Mucin-Type Glycoproteins

Strous, Ger J.; Dekker, Jan

doi:10.3109/10409239209082559

Cited by 836 publications

(564 citation statements)

References 201 publications

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“…[20][21][22] Mucins are large, highly glycosylated proteins recognized by their tandem repeat domains, which are rich in serine and threonine sites for O-glycosylation. 23 In recent years, membrane-associated mucins have received increasing attention, first as components of carcinoma cell surfaces, 24,25 and later for their roles in the protection of epithelia and other cells. 25,26 In addition to their role in providing barriers that can limit direct access of other cells 26 or large molecules 27,28 to the associated cell surface, recent studies have also implicated them in cellular signaling.…”

Section: Discussionmentioning

confidence: 99%

Low D2-40 immunoreactivity correlates with lymphatic invasion and nodal metastasis in early-stage squamous cell carcinoma of the uterine cervix

Dumoff

Chu

et al. 2005

Modern Pathology

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Lymphatic invasion and nodal metastasis are predictors of shorter disease-free and overall survival in carcinoma of the uterine cervix. The monoclonal antibody D2-40, which reacts with the oncofetal membrane antigen M2A, is a new selective marker for lymphatic endothelium, and has been shown to be useful in identifying the presence of lymphatic invasion in various malignant neoplasms, including cervical carcinoma. However, the reactivity of the tumor cells with D2-40 has not yet been evaluated. In this study, we examined the pattern of D2-40 immunoreactivity in a series of 138 invasive squamous cell carcinomas of the uterine cervix. We correlated the presence and extent of D2-40 immunoreactivity in the tumor cells with various clinicopathologic features, the presence of lymphatic invasion, lymph node metastasis and outcome. Diffuse or focal D2-40 immunoreactivity was present in 17 (12%) and 81 (59%) tumors, respectively, while 40 (29%) tumors showed no immunoreactivity. Lymphatic invasion and nodal metastasis were present in 56 and 29% of tumors, respectively. Tumor emboli within lymphatic spaces and metastatic tumor foci in lymph nodes showed no immunoreactivity in 86 and 80% of the cases, respectively. Lymphatic invasion and nodal metastasis were significantly more common in tumors showing low D2-40 immunoreactivity (Po0.0001 and 0.022, respectively). D2-40 immunoreactivity showed no correlation with any other clinicopathologic features examined, including tumor size, grade and FIGO stage. In univariate analysis low D2-40 immunoreactivity was significantly associated with shorter recurrence-free, but not with overall survival. Our studies suggest that D2-40 immunostaining may serve as a marker for increased risk of lymphatic invasion and tumor recurrence in cervical biopsy material. Further study of the biological function of the M2A antigen may shed some light on the interaction of tumor cells with lymphatics.

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Section: Discussionmentioning

confidence: 99%

Low D2-40 immunoreactivity correlates with lymphatic invasion and nodal metastasis in early-stage squamous cell carcinoma of the uterine cervix

Dumoff

Chu

et al. 2005

Modern Pathology

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“…It has been suggested that sulphated and nonsulphated acid mucins protect against enteric illness in piglets (Strous et al ., 1992;Betscher et al ., 2010); therefore, a detriment in intestinal mucous and decrease in goblet cells secreting acid mucins could increase susceptibility to enteric disturbances (Looft, 2013). Nevertheless, the intestine can react to a harmful environment by increasing the rate of epithelium regeneration (Moeser et al ., 2012).…”

Section: Discussionmentioning

confidence: 99%

Escherichia coli lipopolysaccharide affects intestinal mucin secretion in weaned pigs

Zapata

Rodríguez²,

Ramírez³

et al. 2015

Rev Colomb Cienc Pecu

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El lipopolisacárido de Escherichia coli afecta la secreción de mucinas intestinales en cerdos destetados O lipopolissacárido de Escherichia coli afecta a secreção de mucina intestinal em porcos desmamadosSummary Background: to the best of our knowledge, the effects of lipopolysaccharide (LPS) from Escherichia coli on goblet cells and intestinal mucin secretion of weaned pigs has not been reported, and it is unknown whether these effects could trigger enteritis. Objective: to determine the effect of E . coli LPS on intestinal mucin secretion in weaning piglets. Methods: fifty-two piglets weaned at 21 days of age were fed a basal diet supplemented with four LPS levels (0.0, 0.3, 0.5, and 1.0 µg/mg) during 10 days. Piglets were slaughtered on days 1, 5, 7, and 10 post-weaning and samples of small and large intestine were taken for histochemical staining to determine goblet cell population and type of mucins produced (acidic, sulphated, non-sulphated, or neutral). Results: acidic mucin was reduced on day 5 post-weaning independently of the dietary LPS level supplied to piglets. Recovery of acidic mucins was observed during days 7 and 10 post-weaning. Neutral mucins increased on day 5 and decreased on days 7 and 10 post-weaning. High LPS levels decreased goblet cells population and secretion of all types of mucins. This effect was remarkably high for diet two (D2: 0.5 mg LPS/mg food). Conclusions: early weaning (21 d) and LPS addition to the diet affect mucin secretion and intestinal epithelium integrity by modifying goblet cell populations and their balance between acidic and neutral mucin secretion. These findings explain some abnormalities related with post-weaning diarrhea syndrome and help to explain its pathophysiology.

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“…The most important characteristic of these molecules is that they contain serine-and threonine-rich domains which can be extensively Oglycosylated [6][7][8]. Roughly 70-80 % of mucin dry weight is carbohydrate.…”

Section: Introductionmentioning

confidence: 99%

Sialomucin complex at the rat ocular surface: a new model for ocular surface protection

Price‐Schiavi

Meller

Jing

et al. 1998

Biochemical Journal

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The ocular surface, which is among the most accessible and vulnerable tissues in mammals, is protected by a complex tear film composed of lipid, aqueous and mucin layers. In spite of its importance, the molecular nature of the mucin contribution remains uncertain. Since membrane mucins have been implicated in the protection of other epithelia, we have analysed rat corneal and conjunctival tissues for sialomucin complex (SMC), a membrane mucin found at the apical epithelial cell surfaces in the airway and uterus. Using Northern and Western blot analyses, SMC expression was found in both ocular tissues, being particularly abundant in the cornea. In contrast with the other known membrane mucin, MUC1, SMC was localized more heavily towards the apical surface of the epithelial cells. SMC in ocular surface epithelia was produced in both soluble and membrane forms, the latter being found predominantly in the most superficial cells and at apical surfaces. The soluble form was found loosely adsorbed to apical cell surfaces, particularly of the cornea, as indicated by a mild rinsing protocol. Finally, the tear fluid contained substantial amounts of SMC. From these results, we propose a new model for tear mucin components in which SMC is expressed at the apical ocular surface in both membrane-bound and adsorbed soluble forms to provide a direct protective barrier. SMC secreted into the tear fluid may also participate in maintaining the stability of the preocular tear film by acting with other secreted mucins to determine the physical properties and protective behaviour of the tear film.

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Mucin-Type Glycoproteins

Cited by 836 publications

References 201 publications

Low D2-40 immunoreactivity correlates with lymphatic invasion and nodal metastasis in early-stage squamous cell carcinoma of the uterine cervix

Low D2-40 immunoreactivity correlates with lymphatic invasion and nodal metastasis in early-stage squamous cell carcinoma of the uterine cervix

Escherichia coli lipopolysaccharide affects intestinal mucin secretion in weaned pigs

Sialomucin complex at the rat ocular surface: a new model for ocular surface protection

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