Mucin Histochemistry in Tumours of Colon, Ovaries and Lung

Ullah, Ehsan

doi:10.4172/2157-7099.1000163

Cited by 10 publications

(5 citation statements)

References 24 publications

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“…Also, mucins constitute a major portion of the tumor area/volume in many cases . However, the rapid detection of mucin is challenging because current standard methods, like histochemical analysis of tumor tissues through PAS/Alcain blue staining, , are invasive and time-consuming and alternative approaches, such as enzyme-linked immunosorbent assays, antibody-mediated field-effect-transistor devices, and aptamer-based fluorometric assays, − require expensive antibody-mediated reactions and complicated labeling steps for signal generation. Thereby, the development of a rapid “one-step” assay for analysis of mucins present in body fluids, tumor biopsy, etc., could enable integration to POC platforms for in vitro diagnostics.…”

Section: Introductionmentioning

confidence: 99%

Phenylboronic Acid Templated Gold Nanoclusters for Mucin Detection Using a Smartphone-Based Device and Targeted Cancer Cell Theranostics

Dutta

Sailapu

Chattopadhyay

et al. 2018

ACS Appl. Mater. Interfaces

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A phenylboronic acid templated gold nanocluster probe was developed to detect biomarker mucin by a noninvasive fluorescence-based method using a point-of-care smartphone-based fluorescence detection device. The gold nanocluster probe is able to detect mucin specifically. The same probe was applied for in vitro targeted bioimaging of HeLa and Hep G2 cancer cells, and it demonstrated specific therapeutic effects toward cancer cells as well as multicellular tumor spheroids imparting theranostic properties. The module is found to be more effective toward HeLa cells, and a pathway of cell death was established using flow-cytometry-based assays.

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Section: Introductionmentioning

confidence: 99%

Phenylboronic Acid Templated Gold Nanoclusters for Mucin Detection Using a Smartphone-Based Device and Targeted Cancer Cell Theranostics

Dutta

Sailapu

Chattopadhyay

et al. 2018

ACS Appl. Mater. Interfaces

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“…Mucins are, therefore, an important factor in tumor cell survival and protection against the immune response of the affected host. An increase in mucin production has been observed in lung cancer [72], and secretory mucins act as a protective mechanism against exogenous cytotoxicity in tumor cells and increase the aggressiveness of a tumor [73]. Secretory mucins can cause web formations and heavily glycosylated membrane-bound mucins.…”

Section: Discussionmentioning

confidence: 99%

A Novel NCI-H69AR Drug-Resistant Small-Cell Lung Cancer Mini-Tumor Model for Anti-Cancer Treatment Screening

Niekerk

Wrzesinski

Steyn

et al. 2023

Cells

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Small-cell lung cancer is a fast-growing carcinoma with a poor prognosis and a high level of relapse due to multi-drug resistance (MDR). Genetic mutations that lead to the overexpression of efflux transporter proteins can contribute to MDR. In vitro cancer models play a tremendous role in chemotherapy development and the screening of possible anti-cancer molecules. Low-cost and simple in vitro models are normally used. Traditional two-dimensional (2D) models have numerous shortcomings when considering the physiological resemblance of an in vivo setting. Three-dimensional (3D) models aim to bridge the gap between conventional 2D models and the in vivo setting. Some of the advantages of functional 3D spheroids include better representation of the in vivo physiology and tumor characteristics when compared to traditional 2D cultures. During this study, an NCI-H69AR drug-resistant mini-tumor model (MRP1 hyperexpressive) was developed by making use of a rotating clinostat bioreactor system (ClinoStar®; CelVivo ApS, Odense, Denmark). Spheroid growth and viability were assessed over a 25-day period to determine the ideal experimental period with mature and metabolically stable constructs. The applicability of this model for anti-cancer research was evaluated through treatment with irinotecan, paclitaxel and cisplatin for 96 h, followed by a 96 h recovery period. Parameters measured included planar surface area measurements, estimated glucose consumption, soluble protein content, intracellular adenosine triphosphate levels, extracellular adenylate kinase levels, histology and efflux transporter gene expression. The established functional spheroid model proved viable and stable during the treatment period, with retained relative hyperexpression of the MRP1 efflux transporter gene but increased expression of the P-gp transporter gene compared to the cells cultured in 2D. As expected, treatment with the abovementioned anti-cancer drugs at clinical doses (100 mg/m2 irinotecan, 80 mg/m2 paclitaxel and 75 mg/m2 cisplatin) had minimal impact on the drug-resistant mini-tumors, and the functional spheroid models were able to recover following the removal of treatment.

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“…Studies have shown that, there is a tendency of increased production of sialomucins in various pathological conditions, with decreased O-acetylation as a common early feature of malignant and premalignant epithelial disorders, particularly in colon. This has been demonstrated both histochemically and biochemically in colorectal cancer, ulcerative colitis and in colonic adenoma 1,3,5,6 .…”

Section: Introductionmentioning

confidence: 90%

“…Expression of mucins is a property of epithelial cell types that exist in relatively harsh environments. Mucin's key characteristic is its ability to form gels; therefore they are a key component in most gel-like secretions, serving functions such as lubrication, cell signaling and forming chemical barriers 3 . In addition, mucins also communicate the information of the external environment to the epithelial cells via cellular signalling through membrane-anchored mucins.…”

Section: Introductionmentioning

confidence: 99%

ALCIAN BLUE pH;

Mwakyoma

Tonya

2014

TPMJ

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Background: Alcian blue (AB) is a cationic dye that stains mucins especially acidic mucins into varying shades of blue colour depending on the pH of the dye. GIT comprises of three main portions, fore-, mid- and hind-gut, which develops from different embryological areas. Their epithelial lining presents with varying degree of diversity including their secretions. Studies have shown that there is a tendency of cellular modification (physical and chemical) including secretions during tumour morphogenesis. With recent increase in GIT tumours especially epithelial tumours, Alcian Blue staining of mucins produced by these tumours at different pH more than conventional pH might give valuable information on the property of these tumours. Setting: The study was conducted in the Histopathology and Morbid Anatomy unit, department of Laboratory services, Muhimbili National Hospital, Tanzania. Study design: This was a hospital based retrospective study, in which archival data and blocks were retrieved. Objective: To determine the effect of pH and pattern of Alcian blue staining on primary GIT epithelial tumours tumours. Materials and Methods: Information on patients were obtained from cancer registry and patient files. Paraffin blocks were retrieved from archive, sections were cut using rotary microtome at 3μm (SAKURA). Haematoxylin and Eosin (H&E) and AB staining at pH 1, 1.5, 2 and 2.5 was done for each case and control. H&E slides were reviewed for confirmation of the diagnosis primary epithelial tumours and AB stained slides were evaluated for staining reaction and graded. Results: Out of 87 GIT primary epithelial tumours which were evaluated, AB staining was positive in 21 (24.1%) cases, the majority of these (11 (52.4%)) were from hindgut. Positive AB staining of GIT epithelial tumours increased as the tumour became more differentiated irrespective of location. Majority of tumours with positive AB staining was observed at pH 2 in GIT epithelial tumours as opposed to the conventional pH of 1 and 2.5 respectively. Conclusions and recommendation: The majority of primary GIT epithelial tumours stained positively at pH 2 irrespective of the location. However tumour differentiation influenced AB staining whereby well-differentiated tumours were mostly positively stained. It is recommended that AB at pH 2 should be applied when staining GIT epithelial tumours rather than conventional pH of 1 and 2.5. However the degree of differentiation should be considered since poorly differentiated tumours are likely to give negative results with AB staining.

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Mucin Histochemistry in Tumours of Colon, Ovaries and Lung

Abstract: Introduction: Mucins implicated in cancers of various organs. The apical epithelial surfaces of mammalian respiratory, gastrointestinal, and reproductive tracts are coated by mucus, a mixture of water, ions, glycoproteins, proteins, and lipids.

Cited by 10 publications

References 24 publications

Phenylboronic Acid Templated Gold Nanoclusters for Mucin Detection Using a Smartphone-Based Device and Targeted Cancer Cell Theranostics

Phenylboronic Acid Templated Gold Nanoclusters for Mucin Detection Using a Smartphone-Based Device and Targeted Cancer Cell Theranostics

A Novel NCI-H69AR Drug-Resistant Small-Cell Lung Cancer Mini-Tumor Model for Anti-Cancer Treatment Screening

ALCIAN BLUE pH;

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