalized LB1, upregulated expression of the maturation markers CD80 and major histocompatibility complex class II, and presented processed LB1 to primed CD3 ؉ T cells, which resulted in antigen-specific T-cell proliferation. In vivo, LB1-activated DCs trafficked from the chinchilla nasal cavity primarily to the nasal-associated lymphoid tissues and were detected in close proximity to CD3 ؉ T cells within this lymphoid aggregate. These data are the first to characterize chinchilla DCs and their functional properties. Furthermore, they suggest an important role for chinchilla DCs in the development of protective immunity against experimental NTHI-induced OM after intranasal immunization.

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Section: Resultsmentioning

confidence: 99%

Differential Uptake and Processing of a Haemophilus influenzae P5-Derived Immunogen by Chinchilla Dendritic Cells

2008

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“…These mucins have been demonstrated in a number of studies, including from our laboratory, to be the primary gel-forming mucins of the MEE [5–8]. In addition, these gel-forming mucins are conserved in the MEE across multiple species including human, rat, mouse and chinchilla [9,10]. Each of these mucins have been shown to be up-regulated in response to non-specific cytokine inflammatory responses [11,12].…”

Section: Introductionmentioning

confidence: 99%

Differential response of gel-forming mucins to pathogenic middle ear bacteria

Kerschner

Hong

Khampang

et al. 2014

International Journal of Pediatric Otorhinolaryngology

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Objective To assess the differential response of the secretory gel forming mucins (GFM) to the most common bacterial pathogens causing otitis media, Streptococcus pneumoniae (SP), nontypeable Haemophilus influenza (NTHi), and Moraxella catarrhalis (Mcat), in a culture model of human middle ear epithelium (HMEEC). Methods In vitro cultured HMEEC was exposed to 5µg/ml of bacterial whole cell lysate (WCL). RNA was extracted to generate cDNA. The expression levels of each of the targeted mucin transcripts, MUC2, MUC5AC, MUC5B and MUC19, were detected by quantitative PCR. Results The submerged HMEEC exposed to NTHi-86028NP WCL demonstrated a significant increase of MUC2, MUC5AC and MUC5B as compared to the control non treated cells while MUC19 transcript level remained unchanged. WCL of additional major OM pathogens significantly increase the transcription of these three mucin genes as well. A combination of NTHi and SP further synergistically induced MUC2 and MUC5AC gene expression however, not all NTHi strains synergized with SP in the induction. Addition of Mcat WCL to the synergized combination of NTHi and SP did not participate in the synergistic response of mucins. Conclusion The specific pathogen combinations were important in determining the degree of synergistic effects to GFM expression. The current data are substantive in guiding future work to extend our understanding of OM pathogens and GFMs.

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“…Studies at our institution and others have demonstrated the applicability of the chinchilla model of OM to human disease as it provides an inexpensive, reproducible middle-ear infection in nearly 100% of inoculated animals that has yielded numerous insights into the molecular pathophysiology and microbiology of mammalian middle ear disease [34-41]. …”

Section: Introductionmentioning

confidence: 99%

Virulence phenotypes of low-passage clinical isolates of Nontypeable Haemophilus influenzae assessed using the chinchilla laniger model of otitis media

et al. 2007

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Background: The nontypeable Haemophilus influenzae (NTHi) are associated with a spectrum of respiratory mucosal infections including: acute otitis media (AOM); chronic otitis media with effusion (COME); otorrhea; locally invasive diseases such as mastoiditis; as well as a range of systemic disease states, suggesting a wide range of virulence phenotypes. Genomic studies have demonstrated that each clinical strain contains a unique genic distribution from a population-based supragenome, the distributed genome hypothesis. These diverse clinical and genotypic findings suggest that each NTHi strain possesses a unique set of virulence factors that contributes to the course of the disease.

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Mucin gene cDNA sequence characterization in chinchilla middle ear epithelium

Cited by 9 publications

References 25 publications

Differential Uptake and Processing of a Haemophilus influenzae P5-Derived Immunogen by Chinchilla Dendritic Cells

Differential Uptake and Processing of a Haemophilus influenzae P5-Derived Immunogen by Chinchilla Dendritic Cells

Differential response of gel-forming mucins to pathogenic middle ear bacteria

Virulence phenotypes of low-passage clinical isolates of Nontypeable Haemophilus influenzae assessed using the chinchilla laniger model of otitis media

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