Mucin-Type O-Glycosylation in the Drosophila Nervous System

Itoh, Kazuyoshi; Nishihara, Shoko

doi:10.3389/fnana.2021.767126

Cited by 5 publications

(2 citation statements)

References 58 publications

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“…To identify high-level functions (i.e., KEGG pathways; , 86, 87) enriched in the 973 gene differentially expressed between facultative and non-cleaner species, we performed pathway analysis using pathfindR (88). 47 KEGG pathways were enriched in our differentially expressed genes including several broadly associated with synaptic function and plasticity, neuronal growth, and neurite elongation such as ribosomal biogenesis (89), ubiquitin-mediated proteolysis (90), extracellular matrix receptors (91), O-glycan biosynthesis (92), and mTOR signaling (93) (Supplementary Table S3).…”

Section: Fig 2b)mentioning

confidence: 99%

Shared neural transcriptomic patterns underlie the repeated evolution of mutualistic cleaning behavior in Labridae wrasses

Young¹,

Weitekamp²,

Triki³

et al. 2022

Preprint

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Despite the remarkable diversity of life forms on earth, evolutionary biologists have discovered numerous instances where even distantly related species share astonishing similarities in how they behave, look, and function. Given the importance of happenstance in evolution (e.g., random mutations, genetic drift, environmental stochasticity), it is often assumed that the mechanisms underlying such convergent phenotypes are distinct. Nevertheless, recent discoveries that the same pathways can underlie convergently evolved phenotypes have reinvigorated questions about the predictability of evolution and whether broadly conserved genomic mechanisms facilitate phenotypic convergence. Here, we generated transcriptomes of the putative teleost homologs of the mammalian hippocampus and basolateral amygdala, broadly associated with spatial and social cognition, in six sympatric species of Labridae wrasses that vary in mutualistic cleaning behavior (including three non-cleaning, two facultative cleaning, and one obligate cleaning species) and combined differential gene expression, gene co-expression, and phylogenetic comparative analyses to test two hypotheses about convergent evolution and specialization of mutualistic cleaning behavior. We first identify genes and gene modules exhibiting parallel neurotranscriptomic patterns in the repeated evolution of facultative cleaning. We then examined whether expression and co-expression patterns associated with facultative cleaning are also shared in the obligate cleaner species in our dataset and found evidence for transcriptomic concordance, though no evidence for additional specialization. Taken together, our results provide insights into the convergent evolution and the neuromolecular basis of cooperative behavior and, more generally, illustrate the potential of phylogenetic comparative transcriptomics to unravel the mechanistic underpinnings of the repeated evolution of complex organismal phenotypes.

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Section: Fig 2b)mentioning

confidence: 99%

Shared neural transcriptomic patterns underlie the repeated evolution of mutualistic cleaning behavior in Labridae wrasses

Young¹,

Weitekamp²,

Triki³

et al. 2022

Preprint

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“…However, little is known about the functions and glycoprotein expression of O-GalNAc glycans in the mammalian brain. Studies in drosophila have shown that O-glycans are essential in brain development, morphology, and synaptic function 35 . However, the bulk of drosophila brain O-glycans are unsialylated Tn antigen and T antigen, which are nearly undetectable in the mammalian brain at baseline 18 .…”

Section: Introductionmentioning

confidence: 99%

O-GalNAc glycans enrich in white matter tracts and regulate nodes of Ranvier

Noel,

Suttapitugsakul,

Cummings

et al. 2024

Preprint

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Protein O-glycosylation is a critical modification in the brain, as genetic variants in the pathway are associated with both common and severe neuropsychiatric phenotypes. However, little is known about the most abundant type of O-glycan in the mammalian brain, which are O-GalNAc linked. Here, we determined the spatial localization, protein carriers, and cellular function of O-GalNAc glycans in mouse brain. We observed striking spatial enrichment of O-GalNAc glycans in white matter tracts and at nodes of Ranvier. Glycoproteomic analysis revealed that more than half of the identified O-GalNAc glycans were present on chondroitin sulfate proteoglycans termed lecticans, and display both domain enrichment and site-specific heterogeneity. Though genetic ablation in a single cell type failed to replicate the severe phenotypes seen in congenital disorders, inhibition of O-GalNAc synthesis in neurons reduced binding of Siglec-4, a known regulator neurite growth, and shortened the length of nodes of Ranvier. This work highlights a new function of O-GalNAc glycans in the brain and will inform future studies on their role in development and disease.

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