Mucinous Secretion from Canine Heidenhain Pouch after Stimulation with Food, Pentagastrin and Histamine

Abstract

Principal glycoproteins and amino acids were determined in gastric juice obtained from Heidenhain pouches of Labrador pure-bred dogs. Secretions were stimulated by food, pentagastrin or histamine. Non-stimulated fasting secretions served as controls. All samples showed the presence of galactose, glucosamine, galactosamine, fucose, glucose, mannose and sulfate. Galactose and glucosamine were found in equimolar amounts and the galactose to galactosamine ratio was 2:1. Concentrations of most carbohydrate components studied were higher in fasting than in stimulated secretions. Outputs of some of these components were lower in fasting than in stimulated secretions. Modes of stimulation used did not significantly affect the outputs of principal glycoproteins studied. Compared with human gastric secretions studied by others, using similar methods, certain differences were found particularly in the relationship between some carbohydrate components and between amino acids.

Gastric Mucinous Secretion under Various Conditions of Stimulation in Hypothyroid Dogs

“…Galactose and glucosamine were found in equimolar amounts. A similar relationship between these two components was found in porcine (10) and canine secretions (11). In the present study galactose to glucosamine ratio was also close to one, and the state of hypothyroidism did not affect this relation ship.…”

“…The galactose :galactosamine ratios in human gastric secretions were 2:1, 3:1, 4:1, and 5:1 in different gastric aspirations (22). In a previous study in dogs, this ratio approached 2:1 (11). In the present experiment it was also close to 2:1 with one exception (table II).…”

“…Surgery was performed under Halothane anesthesia, induced and maintained by a Fluotec Mark III anesthetic machine (7,11). Heidenhain pouches of the fundic mucosa were constructed and stainless steel cannulas installed.…”

“…The following carbohydrate components were determined: O-galactose, D-mannose, /.-fucose, /7-glucosamine, /7-galactosamine, O-glucose, and total sugars (10,11). We also analyzed gastric mucous for uronic acid (20), total sulfate and amino acids (10,11).…”

“…Total sulfate was determined with the barium method and color, developed with Sulphonize III solution, was read at 640 jam. Total sugars were measured using the Orcinol method (10,11). We determined amino acids using a Beckman 121 amino acid analyzer (Beckman Instruments, USA) (10,11).…”